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[174539_at]

Description CEC890_rc /REP_DB=TREMBL Accession /GB=C07890 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk168e10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      6grp_highest_nonorm > 70      6grp_total > 23      4grp_total > 61      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 400
1 2 3 4 range
A 460.8
(3)
P 		423.1
(2.3)
P 		403.6
(2)
P 		121.1
(-3)
A 		340
B 400.1
(3)
P 		345.3
(2)
P 		300.8
(1.2)
P 		64.1
(-3)
M 		336
C 463.6
(3)
P 		440.5
(2.6)
P 		436.5
(2.5)
P 		146.1
(-3)
A 		318
avr 441.5
(3) 		403
(2.3) 		380.3
(1.9) 		110.4
(-3) 		331

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 296
1 2 3 4 5 6 range
A 423
(3)
P 		362.2
(1.7)
P 		326.2
(1)
P 		346.9
(1.4)
P 		248.4
(-0.6)
P 		132.2
(-3)
P 		291
B 428.2
(3)
P 		351.5
(1.1)
P 		387.6
(2)
P 		267.6
(-0.9)
P 		180.7
(-3)
P 		241.1
(-1.5)
P 		248
C 375
(3)
P 		299.3
(0.9)
P 		301.6
(1)
P 		252.2
(-0.4)
P 		156.1
(-3)
P 		239.9
(-0.7)
P 		219
avr 408.7
(3) 		337.7
(1) 		338.5
(1) 		288.9
(-0.4) 		195.1
(-3) 		204.4
(-2.7) 		214
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 167.8
(3)
P 		91.8
(-0)
P 		105.5
(0.5)
P 		17.7
(-3)
A 		150
B 127.6
(3)
P 		85.3
(0.8)
P 		69.7
(-0.1)
P 		13.7
(-3)
M 		114
C 153.5
(3)
P 		126.7
(1.8)
P 		152.2
(2.9)
P 		24.6
(-3)
A 		129
avr 149.6
(3) 		101.3
(0.8) 		109.1
(1.1) 		18.7
(-3) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 78
(-0.2)
P 		128.8
(3)
P 		71
(-0.6)
P 		88
(0.5)
P 		74.9
(-0.3)
P 		32.2
(-3)
P 		97
B 149
(3)
P 		117
(0.5)
P 		94.1
(-1.2)
P 		70.9
(-3)
P 		73.4
(-2.8)
P 		89.9
(-1.5)
P 		78
C 139
(3)
P 		91.3
(-0.2)
P 		76.8
(-1.2)
P 		50.7
(-3)
P 		50.5
(-3)
P 		80.3
(-1)
P 		89
avr 122
(3) 		112.4
(2) 		80.6
(-1.5) 		69.9
(-2.6) 		66.3
(-3) 		67.5
(-2.9) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174539_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22052
Sequence Type Exemplar sequence
Representative Public ID CEC890 NCBI
Target Description CEC890_rc /REP_DB=TREMBL Accession /GB=C07890 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk168e10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076940(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076940 NCBI Caenorhabditis elegans C14F5.4 (C14F5.4) mRNA, complete cds. 11/11 A 
C14F5.4 ENSEMBL cdna:known chromosome:CEL140:X:7956161:7958245:-1 gene:C14F5.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000042706 8/11 Cross Hyb Matching Probes None
GENEFINDER00000042711 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7956215-7956539(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C14F5.4
Entrez Gene 181054 Entrez gene
SwissProt Q09972 EMBL-EBI
WormBase CE30370 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.16037.1.S1_AT similar to Sideroflexin-2 cfa
DROSOPHILA_2:1631456_AT dm
DROSGENOME1:149029_AT dm
CHICKEN:GGA.12531.1.S1_AT sideroflexin 2 gga
HU35KSUBC:RC_AA496127_AT sideroflexin 2 hs
U133_X3P:HS.44070.0.A1_3P_AT sideroflexin 2 hs
HG-U133_PLUS_2:227560_AT sideroflexin 2 hs
HG-U133B:227560_AT sideroflexin 2 hs
HG-U95B:45596_AT sideroflexin 2 hs
MG-U74AV2:103706_AT sideroflexin 2 mm
MOE430A:1435896_AT sideroflexin 2 mm
MOUSE430A_2:1435896_AT sideroflexin 2 mm
MOUSE430_2:1435896_AT sideroflexin 2 mm
MU11KSUBA:AA189555_S_AT sideroflexin 2 mm
MG-U74AV2:160967_AT sideroflexin 2 mm
MG-U74CV2:166322_R_AT sideroflexin 2 mm
MOE430A:1449853_AT sideroflexin 2 mm
MOUSE430A_2:1449853_AT sideroflexin 2 mm
MOUSE430_2:1449853_AT sideroflexin 2 mm
MOE430B:1439966_X_AT Sideroflexin 2, mRNA (cDNA clone MGC:30418 IMAGE:5043343) mm
MOUSE430_2:1439966_X_AT Sideroflexin 2, mRNA (cDNA clone MGC:30418 IMAGE:5043343) mm
MOE430B:1440786_X_AT Sideroflexin 2, mRNA (cDNA clone MGC:30418 IMAGE:5043343) mm
MOUSE430_2:1440786_X_AT Sideroflexin 2, mRNA (cDNA clone MGC:30418 IMAGE:5043343) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8324 cation transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68404 Hypothetical protein C14F5.4 [Caenorhabditis elegans] ref|NP_509341.2| C14F5.4 [Caenorhabditis elegans] 0.0
blast CAE70326 Hypothetical protein CBG16855 [Caenorhabditis briggsae] 1.0E-175
blast AAT27471 mitochondrial sideroflexin 2 [Steinernema carpocapsae] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR004686 EMBL-EBI Tricarboxylate/iron carrier 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509341.2 4 90-112,141-163,224-246,261-283
C14F5.4 4 90-112,141-163,224-246,261-283
Sequence
Target Sequence 
>C. ELEGANS:174539_AT
ccaactcaattggctttaacttttgtgatttatggtgcaatgattcctgtcggatgcgct
ctcttccctcagcaaaactctgtgaagctttcaacattgaaaagatgggagcccgaagcc
tttgaaaaactgaaaaatatcaagggagatcgagtgtacttcaacaaaggattataagcg
atttatgagcttttagttaactaaacatagatttcagcttaatttatttccgtgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAACTCAATTGGCTTTAACTTTTG 605 275 16 Antisense
GATTTATGGTGCAATGATTCCTGTC 393 429 42 Antisense
CAATGATTCCTGTCGGATGCGCTCT 372 183 53 Antisense
CTCTTCCCTCAGCAAAACTCTGTGA 650 229 76 Antisense
AACTCTGTGAAGCTTTCAACATTGA 188 141 91 Antisense
AAAGATGGGAGCCCGAAGCCTTTGA 682 113 116 Antisense
GGAGCCCGAAGCCTTTGAAAAACTG 585 521 123 Antisense
GGGAGATCGAGTGTACTTCAACAAA 210 495 159 Antisense
AGCGATTTATGAGCTTTTAGTTAAC 551 75 192 Antisense
TAACTAAACATAGATTTCAGCTTAA 74 639 213 Antisense
TTCAGCTTAATTTATTTCCGTGCAA 152 687 228 Antisense
Sequence Source Affymetrix Proprietary Database