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[174551_at]

Description C39200_rc /REP_DB=TREMBL Accession /GB=C39200 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk524h10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 89      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 75      6grp_total > 96      4grp_total > 61      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 493
1 2 3 4 range
A 1128.6
(3)
P 		847.4
(-0.5)
P 		1033.8
(1.8)
P 		643.7
(-3)
P 		485
B 1016.5
(3)
P 		905
(0.1)
P 		784.7
(-3)
P 		856.8
(-1.1)
P 		232
C 809.3
(0.3)
P 		927.4
(2.6)
P 		950.1
(3)
P 		636.1
(-3)
P 		314
avr 984.8
(3) 		893.3
(1) 		922.9
(1.6) 		712.2
(-3) 		273

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 500
1 2 3 4 5 6 range
A 1080.3
(2.2)
P 		867.6
(-0.3)
P 		649.3
(-3)
P 		773.9
(-1.5)
P 		1020.2
(1.5)
P 		1143.3
(3)
P 		494
B 838.8
(-0)
P 		795.3
(-0.7)
P 		643.1
(-3)
P 		1018.9
(2.7)
P 		984.7
(2.2)
P 		1036.2
(3)
P 		393
C 914.9
(0.5)
P 		733
(-3)
P 		1045
(3)
P 		986.3
(1.9)
P 		942.4
(1)
P 		961.9
(1.4)
P 		312
avr 944.7
(0.7) 		798.6
(-2.6) 		779.1
(-3) 		926.4
(0.3) 		982.4
(1.6) 		1047.1
(3) 		268
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 410.9
(3)
P 		184
(-1.3)
P 		270.3
(0.3)
P 		94.2
(-3)
P 		317
B 324.1
(3)
P 		223.5
(-1.2)
P 		181.8
(-3)
P 		182.6
(-3)
P 		142
C 267.9
(1.3)
P 		266.6
(1.3)
P 		331.1
(3)
P 		107.3
(-3)
P 		224
avr 334.3
(3) 		224.7
(-0.2) 		261.1
(0.9) 		128
(-3) 		206

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 199.1
(-0.9)
P 		308.6
(3)
P 		141.4
(-3)
P 		196.3
(-1)
P 		307.9
(3)
P 		278.4
(1.9)
P 		167
B 291.9
(0.3)
P 		264.7
(-0.3)
P 		156.2
(-3)
P 		269.9
(-0.2)
P 		400.1
(3)
P 		386.4
(2.7)
P 		244
C 339.1
(3)
P 		223.7
(-1.9)
P 		266
(-0.1)
P 		198.4
(-3)
P 		304.9
(1.5)
P 		322
(2.3)
P 		141
avr 276.7
(0.6) 		265.7
(0.1) 		187.9
(-3) 		221.5
(-1.7) 		337.6
(3) 		328.9
(2.7) 		150
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174551_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20923
Sequence Type Exemplar sequence
Representative Public ID C39200 NCBI
Target Description C39200_rc /REP_DB=TREMBL Accession /GB=C39200 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk524h10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065466(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065466 NCBI Caenorhabditis elegans Defective in Cullin Neddylation family member (dcn-1) (dcn-1) mRNA, complete cds. 11/11 None
H38K22.2b ENSEMBL cdna:known chromosome:CEL140:III:4313593:4317547:1 gene:H38K22.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175556 2 1 NM_065466 174551_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4317032-4317501(+) 100.0 100.0
Public Domain and Genome References
Ensembl H38K22.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB54260 Hypothetical protein H38K22.2b [Caenorhabditis elegans] ref|NP_497867.2| Defective in Cullin Neddylation family member (dcn-1) [Caenorhabditis elegans] 1.0E-142
blast CAB54261 Hypothetical protein H38K22.2a [Caenorhabditis elegans] ref|NP_497866.2| Defective in Cullin Neddylation family member (dcn-1) [Caenorhabditis elegans] sp|Q9U3C8|DCN1_CAEEL Defective in cullin neddylation protein 1.0E-136
blast G88424 protein H38K22.2 [imported] - Caenorhabditis elegans 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR005176 EMBL-EBI Protein of unknown function DUF298 3.1E-67
Sequence
Target Sequence 
>C. ELEGANS:174551_AT
ctctatttctctctcatcatttaatctcttctgatccgttgttttttctcgtctccggaa
tcccacttatttctttttgcccatcgtctctttctctattctatccctctctcacctaaa
ggttttgtctctgtctcccagtatgctctctgatcctagttgtctctcgctacatttccg
ttacttttcgaccatattactctctccctctgcacatactctttgacgggttgctgcctc
atttctctatctctctccatctctcttcttccaagaagaagatcacaatgaaaagtagaa
aaatgataaagtatggaaaaagttaagaatatttttcatttgagtatttatgtgaatcct
gtaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCTATTTCTCTCTCATCATTTAAT 126 231 41 Antisense
CTCTCTCATCATTTAATCTCTTCTG 392 231 49 Antisense
TATCCCTCTCTCACCTAAAGGTTTT 608 619 142 Antisense
CACCTAAAGGTTTTGTCTCTGTCTC 155 197 153 Antisense
CAGTATGCTCTCTGATCCTAGTTGT 61 207 179 Antisense
TCGCTACATTTCCGTTACTTTTCGA 195 595 207 Antisense
CATTTCCGTTACTTTTCGACCATAT 626 213 213 Antisense
TACTTTTCGACCATATTACTCTCTC 531 645 222 Antisense
CCCTCTGCACATACTCTTTGACGGG 427 259 246 Antisense
CACATACTCTTTGACGGGTTGCTGC 406 195 253 Antisense
TTGAGTATTTATGTGAATCCTGTAA 541 705 380 Antisense
Sequence Source Affymetrix Proprietary Database