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[174444_at] calcineurin B

Description C38662_rc /REP_DB=TREMBL Accession /GB=C38662 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk496e12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 12      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 22      6grp_total > 23      4grp_total > 62      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 875
1 2 3 4 range
A 2134.8
(3)
P 		1499.9
(-1.4)
P 		1661.3
(-0.2)
P 		1259.7
(-3)
P 		875
B 2056.9
(3)
P 		1755.8
(0.6)
P 		1628.8
(-0.4)
P 		1306.6
(-3)
P 		750
C 1991.7
(3)
P 		1824.1
(1.6)
P 		1797.1
(1.4)
P 		1281.3
(-3)
P 		710
avr 2061.1
(3) 		1693.3
(0.2) 		1695.7
(0.2) 		1282.5
(-3) 		779

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1030
1 2 3 4 5 6 range
A 1854.6
(3)
P 		1486.5
(0.4)
P 		1644.8
(1.5)
P 		1297.3
(-1)
P 		1673.1
(1.7)
P 		1019.2
(-3)
P 		835
B 2049.4
(3)
P 		1798.4
(0.5)
P 		1932.3
(1.8)
P 		1640.5
(-1)
P 		1523.6
(-2.2)
P 		1443.6
(-3)
P 		606
C 1421.4
(-0.7)
P 		1867.3
(3)
P 		1448.5
(-0.4)
P 		1684.4
(1.5)
P 		1134.6
(-3)
P 		1569.7
(0.6)
P 		733
avr 1775.1
(3) 		1717.4
(2.2) 		1675.2
(1.6) 		1540.7
(-0.3) 		1443.8
(-1.6) 		1344.2
(-3) 		431
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 593
1 2 3 4 range
A 777.3
(3)
P 		325.6
(-1.6)
P 		434.3
(-0.5)
P 		184.4
(-3)
P 		593
B 655.9
(3)
P 		433.6
(-0.5)
P 		377.4
(-1.4)
P 		278.4
(-3)
P 		378
C 659.3
(3)
P 		524.4
(1.2)
P 		626.3
(2.6)
P 		216
(-3)
P 		443
avr 697.5
(3) 		427.9
(-0.4) 		479.3
(0.2) 		226.3
(-3) 		471

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 465
1 2 3 4 5 6 range
A 341.9
(-1)
P 		528.7
(3)
P 		358.2
(-0.6)
P 		329
(-1.3)
P 		504.9
(2.5)
P 		248.2
(-3)
P 		281
B 713.1
(3)
P 		598.6
(0.5)
P 		469.2
(-2.3)
P 		434.5
(-3)
P 		619
(1)
P 		538.3
(-0.8)
P 		279
C 526.9
(1.9)
P 		569.8
(3)
P 		368.7
(-2.2)
P 		338.7
(-3)
P 		367.1
(-2.3)
P 		525.5
(1.8)
P 		231
avr 527.3
(1.8) 		565.7
(3) 		398.7
(-2.1) 		367.4
(-3) 		497
(0.9) 		437.3
(-0.9) 		198
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174444_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21946
Sequence Type Exemplar sequence
Representative Public ID C38662 NCBI
Target Description C38662_rc /REP_DB=TREMBL Accession /GB=C38662 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk496e12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073484(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073484 NCBI Caenorhabditis elegans CalciNeurin B family member (cnb-1) (cnb-1) mRNA, complete cds. 11/11 None
F55C10.1 ENSEMBL cdna:known chromosome:CEL140:V:11378379:11380648:1 gene:F55C10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11380024-11380304(+) 100.0 100.0
Public Domain and Genome References
Gene Title calcineurin B
Gene Symbol cnb-1
Ensembl F55C10.1
Entrez Gene 179572 Entrez gene
SwissProt Q20804 EMBL-EBI
WormBase CE32679 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604857_AT similar to Calcineurin B subunit isoform 1 (Protein phosphatase 2B regulatory subunit 1) (Protein phosphatase 3 regulatory subunit B alpha isoform 1) cfa
CANINE_2:CFA.10339.1.S1_AT similar to Calcineurin B subunit isoform 1 (Protein phosphatase 2B regulatory subunit 1) (Protein phosphatase 3 regulatory subunit B alpha isoform 1) cfa
CANINE_2:CFAAFFX.5810.1.S1_AT similar to Calcineurin B subunit isoform 1 (Protein phosphatase 2B regulatory subunit 1) (Protein phosphatase 3 regulatory subunit B alpha isoform 1) cfa
CANINE_2:CFAAFFX.5810.1.S1_S_AT similar to Calcineurin B subunit isoform 1 (Protein phosphatase 2B regulatory subunit 1) (Protein phosphatase 3 regulatory subunit B alpha isoform 1) cfa
DROSOPHILA_2:1627067_AT Calcineurin B2 dm
DROSGENOME1:143830_AT Calcineurin B2 dm
CHICKEN:GGA.3833.1.S1_S_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) gga
CHICKEN:GGA.3833.1.S2_S_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) gga
HG-U133A:204507_S_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U133_PLUS_2:204507_S_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U133A_2:204507_S_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U95AV2:40513_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HU35KSUBB:RC_AA018907_S_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HUGENEFL:M30773_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
U133_X3P:G4506024_3P_A_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
U133_X3P:HS.278540.0.A2_3P_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U133A_2:204506_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-FOCUS:204506_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U133_PLUS_2:204506_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U133A:204506_AT protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U133_PLUS_2:1565810_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U133_PLUS_2:241786_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U133B:241786_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U95B:57841_F_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U95B:57848_R_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HG-U95E:80338_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HU35KSUBA:RC_AA452857_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
HU35KSUBC:RC_T86603_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
U133_X3P:HS.302447.0.A1_3P_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
U133_X3P:HS2.375807.1.A1_3P_AT Protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) hs
RG-U34A:D14425_S_AT protein phospatase 3, regulatory subunit B, alpha isoform,type 1 rn
RG-U34A:D14568_AT protein phospatase 3, regulatory subunit B, alpha isoform,type 1 rn
RAE230A:1372982_AT protein phospatase 3, regulatory subunit B, alpha isoform,type 1 rn
RAT230_2:1372982_AT protein phospatase 3, regulatory subunit B, alpha isoform,type 1 rn
RAE230A:1369152_AT protein phospatase 3, regulatory subunit B, alpha isoform,type 1 rn
RAT230_2:1369152_AT protein phospatase 3, regulatory subunit B, alpha isoform,type 1 rn
RAE230B:1391213_AT protein phospatase 3, regulatory subunit B, alpha isoform,type 1 rn
RAT230_2:1391213_AT protein phospatase 3, regulatory subunit B, alpha isoform,type 1 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98489 Hypothetical protein F55C10.1 [Caenorhabditis elegans] ref|NP_505885.2| CalciNeurin B family member (cnb-1) [Caenorhabditis elegans] gb|AAO33925.1| CNB-1 [Caenorhabditis elegans] emb|CAE75232.1| Hypothetical protein CBG23183 [Caenorhabditis briggsae] 9.0E-94
blast AAK83039 calcineurin B [Bombyx mori] 1.0E-75
blast EAA44557 ENSANGP00000024230 [Anopheles gambiae str. PEST] ref|XP_562039.1| ENSANGP00000024230 [Anopheles gambiae str. PEST] 3.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.5E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.9E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 6.6E-7
Sequence
Target Sequence 
>C. ELEGANS:174444_AT
gactttctagtccattcaatttttcatctgaatttagccgtttttccgatatgtccccgt
agctgtcacttgtacatgtttccccaaccgatttcccctttttctctttctggctctctt
gctctttgatctatgctgaaaaaactatatttataaaataaggtttgatttaaatatatt
cttcgaaaaaattcccattcgtcaatctagtcaatgtttcccctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTTCTAGTCCATTCAATTTTTC 493 371 17 Antisense
TTTTCATCTGAATTTAGCCGTTTTT 254 675 37 Antisense
GAATTTAGCCGTTTTTCCGATATGT 197 325 46 Antisense
CCCGTAGCTGTCACTTGTACATGTT 241 261 72 Antisense
TAGCTGTCACTTGTACATGTTTCCC 505 651 76 Antisense
CTCTCTTGCTCTTTGATCTATGCTG 450 231 130 Antisense
CTTGCTCTTTGATCTATGCTGAAAA 346 219 134 Antisense
GGTTTGATTTAAATATATTCTTCGA 702 505 178 Antisense
TATTCTTCGAAAAAATTCCCATTCG 676 659 193 Antisense
AATTCCCATTCGTCAATCTAGTCAA 708 175 206 Antisense
GTCAATCTAGTCAATGTTTCCCCTT 446 463 217 Antisense
Sequence Source Affymetrix Proprietary Database