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[174478_s_at]

Description C32377_rc /REP_DB=TREMBL Accession /GB=C32377 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk329h10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 02      4grp_highest_8_8 > 26      4grp_high_8_8 > 63      6grp_highest_nonorm > 67      6grp_total > 45      4grp_total > 59      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1747
1 2 3 4 range
A 2411.6
(0.3)
P 		2374.2
(0.1)
P 		2996.4
(3)
P 		1710.7
(-3)
P 		1286
B 2649.6
(1)
P 		2065.4
(-1.8)
P 		3078.5
(3)
P 		1813.9
(-3)
P 		1265
C 2002.2
(-1)
P 		2428.3
(0.4)
P 		3181.5
(3)
P 		1434.3
(-3)
P 		1747
avr 2354.5
(-0.1) 		2289.3
(-0.3) 		3085.5
(3) 		1653
(-3) 		1433

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1083
1 2 3 4 5 6 range
A 1898.6
(2.3)
P 		1794.1
(1.4)
P 		1981.6
(3)
P 		1920.4
(2.5)
P 		1279.9
(-3)
P 		1777.6
(1.3)
P 		702
B 2363.1
(3)
P 		1963.1
(0.4)
P 		1782.8
(-0.8)
P 		1585.3
(-2.1)
P 		1448.2
(-3)
P 		1799.2
(-0.7)
P 		915
C 1974.5
(1)
P 		1837.3
(0)
P 		2265.5
(3)
P 		1629.1
(-1.4)
P 		1401.4
(-3)
P 		1931.7
(0.7)
P 		864
avr 2078.7
(3) 		1864.8
(1.2) 		2010
(2.4) 		1711.6
(-0.1) 		1376.5
(-3) 		1836.2
(0.9) 		702
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 867
1 2 3 4 range
A 878.1
(3)
P 		515.4
(-0.5)
P 		783.3
(2.1)
P 		250.4
(-3)
P 		628
B 844.9
(3)
P 		510.1
(-1.4)
P 		713.3
(1.3)
P 		386.5
(-3)
P 		458
C 662.8
(-0.1)
P 		698.1
(0.2)
P 		1108.9
(3)
P 		241.8
(-3)
P 		867
avr 795.3
(2.2) 		574.5
(-0.1) 		868.5
(3) 		292.9
(-3) 		576

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 495
1 2 3 4 5 6 range
A 350
(-3)
P 		638.1
(3)
P 		431.5
(-1.3)
P 		487.1
(-0.1)
P 		386.2
(-2.2)
P 		432.8
(-1.3)
P 		288
B 822.2
(3)
P 		653.4
(0.5)
P 		432.9
(-2.8)
P 		419.9
(-3)
P 		588.4
(-0.5)
P 		670.9
(0.7)
P 		402
C 731.9
(3)
P 		560.6
(0.5)
P 		576.6
(0.7)
P 		327.6
(-3)
P 		453.5
(-1.1)
P 		646.6
(1.7)
P 		404
avr 634.7
(3) 		617.4
(2.5) 		480.3
(-1.2) 		411.5
(-3) 		476
(-1.3) 		583.4
(1.6) 		223
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174478_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21012
Sequence Type Exemplar sequence
Representative Public ID C32377 NCBI
Target Description C32377_rc /REP_DB=TREMBL Accession /GB=C32377 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk329h10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066143(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066143 NCBI Caenorhabditis elegans ZK418.5 (ZK418.5) mRNA, complete cds. 11/11 A 
SNAP00000003342 ENSEMBL cdna:SNAP chromosome:CEL140:III:7072759:7073759:-1 11/11 None
GENEFINDER00000003352 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7072759:7073759:-1 11/11 None
ZK418.5 ENSEMBL cdna:known chromosome:CEL140:III:7072654:7073771:-1 gene:ZK418.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7072661-7073016(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK418.5
Entrez Gene 175987 Entrez gene
SwissProt Q23483 EMBL-EBI
WormBase CE28189 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262426_S_AT hypothetical protein at
ATGENOME1:15466_AT expressed protein at
CANINE:1583445_S_AT similar to seven transmembrane domain protein cfa
CANINE_2:CFA.4869.1.A1_S_AT similar to seven transmembrane domain protein cfa
DROSGENOME1:143800_AT dm
DROSOPHILA_2:1641285_AT dm
HG-U133_PLUS_2:202475_AT seven transmembrane domain protein hs
HG-U133A:202475_AT seven transmembrane domain protein hs
HG-FOCUS:202475_AT seven transmembrane domain protein hs
HG-U133A_2:202475_AT seven transmembrane domain protein hs
HU35KSUBA:RC_AA485358_AT seven transmembrane domain protein hs
HU35KSUBA:AA296994_S_AT seven transmembrane domain protein hs
U133_X3P:G5453781_3P_AT seven transmembrane domain protein hs
HG-U95AV2:39088_AT seven transmembrane domain protein hs
MOE430A:1428465_AT RIKEN cDNA 5033425B17 gene mm
MOUSE430A_2:1428465_AT RIKEN cDNA 5033425B17 gene mm
MOUSE430_2:1428465_AT RIKEN cDNA 5033425B17 gene mm
MG-U74AV2:160235_AT RIKEN cDNA 5033425B17 gene mm
MG-U74CV2:171143_R_AT RIKEN cDNA 5033425B17 gene (5033425B17Rik), mRNA mm
MU19KSUBB:TC30795_I_AT RIKEN cDNA 5033425B17 gene (5033425B17Rik), mRNA mm
MU19KSUBB:TC30795_S_AT RIKEN cDNA 5033425B17 gene (5033425B17Rik), mRNA mm
MU19KSUBB:TC30796_S_AT RIKEN cDNA 5033425B17 gene (5033425B17Rik), mRNA mm
MU19KSUBC:TC39439_AT RIKEN cDNA 5033425B17 gene (5033425B17Rik), mRNA mm
MU19KSUBC:TC39439_G_AT RIKEN cDNA 5033425B17 gene (5033425B17Rik), mRNA mm
RG-U34C:RC_AI228642_AT similar to seven transmembrane domain protein (predicted) rn
RAE230A:1372230_AT similar to seven transmembrane domain protein (predicted) rn
RAT230_2:1372230_AT similar to seven transmembrane domain protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA50689 Hypothetical protein ZK418.5 [Caenorhabditis elegans] ref|NP_498544.1| ZK418.5 [Caenorhabditis elegans] 1.0E-126
blast CAE70075 Hypothetical protein CBG16516 [Caenorhabditis briggsae] 1.0E-113
blast NP_081491 RIKEN cDNA 5033425B17 [Mus musculus] dbj|BAB25453.1| unnamed protein product [Mus musculus] 1.0E-45
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498544.1 7 4-26,38-60,75-94,101-123,138-157,164-183,193-215
ZK418.5 7 4-26,38-60,75-94,101-123,138-157,164-183,193-215
SNAP00000003342 7 4-26,38-60,75-94,101-123,138-157,164-183,193-215
GENEFINDER00000003352 7 4-26,38-60,75-94,101-123,138-157,164-183,193-215
Sequence
Target Sequence 
>C. ELEGANS:174478_S_AT
acccgcactccaaacaagttcttggtgtcaccgatccttgcgatgtgtgtcttctcaaca
ttcgtttatcaaaccgttcaacacacattctctctctacggatggtctctcttggcattc
cgctttgcctactccatcgcaacagcaattctcaccgtcgttgtctattcggcaaaccgg
acagcgtccacaagaaagaacgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCCGCACTCCAAACAAGTTCTTGG 698 89 16 Antisense
ACTCCAAACAAGTTCTTGGTGTCAC 416 99 22 Antisense
CTACTCCATCGCAACAGCAATTCTC 118 227 144 Antisense
TCCATCGCAACAGCAATTCTCACCG 438 589 148 Antisense
CAACAGCAATTCTCACCGTCGTTGT 669 187 155 Antisense
TCACCGTCGTTGTCTATTCGGCAAA 120 625 167 Antisense
GTCGTTGTCTATTCGGCAAACCGGA 276 473 172 Antisense
GTCTATTCGGCAAACCGGACAGCGT 641 467 178 Antisense
TTCGGCAAACCGGACAGCGTCCACA 29 695 183 Antisense
GCAAACCGGACAGCGTCCACAAGAA 632 315 187 Antisense
GACAGCGTCCACAAGAAAGAACGAG 329 367 195 Antisense
Sequence Source Affymetrix Proprietary Database