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[174520_s_at] guanylate cyclase

Description C59452_rc /REP_DB=TREMBL Accession /GB=C59452 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk431d11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 44      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 90      6grp_total > 10      4grp_total > 11      6grp_highest_sub > 02      6grp_cgc > 54      6grp_high1_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 218.4
(-0.4)
P 		124.1
(-2.2)
P 		82.1
(-3)
P 		400.5
(3)
P 		318
B 87.7
(-2.9)
P 		193.7
(-0.9)
P 		84.4
(-3)
P 		403.6
(3)
P 		319
C 170.6
(-1.4)
P 		85.6
(-3)
P 		102.1
(-2.7)
P 		398.1
(3)
P 		313
avr 158.9
(-1.7) 		134.5
(-2.1) 		89.5
(-3) 		400.7
(3) 		311

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 166
1 2 3 4 5 6 range
A 90.8
(-3)
P 		256.9
(3)
P 		123
(-1.8)
P 		114.6
(-2.1)
P 		114.2
(-2.2)
P 		165.4
(-0.3)
P 		166
B 141.5
(3)
P 		103.7
(-1.7)
P 		97.3
(-2.4)
P 		121
(0.5)
P 		92.8
(-3)
P 		96.1
(-2.6)
P 		49
C 105
(-2.6)
P 		239
(3)
P 		210.5
(1.8)
P 		134.5
(-1.3)
P 		94.4
(-3)
P 		134.4
(-1.3)
P 		145
avr 112.4
(-2.3) 		199.9
(3) 		143.6
(-0.4) 		123.4
(-1.6) 		100.5
(-3) 		132
(-1.1) 		99
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 79.5
(3)
P 		26.9
(-2.4)
P 		21.5
(-3)
P 		58.6
(0.8)
P 		58
B 28
(-2.2)
P 		47.8
(-0.5)
P 		19.6
(-3)
P 		86
(3)
P 		66
C 56.5
(1.5)
P 		24.6
(-3)
P 		35.6
(-1.4)
P 		67.1
(3)
P 		43
avr 54.7
(0.9) 		33.1
(-2) 		25.6
(-3) 		70.6
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 16.7
(-3)
P 		91.4
(3)
P 		26.8
(-2.2)
P 		29.1
(-2)
P 		34.5
(-1.6)
P 		40.3
(-1.1)
P 		75
B 49.2
(3)
P 		34.5
(-0.4)
P 		23.6
(-3)
P 		32
(-1)
P 		37.7
(0.3)
P 		35.9
(-0.1)
P 		26
C 38.9
(-1.4)
P 		72.9
(3)
P 		53.6
(0.5)
P 		27
(-3)
P 		30.6
(-2.5)
P 		45
(-0.6)
P 		46
avr 34.9
(-2.1) 		66.3
(3) 		34.7
(-2.1) 		29.4
(-3) 		34.3
(-2.2) 		40.4
(-1.2) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174520_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21857
Sequence Type Exemplar sequence
Representative Public ID C59452 NCBI
Target Description C59452_rc /REP_DB=TREMBL Accession /GB=C59452 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk431d11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073609(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073609 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-33) (gcy-33) mRNA, complete cds. 11/11 A 
F57F5.2 ENSEMBL cdna:known chromosome:CEL140:V:11998456:12004364:-1 gene:F57F5.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11998466-11999298(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanylate cyclase
Gene Symbol gcy-33
Ensembl F57F5.2
Entrez Gene 179644 Entrez gene
WormBase CE34891 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01418 Hypothetical protein F57F5.2 [Caenorhabditis elegans] emb|CAB00103.2| Hypothetical protein F57F5.2 [Caenorhabditis elegans] ref|NP_506010.2| Guanylyl CYclase family member (gcy-33) [Caenorhabditis elegans] gb|AAP32289.1| soluble guanylyl cyclase GCY-33 [Caenorhabditis elegans] sp|P90895|GCY33_CAEEL Soluble guanylate cyclase gcy-33 0.0
blast CAE75366 Hypothetical protein CBG23350 [Caenorhabditis briggsae] 0.0
blast BAD89804.1 soluble guanylyl cyclase beta-3 [Apis mellifera] ref|NP_001012645.1| soluble guanylyl cyclase beta-3 [Apis mellifera] 1.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.1E-41
Sequence
Target Sequence 
>C. ELEGANS:174520_S_AT
aaataggagatgcaatttccaaaaaaaaattggaaaaggaagattcaaactcatcgatga
gcagtttggacgaaagaacaacttctgcaaaacctacaacgacacgtcgacttctcaatc
aaaaagatttggaaaaagagaaaaagcgatccagtatggcaggatcttctgtgacatctt
cttctgcacattctcattcaatcaggagcaagaaagatactcgtgataaatcaagatgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATAGGAGATGCAATTTCCAAAAA 168 115 13 Antisense
ACTCATCGATGAGCAGTTTGGACGA 79 101 61 Antisense
CTGCAAAACCTACAACGACACGTCG 709 233 97 Antisense
ACCTACAACGACACGTCGACTTCTC 23 3 104 Antisense
GACACGTCGACTTCTCAATCAAAAA 656 367 113 Antisense
AGCGATCCAGTATGGCAGGATCTTC 201 83 157 Antisense
CAGGATCTTCTGTGACATCTTCTTC 68 209 172 Antisense
TCTTCTGCACATTCTCATTCAATCA 481 617 192 Antisense
GCACATTCTCATTCAATCAGGAGCA 248 317 198 Antisense
TCAGGAGCAAGAAAGATACTCGTGA 87 601 214 Antisense
GATACTCGTGATAAATCAAGATGCA 65 423 228 Antisense
Sequence Source Affymetrix Proprietary Database