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[174434_at]

Description CEK003D8R_rc /REP_DB=TREMBL Accession /GB=D27758 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk3d8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 48      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 84      6grp_total > 38      4grp_total > 39      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 426
1 2 3 4 range
A 712.2
(3)
P 		513.6
(-1.9)
P 		468.6
(-3)
P 		625.6
(0.9)
P 		244
B 820.1
(3)
P 		567.2
(-1.4)
P 		477.7
(-3)
P 		689.2
(0.7)
P 		342
C 894.2
(3)
P 		502.9
(-3)
P 		648.7
(-0.8)
P 		573
(-1.9)
P 		391
avr 808.8
(3) 		527.9
(-3) 		531.7
(-2.9) 		629.3
(-0.8) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 473
1 2 3 4 5 6 range
A 564.4
(-0.9)
P 		782.4
(3)
P 		538.6
(-1.3)
P 		443.2
(-3)
P 		542
(-1.3)
P 		505.6
(-1.9)
P 		339
B 810.8
(3)
P 		621.8
(-0.2)
P 		456.7
(-3)
P 		563.3
(-1.2)
P 		495.1
(-2.3)
P 		787.9
(2.6)
P 		354
C 912.2
(3)
P 		563.2
(-1.4)
P 		536.5
(-1.8)
P 		439.1
(-3)
P 		706.3
(0.4)
P 		853.9
(2.3)
P 		473
avr 762.5
(3) 		655.8
(0.7) 		510.6
(-2.4) 		481.9
(-3) 		581.1
(-0.9) 		715.8
(2) 		281
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 259.3
(3)
P 		111.5
(-2.3)
P 		122.5
(-1.9)
P 		91.6
(-3)
P 		168
B 261.5
(3)
P 		140.1
(-1.8)
P 		110.7
(-3)
P 		146.9
(-1.6)
P 		151
C 296
(3)
P 		144.6
(-1.6)
P 		226.1
(0.9)
P 		96.6
(-3)
P 		199
avr 272.3
(3) 		132.1
(-2.2) 		153.1
(-1.5) 		111.7
(-3) 		161

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 250
1 2 3 4 5 6 range
A 104.1
(-3)
P 		278.2
(3)
P 		117.3
(-2.5)
P 		112.4
(-2.7)
P 		163.5
(-1)
P 		123.1
(-2.3)
P 		174
B 282.1
(2.6)
P 		207
(0.2)
P 		110.9
(-3)
P 		149.2
(-1.7)
P 		201.1
(-0)
P 		293.8
(3)
P 		183
C 338.1
(3)
P 		171.9
(-1)
P 		136.5
(-1.8)
P 		88.3
(-3)
P 		228.5
(0.4)
P 		285.8
(1.7)
P 		250
avr 241.4
(3) 		219
(1.9) 		121.6
(-2.8) 		116.6
(-3) 		197.7
(0.9) 		234.2
(2.7) 		125
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174434_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21381
Sequence Type Exemplar sequence
Representative Public ID CEK003D8R NCBI
Target Description CEK003D8R_rc /REP_DB=TREMBL Accession /GB=D27758 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk3d8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028206(9), NM_001028208(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028206 NCBI Caenorhabditis elegans T12G3.2a (T12G3.2) mRNA, complete cds. 9/11 None
NM_001028208 NCBI Caenorhabditis elegans T12G3.2c (T12G3.2) mRNA, complete cds. 11/11 None
T12G3.2a.2 ENSEMBL cdna:known chromosome:CEL140:IV:12038962:12043425:-1 gene:T12G3.2 11/11 A 
T12G3.2b.1 ENSEMBL cdna:known chromosome:CEL140:IV:12038962:12043568:-1 gene:T12G3.2 11/11 A 
T12G3.2a.1 ENSEMBL cdna:known chromosome:CEL140:IV:12038962:12043425:-1 gene:T12G3.2 9/11 A 
T12G3.2b.2 ENSEMBL cdna:known chromosome:CEL140:IV:12038986:12043479:-1 gene:T12G3.2 11/11 A 
T12G3.2c ENSEMBL cdna:known chromosome:CEL140:IV:12039001:12043425:-1 gene:T12G3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178140 3 2 NM_001028206 174434_at (9), 178615_s_at (11)
NM_001028207 178615_s_at (11)
NM_001028208 174434_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12039001-12039502(-) 100.0 100.0
Public Domain and Genome References
Ensembl T12G3.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92981 Hypothetical protein T12G3.2a [Caenorhabditis elegans] ref|NP_001023377.1| T12G3.2a [Caenorhabditis elegans] 0.0
blast CAE54904 Hypothetical protein T12G3.2b [Caenorhabditis elegans] ref|NP_001023378.1| T12G3.2b [Caenorhabditis elegans] 0.0
blast CAE54905 Hypothetical protein T12G3.2c [Caenorhabditis elegans] ref|NP_001023379.1| T12G3.2c [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:174434_AT
cgctttgattactgattttctgaaactgaaatacacctatcttcatctaattctaattat
ttccattttgcgggtttcttttcatccttcctatcttttttctgcatgttttaattattt
gccttgatttgtattaacgtgtacatttctctaatttctctgcaaatacttgttatctga
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCTTTGATTACTGATTTTCTGAAA 417 253 122 Antisense
GAAATACACCTATCTTCATCTAATT 374 363 149 Antisense
CACCTATCTTCATCTAATTCTAATT 144 201 155 Antisense
TTCTAATTATTTCCATTTTGCGGGT 371 683 172 Antisense
CATTTTGCGGGTTTCTTTTCATCCT 342 215 185 Antisense
TTCCTATCTTTTTTCTGCATGTTTT 63 701 209 Antisense
CTGCATGTTTTAATTATTTGCCTTG 9 239 223 Antisense
ATTTGCCTTGATTTGTATTAACGTG 65 17 238 Antisense
AACGTGTACATTTCTCTAATTTCTC 460 143 257 Antisense
TACATTTCTCTAATTTCTCTGCAAA 67 645 263 Antisense
TCTCTGCAAATACTTGTTATCTGAA 506 611 278 Antisense
Sequence Source Affymetrix Proprietary Database