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[174459_at]

Description CEC3394_rc /REP_DB=TREMBL Accession /GB=C11339 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk171a5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 92      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 49      6grp_total > 83      4grp_total > 11      6grp_highest_sub > 04_05      6grp_cgc > 87      6grp_high1_sub > 04_05      6grp_cgc_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 340
1 2 3 4 range
A 127.9
(-1.6)
P 		117.5
(-1.8)
P 		50.3
(-3)
P 		390.3
(3)
P 		340
B 109.4
(-2.3)
P 		79.5
(-3)
P 		102.3
(-2.5)
P 		339.8
(3)
P 		260
C 86.4
(-2.6)
P 		140.8
(-1.6)
P 		66.7
(-3)
P 		380.3
(3)
P 		314
avr 107.9
(-2.3) 		112.6
(-2.2) 		73.1
(-3) 		370.1
(3) 		297

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13758
1 2 3 4 5 6 range
A 49.2
(-3)
P 		67.5
(-3)
P 		245.8
(-2.9)
P 		10948.8
(3)
P 		10648.9
(2.8)
P 		3440.7
(-1.1)
P 		10900
B 66.2
(-3)
P 		39.5
(-3)
P 		160.6
(-2.9)
P 		8899.2
(1.9)
P 		10850.6
(3)
P 		3405.5
(-1.1)
P 		10811
C 40.1
(-3)
P 		68.9
(-3)
P 		273.7
(-2.9)
P 		8302.3
(0.6)
P 		13797.9
(3)
P 		2849.9
(-1.8)
P 		13758
avr 51.8
(-3) 		58.6
(-3) 		226.7
(-2.9) 		9383.4
(1.8) 		11765.8
(3) 		3232
(-1.4) 		11714
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 46.6
(1.6)
P 		25.5
(-1.3)
P 		13.1
(-3)
P 		57.1
(3)
P 		44
B 34.9
(-1.3)
P 		19.6
(-3)
P 		23.7
(-2.5)
P 		72.4
(3)
P 		53
C 28.6
(-2.2)
P 		40.5
(-0.5)
P 		23.2
(-3)
P 		64.1
(3)
P 		41
avr 36.7
(-0.7) 		28.5
(-1.9) 		20
(-3) 		64.5
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4456
1 2 3 4 5 6 range
A 9.1
(-3)
P 		24
(-3)
P 		53.5
(-2.9)
P 		2776.9
(2.2)
P 		3213.4
(3)
P 		837.7
(-1.4)
P 		3204
B 23.1
(-3)
P 		13.1
(-3)
P 		39
(-3)
P 		2357.1
(0.2)
P 		4408.4
(3)
P 		1269.9
(-1.3)
P 		4395
C 14.9
(-3)
P 		21
(-3)
P 		69.7
(-2.9)
P 		1669.6
(-0.8)
P 		4464.8
(3)
P 		954
(-1.7)
P 		4450
avr 15.7
(-3) 		19.4
(-3) 		54.1
(-2.9) 		2267.9
(0.4) 		4028.9
(3) 		1020.5
(-1.5) 		4013
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174459_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21008
Sequence Type Exemplar sequence
Representative Public ID CEC3394 NCBI
Target Description CEC3394_rc /REP_DB=TREMBL Accession /GB=C11339 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk171a5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027854
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
ZK643.8 ENSEMBL cdna:known chromosome:CEL140:III:8962200:8965543:1 gene:ZK643.8 A 
NM_001027854 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-25) (grl-25) mRNA, complete cds. None
SNAP00000011222 ENSEMBL cdna:SNAP chromosome:CEL140:III:8962200:8963384:1 None
GENEFINDER00000011235 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8962200:8963328:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8963261-8963541(+) 95.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-25
Ensembl ZK643.8
Entrez Gene 176265 Entrez gene
SwissProt Q868B4 EMBL-EBI
WormBase CE35164 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAD88219 Hypothetical protein ZK643.8 [Caenorhabditis elegans] emb|CAD88221.2| Hypothetical protein ZK643.8 [Caenorhabditis elegans] ref|NP_001023025.1| GRound-Like (grd related) family member (grl-25) [Caenorhabditis elegans] 1.0E-55
blast CAE62711 Hypothetical protein CBG06865 [Caenorhabditis briggsae] 1.0E-50
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023025.1 1 7-29
ZK643.8 1 7-29
SNAP00000011222 1 7-29
GENEFINDER00000011235 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:174459_AT
ctccagctccagtgtcgagcggaggaggatactctgaacaagcaagtggtggttcttctt
acccgtcacgcttcagattctcgcgcttcttcaacaaggcataatttaagtaccatcaca
attcccaatgatgccccccgttatgatattgttgtgagaaatgagagtgactgtagcagt
caattctcaacaagaagtgtctagtattgtctcttttccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCAGCTCCAGTGTCGAGCGGAGG 85 235 28 Antisense
GTCGAGCGGAGGAGGATACTCTGAA 693 473 41 Antisense
ATACTCTGAACAAGCAAGTGGTGGT 371 23 56 Antisense
AGCAAGTGGTGGTTCTTCTTACCCG 407 75 68 Antisense
TCTCGCGCTTCTTCAACAAGGCATA 367 609 106 Antisense
CAAGGCATAATTTAAGTACCATCAC 613 183 122 Antisense
TTTAAGTACCATCACAATTCCCAAT 572 667 132 Antisense
CATCACAATTCCCAATGATGCCCCC 279 213 141 Antisense
TGCCCCCCGTTATGATATTGTTGTG 703 583 159 Antisense
GACTGTAGCAGTCAATTCTCAACAA 525 375 196 Antisense
AGTGTCTAGTATTGTCTCTTTTCCC 642 59 223 Antisense
Sequence Source Affymetrix Proprietary Database