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[174527_at]

Description CEK002G4R_rc /REP_DB=TREMBL Accession /GB=D27361 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk2g4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 77      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 95      6grp_total > 78      4grp_total > 61      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 453.6
(1.8)
P 		433.1
(1.1)
P 		490.2
(3)
P 		308.3
(-3)
P 		182
B 553.9
(3)
P 		510.3
(2)
P 		391.4
(-0.6)
P 		280.5
(-3)
P 		273
C 500
(3)
P 		460.9
(2.1)
P 		478.8
(2.5)
P 		237
(-3)
P 		263
avr 502.5
(3) 		468.1
(2.1) 		453.5
(1.7) 		275.3
(-3) 		227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1597
1 2 3 4 5 6 range
A 513.2
(-1.9)
P 		405.7
(-2.4)
P 		289.7
(-3)
P 		403.9
(-2.4)
P 		915
(0.2)
P 		1467.7
(3)
P 		1178
B 484.4
(-2)
P 		455.8
(-2.1)
P 		209.4
(-3)
P 		533.2
(-1.8)
P 		636.8
(-1.4)
P 		1805.9
(3)
P 		1597
C 435.9
(-2.6)
P 		425.8
(-2.6)
P 		339.4
(-3)
P 		444.8
(-2.5)
P 		648.7
(-1.6)
P 		1681.7
(3)
P 		1342
avr 477.8
(-2.1) 		429.1
(-2.3) 		279.5
(-3) 		460.6
(-2.2) 		733.5
(-1) 		1651.8
(3) 		1372
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 165.2
(3)
P 		94
(-0.6)
P 		128.2
(1.2)
P 		45.1
(-3)
P 		120
B 176.7
(3)
P 		126
(0.4)
P 		90.7
(-1.4)
P 		59.8
(-3)
P 		117
C 165.5
(2.9)
P 		132.5
(1.4)
P 		166.9
(3)
P 		40
(-3)
P 		127
avr 169.1
(3) 		117.5
(0.4) 		128.6
(1) 		48.3
(-3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 623
1 2 3 4 5 6 range
A 94.6
(-2.4)
P 		144.3
(-1.3)
P 		63.1
(-3)
P 		102.4
(-2.2)
P 		276.1
(1.3)
P 		357.3
(3)
P 		294
B 168.5
(-1.9)
P 		151.7
(-2)
P 		50.9
(-3)
P 		141.2
(-2.1)
P 		258.7
(-1)
P 		673.4
(3)
P 		623
C 161.6
(-2.1)
P 		129.9
(-2.5)
P 		86.4
(-3)
P 		89.4
(-3)
P 		209.9
(-1.4)
P 		563
(3)
P 		477
avr 141.6
(-2) 		142
(-2) 		66.8
(-3) 		111
(-2.4) 		248.2
(-0.7) 		531.2
(3) 		464
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174527_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21545
Sequence Type Exemplar sequence
Representative Public ID CEK002G4R NCBI
Target Description CEK002G4R_rc /REP_DB=TREMBL Accession /GB=D27361 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk2g4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028140(10), NM_001028141(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028140 NCBI Caenorhabditis elegans Zygotic epidermal ENclosure defective family member (zen-4) (zen-4) mRNA, complete cds. 10/11 None
NM_001028141 NCBI Caenorhabditis elegans Zygotic epidermal ENclosure defective family member (zen-4) (zen-4) mRNA, complete cds. 11/11 None
M03D4.1c ENSEMBL cdna:known chromosome:CEL140:IV:6118208:6121745:1 gene:M03D4.1 10/11 A 
M03D4.1d ENSEMBL cdna:known chromosome:CEL140:IV:6120956:6121704:1 gene:M03D4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177374 4 2 NM_001028138 192303_s_at (11)
NM_001028139 192303_s_at (11)
NM_001028140 174527_at (10), 192303_s_at (11)
NM_001028141 174527_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6121028-6121741(+) 95.5 100.0
Public Domain and Genome References
Ensembl M03D4.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM97993 Zygotic epidermal enclosure defective protein 4, isoform c [Caenorhabditis elegans] ref|NP_001023311.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] 0.0
blast AAC24406 Zygotic epidermal enclosure defective protein 4, isoform a [Caenorhabditis elegans] ref|NP_001023309.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] gb|AAC33437.1| kinesin-like protein ZEN-4a [Caenorhabditis elegans] 0.0
blast AAC24407 Zygotic epidermal enclosure defective protein 4, isoform b [Caenorhabditis elegans] ref|NP_001023310.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] gb|AAC33438.1| kinesin-like protein ZEN-4b [Caenorhabditis elegans] 0.0
blast AAM97994 Zygotic epidermal enclosure defective protein 4, isoform d [Caenorhabditis elegans] ref|NP_001023312.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] 5.0E-17
blast AAM97993 Zygotic epidermal enclosure defective protein 4, isoform c [Caenorhabditis elegans] ref|NP_001023311.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] 1.0E-9
blast AAC24406 Zygotic epidermal enclosure defective protein 4, isoform a [Caenorhabditis elegans] ref|NP_001023309.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] gb|AAC33437.1| kinesin-like protein ZEN-4a [Caenorhabditis elegans] 1.0E-9
Protein Domains
Database ID Description E-Value
scop a.2.11.Fe,Mn superoxide dismutase (SOD), N-terminal domain All alpha proteins; Long alpha-hairpin; Fe,Mn superoxide dismutase (SOD), N-terminal domain; Fe,Mn superoxide dismutase (SOD), N-terminal domain 1.39999997615814
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023312.1 1 5-27
M03D4.1d 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:174527_AT
gaagctgctctttttacatttttatgtgtttgttggaaatgtaattccaacagtttccgg
tggcactgccgtgttcttcaacgatatcgagagactcactcacgaatctccgagcacacg
aaagtaattataagtgccgtctttgatctttttacctccatcctgctgcattttcatgtc
aaatttgtaccatactcttcaaattttctccaaatatttattaatttattatgtttctca
ttcataatcccattttccgaactcgaagagaactcttgtttttttgtagacctcaccgca
tttttatgatctttcacttcttcgtctttttctccttctacgcacaattttttgatatcg
atctgaagaactctgttcatttttattcccgacgatgatttgtgtacta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCTGCTCTTTTTACATTTTTAT 512 343 38 Antisense
TAAGTGCCGTCTTTGATCTTTTTAC 128 637 168 Antisense
TGTACCATACTCTTCAAATTTTCTC 693 559 223 Antisense
TTCTCATTCATAATCCCATTTTCCG 518 691 272 Antisense
TAATCCCATTTTCCGAACTCGAAGA 499 635 282 Antisense
TTGTTTTTTTGTAGACCTCACCGCA 320 705 313 Antisense
AGACCTCACCGCATTTTTATGATCT 189 69 325 Antisense
TTTTATGATCTTTCACTTCTTCGTC 48 677 339 Antisense
TTCTCCTTCTACGCACAATTTTTTG 599 691 367 Antisense
ACTCTGTTCATTTTTATTCCCGACG 275 99 407 Antisense
ATTCCCGACGATGATTTGTGTACTA 387 9 422 Antisense
Sequence Source Affymetrix Proprietary Database