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[174559_at] serine/threonine-protein kinase

Description CEK008HYR_rc /REP_DB=TREMBL Accession /GB=D34571 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk8h11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 27      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 20      6grp_total > 17      4grp_total > 23      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > Cu      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1042
1 2 3 4 range
A 1752.7
(3)
P 		749.7
(-2.8)
P 		710.3
(-3)
P 		1401.4
(1)
P 		1042
B 1738.4
(3)
P 		815.6
(-2.9)
P 		799.2
(-3)
P 		1509
(1.5)
P 		939
C 1482.8
(3)
P 		882.7
(-2.2)
P 		784.7
(-3)
P 		1249
(1)
P 		698
avr 1658
(3) 		816
(-2.7) 		764.7
(-3) 		1386.5
(1.2) 		893

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1387
1 2 3 4 5 6 range
A 2134.9
(3)
P 		1127.1
(-1.4)
P 		952.5
(-2.2)
P 		758.6
(-3)
P 		943.2
(-2.2)
P 		994.4
(-2)
P 		1376
B 1629
(3)
P 		1224.6
(0.1)
P 		1177.6
(-0.2)
P 		1166.9
(-0.3)
P 		781.7
(-3)
P 		991.1
(-1.5)
P 		847
C 1431.6
(3)
P 		1252.8
(1.4)
P 		1032.7
(-0.5)
P 		877.5
(-1.9)
P 		747.6
(-3)
P 		1021.1
(-0.6)
P 		684
avr 1731.8
(3) 		1201.5
(-0.5) 		1054.3
(-1.5) 		934.3
(-2.3) 		824.2
(-3) 		1002.2
(-1.8) 		908
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 475
1 2 3 4 range
A 638.1
(3)
P 		162.7
(-3)
P 		185.7
(-2.7)
P 		205.1
(-2.5)
P 		475
B 554.4
(3)
P 		201.4
(-2.7)
P 		185.2
(-3)
P 		321.6
(-0.8)
P 		369
C 490.9
(3)
P 		253.8
(-2.1)
P 		273.5
(-1.7)
P 		210.6
(-3)
P 		280
avr 561.1
(3) 		206
(-3) 		214.8
(-2.9) 		245.8
(-2.3) 		355

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 390
1 2 3 4 5 6 range
A 393.6
(2.8)
P 		400.9
(3)
P 		207.4
(-2.6)
P 		192.4
(-3)
P 		284.6
(-0.3)
P 		242.1
(-1.6)
P 		209
B 566.8
(3)
P 		407.6
(-0.4)
P 		286
(-3)
P 		309.1
(-2.5)
P 		317.6
(-2.3)
P 		369.6
(-1.2)
P 		281
C 530.6
(3)
P 		382.3
(0.5)
P 		262.9
(-1.5)
P 		176.5
(-3)
P 		241.9
(-1.9)
P 		341.8
(-0.2)
P 		354
avr 497
(3) 		396.9
(0.8) 		252.1
(-2.4) 		226
(-3) 		281.4
(-1.8) 		317.8
(-1) 		271
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174559_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22383
Sequence Type Exemplar sequence
Representative Public ID CEK008HYR NCBI
Target Description CEK008HYR_rc /REP_DB=TREMBL Accession /GB=D34571 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk8h11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076092(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076092 NCBI Caenorhabditis elegans protein KINase family member (kin-29) (kin-29) mRNA, complete cds. 11/11 A 
F58H12.1 ENSEMBL cdna:known chromosome:CEL140:X:2843924:2852472:-1 gene:F58H12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2843938-2844349(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine-protein kinase
Gene Symbol kin-29
Ensembl F58H12.1
Entrez Gene 180574 Entrez gene
SwissProt Q21017 EMBL-EBI
WormBase CE28479 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
6950 response to stress inferred from sequence similarity QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from direct assay QuickGO AmiGO
5634 nucleus inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81410 Protein kinase protein 29 [Caenorhabditis elegans] ref|NP_508493.1| protein KINase family member (kin-29) [Caenorhabditis elegans] gb|AAK97497.1| serine/threonine kinase KIN-29 [Caenorhabditis elegans] 0.0
blast CAE58825 Hypothetical protein CBG02036 [Caenorhabditis briggsae] 0.0
blast NP_081774 cDNA sequence BC033915 [Mus musculus] gb|AAH63268.2| CDNA sequence BC033915 [Mus musculus] gb|AAH80688.1| CDNA sequence BC033915 [Mus musculus] 1.0E-82
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-65
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 9.0E-85
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-90
Sequence
Target Sequence 
>C. ELEGANS:174559_AT
gtcagttttccatcaggtcaactcccgaaaagttcgcaagaactgcaaatcactaacctt
gttttgcgtgtacaaacctcccgatacgaaaccgatgatccatccgctagtgtctttctg
ttcacttatttccccctacaactctactttgttcagtaccacaattcctcttccatgttt
cctattcaaactcaccttcatgccttgtactttgttcatgttgtacttttcatttcttaa
atttttattcactgtattatttttttattatttctctattgtttctatcttaaaactcta
agtcaagccccccacatttgaattgtcattacttgtacgtctagtttctgtctcgtgata
ttccttgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAGTTTTCCATCAGGTCAACTCC 186 463 19 Antisense
TTGTTTTGCGTGTACAAACCTCCCG 319 703 77 Antisense
GTGTCTTTCTGTTCACTTATTTCCC 148 487 128 Antisense
CCCCTACAACTCTACTTTGTTCAGT 489 261 151 Antisense
TTGTTCAGTACCACAATTCCTCTTC 249 705 167 Antisense
TGTTTCCTATTCAAACTCACCTTCA 352 561 194 Antisense
CCTTCATGCCTTGTACTTTGTTCAT 184 269 213 Antisense
ATTATTTCTCTATTGTTTCTATCTT 86 15 285 Antisense
AGCCCCCCACATTTGAATTGTCATT 262 83 324 Antisense
ACTTGTACGTCTAGTTTCTGTCTCG 334 103 349 Antisense
TTCTGTCTCGTGATATTCCTTGTTC 681 693 364 Antisense
Sequence Source Affymetrix Proprietary Database