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[174547_at]

Description C52141_rc /REP_DB=TREMBL Accession /GB=C52141 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk238g11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 24      4grp_high_8_8 > 55      6grp_highest_nonorm > 80      6grp_total > 02      4grp_total > 56      6grp_highest_sub > 02      6grp_high1_sub > 02      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 692
1 2 3 4 range
A 63.3
(-2.9)
P 		392.7
(-0)
P 		732.1
(3)
P 		56.4
(-3)
A 		676
B 46.4
(-3)
P 		436.8
(0.4)
P 		737.2
(3)
P 		52.6
(-2.9)
A 		691
C 45.1
(-3)
M 		630.5
(2.8)
P 		653.9
(3)
P 		97.8
(-2.5)
P 		609
avr 51.6
(-3) 		486.7
(1) 		707.7
(3) 		68.9
(-2.8) 		656

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 192.1
(-0.8)
P 		346.2
(3)
P 		220.2
(-0.1)
P 		210.4
(-0.3)
P 		264
(1)
P 		101
(-3)
P 		245
B 323.5
(3)
P 		301.7
(2.3)
P 		148.8
(-3)
P 		238.7
(0.1)
P 		246.9
(0.4)
P 		202
(-1.2)
P 		175
C 251.7
(0.4)
P 		323.8
(3)
P 		154.7
(-3)
P 		163
(-2.7)
P 		159.3
(-2.8)
P 		179.2
(-2.1)
P 		169
avr 255.8
(0.5) 		323.9
(3) 		174.6
(-2.5) 		204
(-1.4) 		223.4
(-0.7) 		160.7
(-3) 		163
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 23.1
(-2.5)
P 		85.2
(-0.5)
P 		191.4
(3)
P 		8.3
(-3)
A 		183
B 14.8
(-2.9)
P 		107.9
(0.6)
P 		170.8
(3)
P 		11.2
(-3)
A 		160
C 14.9
(-3)
M 		181.3
(1.7)
P 		227.9
(3)
P 		16.5
(-3)
P 		213
avr 17.6
(-2.8) 		124.8
(0.7) 		196.7
(3) 		12
(-3) 		185

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 35.4
(-2.3)
P 		123.1
(3)
P 		48
(-1.6)
P 		53.4
(-1.2)
P 		79.7
(0.4)
P 		24.6
(-3)
P 		99
B 112.6
(3)
P 		100.4
(2)
P 		36.1
(-3)
P 		63.2
(-0.9)
P 		100.3
(2)
P 		75.3
(0.1)
P 		77
C 93.3
(2.5)
P 		98.8
(3)
P 		39.4
(-2.4)
P 		32.8
(-3)
P 		51.6
(-1.3)
P 		60
(-0.5)
P 		66
avr 80.4
(0.6) 		107.4
(3) 		41.2
(-3) 		49.8
(-2.2) 		77.2
(0.3) 		53.3
(-1.9) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174547_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20517
Sequence Type Exemplar sequence
Representative Public ID C52141 NCBI
Target Description C52141_rc /REP_DB=TREMBL Accession /GB=C52141 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk238g11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064637(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064637 NCBI Caenorhabditis elegans F19H8.4 (F19H8.4) mRNA, complete cds. 11/11 None
F19H8.4 ENSEMBL cdna:known chromosome:CEL140:II:14616020:14622480:1 gene:F19H8.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14622140-14622420(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F19H8.4
Entrez Gene 175122 Entrez gene
SwissProt O45381 EMBL-EBI
WormBase CE32632 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07585 Hypothetical protein F19H8.4 [Caenorhabditis elegans] ref|NP_497038.2| F19H8.4 [Caenorhabditis elegans] 0.0
blast CAE65910 Hypothetical protein CBG11077 [Caenorhabditis briggsae] 0.0
blast CAE17803 Hypothetical protein F19H8.5 [Caenorhabditis elegans] ref|NP_001022099.1| F19H8.5 [Caenorhabditis elegans] 5.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174547_AT
ttaccaacctctctcaaaatttcaacctaaacccagtcaccgtcctggtgacgcccacaa
ttaaaaaattaaatttcccatttcacggaaaacaaaaaaaacatgcgttggaattttgtt
gccttggtttgttttaacctttcaaaaattgacgataatctgtttactggtgatttctca
gtttttctacacttaatcatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTACCAACCTCTCTCAAAATTTCAA 392 663 59 Antisense
TCTCAAAATTTCAACCTAAACCCAG 240 613 70 Antisense
TAAACCCAGTCACCGTCCTGGTGAC 334 637 86 Antisense
TCCTGGTGACGCCCACAATTAAAAA 286 591 101 Antisense
ACATGCGTTGGAATTTTGTTGCCTT 679 103 159 Antisense
GCGTTGGAATTTTGTTGCCTTGGTT 663 289 163 Antisense
TGGAATTTTGTTGCCTTGGTTTGTT 114 545 167 Antisense
TTGACGATAATCTGTTTACTGGTGA 559 703 207 Antisense
AATCTGTTTACTGGTGATTTCTCAG 419 169 215 Antisense
GATTTCTCAGTTTTTCTACACTTAA 452 427 230 Antisense
CAGTTTTTCTACACTTAATCATTTT 701 205 237 Antisense
Sequence Source Affymetrix Proprietary Database