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[174498_at]

Description C54523_rc /REP_DB=TREMBL Accession /GB=C54523 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk360b11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 16      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 20      6grp_total > 16      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1576
1 2 3 4 range
A 2151.3
(3)
P 		575.8
(-3)
P 		738.2
(-2.4)
P 		976.9
(-1.5)
P 		1576
B 2021.5
(3)
P 		996.5
(-3)
P 		1030.5
(-2.8)
P 		1062.5
(-2.6)
P 		1025
C 1931.2
(3)
P 		1156.4
(-2.4)
P 		1076
(-3)
P 		1077.8
(-3)
P 		855
avr 2034.7
(3) 		909.6
(-3) 		948.2
(-2.8) 		1039.1
(-2.3) 		1125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1529
1 2 3 4 5 6 range
A 1532.6
(3)
P 		1274.9
(1.2)
P 		813.8
(-2)
P 		671.4
(-3)
P 		984.8
(-0.8)
P 		936.8
(-1.2)
P 		861
B 2200.5
(3)
P 		1377.4
(-0.4)
P 		819.5
(-2.7)
P 		1308.3
(-0.7)
P 		735.4
(-3)
P 		849.4
(-2.5)
P 		1465
C 1682.3
(3)
P 		1562.4
(2.2)
P 		778.5
(-3)
P 		780.8
(-3)
P 		778.9
(-3)
P 		1096
(-0.9)
P 		904
avr 1805.1
(3) 		1404.9
(0.6) 		803.9
(-3) 		920.2
(-2.3) 		833
(-2.8) 		960.7
(-2.1) 		1001
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 658
1 2 3 4 range
A 783.3
(3)
P 		125
(-3)
P 		193
(-2.4)
P 		143
(-2.8)
P 		658
B 644.7
(3)
P 		246.1
(-2.7)
P 		238.8
(-2.8)
P 		226.4
(-3)
P 		418
C 639.3
(3)
P 		332.5
(-1)
P 		375
(-0.5)
P 		181.7
(-3)
P 		458
avr 689.1
(3) 		234.5
(-2.4) 		268.9
(-2) 		183.7
(-3) 		505

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 609
1 2 3 4 5 6 range
A 282.5
(-0.6)
P 		453.4
(3)
P 		177.2
(-2.9)
P 		170.3
(-3)
P 		297.2
(-0.3)
P 		228.1
(-1.8)
P 		283
B 765.7
(3)
P 		458.5
(-0.3)
P 		199
(-3)
P 		346.5
(-1.4)
P 		298.8
(-1.9)
P 		316.7
(-1.8)
P 		567
C 623.6
(3)
P 		476.7
(1.1)
P 		198.1
(-2.5)
P 		157
(-3)
P 		252
(-1.8)
P 		366.9
(-0.3)
P 		467
avr 557.3
(3) 		462.9
(1.5) 		191.4
(-3) 		224.6
(-2.5) 		282.7
(-1.5) 		303.9
(-1.2) 		366
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174498_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20590
Sequence Type Exemplar sequence
Representative Public ID C54523 NCBI
Target Description C54523_rc /REP_DB=TREMBL Accession /GB=C54523 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk360b11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063465(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063465 NCBI Caenorhabditis elegans LEThal family member (let-19) (let-19) mRNA, complete cds. 11/11 None
K08F8.6 ENSEMBL cdna:known chromosome:CEL140:II:8770995:8781939:1 gene:K08F8.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8781563-8781860(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol let-19
Ensembl K08F8.6
Entrez Gene 174402 Entrez gene
SwissProt Q93442 EMBL-EBI
WormBase CE37536 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92667 Hypothetical protein K08F8.6 [Caenorhabditis elegans] emb|CAA91289.2| Hypothetical protein K08F8.6 [Caenorhabditis elegans] ref|NP_495866.2| LEThal family member (let-19) [Caenorhabditis elegans] dbj|BAE16563.1| Mediator complex subunit Med13 [Caenorhabditis elegans] 0.0
blast CAE59732 Hypothetical protein CBG03168 [Caenorhabditis briggsae] 0.0
blast CAF99193 unnamed protein product [Tetraodon nigroviridis] 2.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR009401 EMBL-EBI TRAP240 9.6E-12
Sequence
Target Sequence 
>C. ELEGANS:174498_AT
tttcaaacctttttcaatatattgttaacctgttatcaaatatctcaatctctagaacgc
taaccagtcgtatttccttgtttgttcgttcatctagaatcacagaatagacgtgtgttt
gtgggtttcagctctctttccctgtccccttctcaatattcgggtttcaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTCAAACCTTTTTCAATATATTGT 405 671 41 Antisense
TCAATATATTGTTAACCTGTTATCA 1 635 54 Antisense
AATATCTCAATCTCTAGAACGCTAA 699 173 79 Antisense
CAATCTCTAGAACGCTAACCAGTCG 455 175 86 Antisense
GAACGCTAACCAGTCGTATTTCCTT 229 347 95 Antisense
GCTAACCAGTCGTATTTCCTTGTTT 680 301 99 Antisense
CAGTCGTATTTCCTTGTTTGTTCGT 613 207 105 Antisense
TTTCCTTGTTTGTTCGTTCATCTAG 393 669 113 Antisense
TTGTTTGTTCGTTCATCTAGAATCA 429 705 118 Antisense
CTGTCCCCTTCTCAATATTCGGGTT 520 231 182 Antisense
CCTTCTCAATATTCGGGTTTCAATA 209 267 188 Antisense
Sequence Source Affymetrix Proprietary Database