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[174499_at]

Description CEK006F3R_rc /REP_DB=TREMBL Accession /GB=D27962 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk6f3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 15      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 50      6grp_total > 05      4grp_total > 23      6grp_highest_sub > De      6grp_cgc > 42      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 836
1 2 3 4 range
A 1117.4
(3)
P 		281.6
(-3)
P 		524.1
(-1.3)
P 		667.7
(-0.2)
P 		836
B 765.9
(3)
P 		421
(-1.9)
P 		342.7
(-3)
P 		534.2
(-0.3)
P 		423
C 617.8
(-0.4)
P 		496.9
(-1.7)
P 		364.2
(-3)
P 		960.6
(3)
P 		596
avr 833.7
(3) 		399.8
(-3) 		410.3
(-2.9) 		720.8
(1.4) 		434

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 631
1 2 3 4 5 6 range
A 918.9
(3)
P 		639.4
(0.3)
P 		370.5
(-2.2)
P 		287.8
(-3)
P 		668.3
(0.6)
P 		334.5
(-2.6)
P 		631
B 591.7
(1)
P 		697
(3)
P 		535.5
(-0.1)
P 		424.7
(-2.2)
P 		384.7
(-3)
P 		404.4
(-2.6)
P 		312
C 599.2
(0.7)
P 		775.6
(3)
P 		437.4
(-1.5)
P 		327.2
(-2.9)
P 		439.3
(-1.4)
P 		320.9
(-3)
P 		455
avr 703.3
(3) 		704
(3) 		447.8
(-1.3) 		346.6
(-3) 		497.4
(-0.5) 		353.3
(-2.9) 		357
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 346
1 2 3 4 range
A 406.8
(3)
P 		61.1
(-3)
P 		137
(-1.7)
P 		97.7
(-2.4)
P 		346
B 244.2
(3)
P 		104
(-2.1)
P 		79.4
(-3)
P 		113.8
(-1.7)
P 		165
C 204.5
(3)
P 		142.9
(-1.8)
P 		126.9
(-3)
P 		162
(-0.3)
P 		78
avr 285.2
(3) 		102.7
(-3) 		114.4
(-2.6) 		124.5
(-2.3) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 169.4
(0.7)
P 		227.4
(3)
P 		80.7
(-2.7)
P 		73
(-3)
P 		201.7
(2)
P 		81.4
(-2.7)
P 		154
B 205.9
(1.7)
P 		232
(3)
P 		130
(-2.1)
P 		112.5
(-3)
P 		156.3
(-0.8)
P 		150.8
(-1.1)
P 		120
C 222.1
(2.5)
P 		236.7
(3)
P 		111.3
(-1.4)
P 		65.8
(-3)
P 		142.1
(-0.3)
P 		107.4
(-1.5)
P 		171
avr 199.1
(1.7) 		232
(3) 		107.3
(-2) 		83.8
(-3) 		166.7
(0.4) 		113.2
(-1.8) 		148
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174499_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21264
Sequence Type Exemplar sequence
Representative Public ID CEK006F3R NCBI
Target Description CEK006F3R_rc /REP_DB=TREMBL Accession /GB=D27962 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk6f3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069075(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069075 NCBI Caenorhabditis elegans F42A9.1a (kinase) mRNA, complete cds. 11/11 None
F42A9.1a ENSEMBL cdna:known chromosome:CEL140:IV:8631415:8643986:1 gene:F42A9.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177667 2 1 NM_069075 174499_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8643615-8643978(+) 100.0 100.0
Public Domain and Genome References
Ensembl F42A9.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB03166 Temporarily assigned gene name protein 137, isoform a [Caenorhabditis elegans] ref|NP_501476.1| F42A9.1a [Caenorhabditis elegans] 0.0
blast AAB03165 Temporarily assigned gene name protein 137, isoform b [Caenorhabditis elegans] ref|NP_501477.1| F42A9.1b [Caenorhabditis elegans] 0.0
blast CAE60006 Hypothetical protein CBG03505 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 3.1E-62
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.1E-11
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 8.3E-57
Pfam IPR001660 EMBL-EBI Sterile alpha motif SAM 2.2E-24
Sequence
Target Sequence 
>C. ELEGANS:174499_AT
ggaccaaattgtagacgccaggcgtccccttaccaaatttccccaaattcttccttaaaa
tttgcacatttgctcatttttcaatcagattttttaacattttagactattttcacggct
ttttttgaaattcaaaagtctatccgaaaactaatcaaaaattgaccaaactaaactatt
atatgcgataatttatatttcaaaacaaaaccgtttccaaatgtcacacacaatttgtcc
tcattaaacacatcacacacaactttcctcacgctccgcccactttcgaatctttcatgg
atttta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACCAAATTGTAGACGCCAGGCGT 273 523 34 Antisense
TTGTAGACGCCAGGCGTCCCCTTAC 247 707 42 Antisense
AATTCTTCCTTAAAATTTGCACATT 356 177 79 Antisense
CATTTTAGACTATTTTCACGGCTTT 591 215 131 Antisense
CAAAAGTCTATCCGAAAACTAATCA 516 187 166 Antisense
GACCAAACTAAACTATTATATGCGA 6 383 197 Antisense
AAACCGTTTCCAAATGTCACACACA 118 127 241 Antisense
CACACACAATTTGTCCTCATTAAAC 31 195 258 Antisense
GTCCTCATTAAACACATCACACACA 706 475 270 Antisense
TAAACACATCACACACAACTTTCCT 251 641 278 Antisense
ACTTTCGAATCTTTCATGGATTTTA 449 101 315 Antisense
Sequence Source Affymetrix Proprietary Database