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[174469_at]

Description CEC3473_rc /REP_DB=TREMBL Accession /GB=C10347 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk144e3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 18      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 20      6grp_total > 28      4grp_total > 0      6grp_highest_sub > Cu      6grp_cgc > 02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 560
1 2 3 4 range
A 635.1
(-1.5)
P 		541.8
(-3)
P 		805.4
(1.1)
P 		925.4
(3)
P 		384
B 726.8
(-1.2)
P 		726.4
(-1.2)
P 		604.5
(-3)
P 		1001.9
(3)
P 		397
C 825.2
(-1.9)
P 		766.3
(-3)
P 		1101.6
(3)
P 		881.8
(-0.9)
P 		335
avr 729
(-1.8) 		678.2
(-3) 		837.2
(0.7) 		936.4
(3) 		258

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1897
1 2 3 4 5 6 range
A 1398.7
(3)
P 		1166
(1.2)
P 		774.1
(-1.9)
P 		640.7
(-3)
P 		1116.4
(0.8)
P 		693.9
(-2.6)
P 		758
B 1444.7
(3)
P 		1008.9
(0)
P 		570.6
(-3)
P 		897.5
(-0.8)
P 		786.8
(-1.5)
P 		905.9
(-0.7)
P 		874
C 2398.9
(3)
P 		1109.7
(-1.1)
P 		929.4
(-1.6)
P 		501.5
(-3)
P 		1155.3
(-0.9)
P 		813.7
(-2)
P 		1897
avr 1747.4
(3) 		1094.9
(-0.7) 		758
(-2.6) 		679.9
(-3) 		1019.5
(-1.1) 		804.5
(-2.3) 		1068
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 266
1 2 3 4 range
A 231.2
(3)
P 		117.6
(-3)
P 		210.5
(1.9)
P 		135.5
(-2.1)
P 		114
B 231.8
(3)
P 		179.4
(-0.4)
P 		140.1
(-3)
P 		213.5
(1.8)
P 		92
C 273.2
(0.2)
P 		220.3
(-1.2)
P 		384
(3)
P 		148.7
(-3)
P 		235
avr 245.4
(3) 		172.4
(-2.5) 		244.9
(3) 		165.9
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 788
1 2 3 4 5 6 range
A 257.8
(-0.7)
P 		414.7
(3)
P 		168.6
(-2.9)
P 		162.5
(-3)
P 		336.9
(1.1)
P 		169
(-2.8)
P 		252
B 502.7
(3)
P 		335.8
(0.2)
P 		138.6
(-3)
P 		237.7
(-1.4)
P 		319.7
(-0)
P 		337.8
(0.3)
P 		364
C 889.2
(3)
P 		338.6
(-1.2)
P 		236.6
(-2)
P 		100.9
(-3)
P 		373.8
(-0.9)
P 		272.4
(-1.7)
P 		788
avr 549.9
(3) 		363
(0.1) 		181.3
(-2.8) 		167
(-3) 		343.5
(-0.2) 		259.7
(-1.5) 		383
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174469_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21701
Sequence Type Exemplar sequence
Representative Public ID CEC3473 NCBI
Target Description CEC3473_rc /REP_DB=TREMBL Accession /GB=C10347 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk144e3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171472(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171472 NCBI Caenorhabditis elegans ALdehyde deHydrogenase family member (alh-4) (alh-4) mRNA, complete cds. 11/11 None
T05H4.13b ENSEMBL cdna:known chromosome:CEL140:V:6429816:6432506:-1 gene:T05H4.13 11/11 A 
T05H4.13c.2 ENSEMBL cdna:known chromosome:CEL140:V:6429817:6432510:-1 gene:T05H4.13 11/11 None
T05H4.13c.3 ENSEMBL cdna:known chromosome:CEL140:V:6429817:6432510:-1 gene:T05H4.13 11/11 None
T05H4.13c.1 ENSEMBL cdna:known chromosome:CEL140:V:6429817:6432508:-1 gene:T05H4.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179026 3 1 NM_171472 174469_at (11), 190762_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6429884-6430098(-) 100.0 100.0
Public Domain and Genome References
Ensembl T05H4.13
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741554 ALdehyde deHydrogenase family member (alh-4) [Caenorhabditis elegans] gb|AAM45357.1| Aldehyde dehydrogenase protein 4, isoform b [Caenorhabditis elegans] 0.0
blast NP_504634 ALdehyde deHydrogenase family member (alh-4) [Caenorhabditis elegans] gb|AAO26003.1| Aldehyde dehydrogenase protein 4, isoform c [Caenorhabditis elegans] 0.0
blast NP_741553 ALdehyde deHydrogenase family member (alh-4) [Caenorhabditis elegans] gb|AAB66022.2| Aldehyde dehydrogenase protein 4, isoform a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002086 EMBL-EBI Aldehyde dehydrogenase 2.2E-35
Pfam IPR002086 EMBL-EBI Aldehyde dehydrogenase 2.4E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741554.1 1 469-491
T05H4.13b 1 469-491
T05H4.13c.2 1 463-485
T05H4.13c.3 1 463-485
T05H4.13c.1 1 463-485
Sequence
Target Sequence 
>C. ELEGANS:174469_AT
tttttttcacaaattcctagtcaattttttcaccggatcttctaatcattccataaactc
tagtccataaccaattcccgcatgaaaagtgtccccaaatattaaaaattcgtgtagttt
caatttaaagcaataattttattaattttcattcccgatattttcagaaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTTTTCACAAATTCCTAGTCAAT 660 673 17 Antisense
TAGTCAATTTTTTCACCGGATCTTC 529 651 34 Antisense
TTCACCGGATCTTCTAATCATTCCA 531 687 45 Antisense
TAATCATTCCATAAACTCTAGTCCA 284 635 59 Antisense
TAAACTCTAGTCCATAACCAATTCC 504 639 70 Antisense
TCCATAACCAATTCCCGCATGAAAA 691 589 80 Antisense
TTCCCGCATGAAAAGTGTCCCCAAA 645 695 91 Antisense
TGTCCCCAAATATTAAAAATTCGTG 277 555 106 Antisense
ATTCGTGTAGTTTCAATTTAAAGCA 369 5 124 Antisense
TAATTTTCATTCCCGATATTTTCAG 687 633 159 Antisense
TTCATTCCCGATATTTTCAGAAATA 442 689 164 Antisense
Sequence Source Affymetrix Proprietary Database