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[174449_at]

Description C57971_rc /REP_DB=TREMBL Accession /GB=C57971 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk310h11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 13      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 12      6grp_total > 13      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1988
1 2 3 4 range
A 2929.3
(3)
P 		1646.5
(-1)
P 		1806
(-0.5)
P 		1011.7
(-3)
P 		1918
B 2267.4
(3)
P 		1653.1
(-0.6)
P 		1345.1
(-2.3)
P 		1232.1
(-3)
P 		1035
C 2319.9
(3)
P 		1427.4
(-0.9)
P 		1479.2
(-0.7)
P 		941.7
(-3)
P 		1378
avr 2505.5
(3) 		1575.7
(-0.9) 		1543.4
(-1) 		1061.8
(-3) 		1444

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1037
1 2 3 4 5 6 range
A 2444.5
(3)
P 		2307.6
(2.1)
P 		1945.1
(-0.4)
P 		1948.2
(-0.3)
P 		1979.2
(-0.1)
P 		1553.6
(-3)
P 		891
B 2248.6
(2.4)
P 		2336.5
(3)
P 		1745.3
(-0.9)
P 		2004.1
(0.8)
P 		1429.8
(-3)
P 		1495.5
(-2.6)
P 		907
C 2197.7
(1.8)
P 		2302.1
(2.4)
P 		2403.4
(3)
P 		1962.3
(0.3)
P 		1582.1
(-1.9)
P 		1407.4
(-3)
P 		996
avr 2296.9
(2.9) 		2315.4
(3) 		2031.3
(0.9) 		1971.5
(0.5) 		1663.7
(-1.7) 		1485.5
(-3) 		830
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 918
1 2 3 4 range
A 1066.5
(3)
P 		357.4
(-1.6)
P 		472.1
(-0.9)
P 		148.1
(-3)
P 		918
B 723.1
(3)
P 		408.3
(-1.1)
P 		311.7
(-2.4)
P 		262.6
(-3)
P 		461
C 768
(3)
P 		410.4
(-0.5)
P 		515.5
(0.5)
P 		158.8
(-3)
P 		609
avr 852.5
(3) 		392
(-1.2) 		433.1
(-0.8) 		189.8
(-3) 		663

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 442
1 2 3 4 5 6 range
A 450.6
(-2)
P 		820.7
(3)
P 		423.6
(-2.4)
P 		494.1
(-1.4)
P 		597.2
(-0)
P 		378.3
(-3)
P 		442
B 782.4
(3)
P 		777.7
(2.9)
P 		423.8
(-3)
P 		530.8
(-1.2)
P 		580.9
(-0.4)
P 		557.7
(-0.8)
P 		359
C 814.6
(3)
P 		702.4
(1.4)
P 		611.7
(0.1)
P 		394.6
(-3)
P 		512
(-1.3)
P 		471.1
(-1.9)
P 		420
avr 682.5
(1.3) 		766.9
(3) 		486.4
(-2.6) 		473.2
(-2.9) 		563.4
(-1.1) 		469
(-3) 		298
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174449_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21156
Sequence Type Exemplar sequence
Representative Public ID C57971 NCBI
Target Description C57971_rc /REP_DB=TREMBL Accession /GB=C57971 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk310h11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066729(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066729 NCBI Caenorhabditis elegans F58A4.7a (AP-4) mRNA, complete cds. 11/11 None
F58A4.7a.2 ENSEMBL cdna:known chromosome:CEL140:III:9622040:9626433:1 gene:F58A4.7 11/11 None
F58A4.7a.1 ENSEMBL cdna:known chromosome:CEL140:III:9622049:9626445:1 gene:F58A4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176360 4 1 NM_001027455 192781_s_at (11)
NM_066728 192781_s_at (11)
NM_066729 174449_at (11), 192781_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9626083-9626363(+) 100.0 100.0
Public Domain and Genome References
Ensembl F58A4.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA80167 Hypothetical protein F58A4.7a [Caenorhabditis elegans] ref|NP_499130.2| F58A4.7a [Caenorhabditis elegans] 1.0E-178
blast CAA80170 Hypothetical protein F58A4.7b [Caenorhabditis elegans] ref|NP_499129.2| F58A4.7b [Caenorhabditis elegans] sp|P34474|YMH7_CAEEL Hypothetical protein F58A4.7 in chromosome III 1.0E-177
blast CAD90178 Hypothetical protein F58A4.7c [Caenorhabditis elegans] ref|NP_001022626.1| F58A4.7c [Caenorhabditis elegans] 1.0E-177
Protein Domains
Database ID Description E-Value
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 1.4E-16
Sequence
Target Sequence 
>C. ELEGANS:174449_AT
gattgtggtcttcttgtcatttttcttctaatcaatctagcttccttatttcccgcgctc
tcacggacctatccattgtttcgttctccccaccgataactttttgttttttcattttcc
tttttttccagtcttgttcatctccagacactgccccgttacttttacacaaatcgtgtc
gatttcctctttttttcatttttcacccggtggttttgttgaccgtatgtgcagcttgaa
acactttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGTGGTCTTCTTGTCATTTTTC 373 435 14 Antisense
TTGTCATTTTTCTTCTAATCAATCT 144 709 27 Antisense
TAATCAATCTAGCTTCCTTATTTCC 618 635 42 Antisense
TCTCACGGACCTATCCATTGTTTCG 520 613 72 Antisense
ACGGACCTATCCATTGTTTCGTTCT 140 95 76 Antisense
TTTCCAGTCTTGTTCATCTCCAGAC 580 669 138 Antisense
CAGTCTTGTTCATCTCCAGACACTG 280 207 142 Antisense
TGCCCCGTTACTTTTACACAAATCG 226 585 165 Antisense
CAAATCGTGTCGATTTCCTCTTTTT 681 191 183 Antisense
GTTTTGTTGACCGTATGTGCAGCTT 633 447 226 Antisense
GTATGTGCAGCTTGAAACACTTTCA 19 457 238 Antisense
Sequence Source Affymetrix Proprietary Database