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[174433_at]

Description C34234_rc /REP_DB=TREMBL Accession /GB=C34234 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk393f12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 68      4grp_highest_8_8 > 36      4grp_high_8_8 > 0      6grp_highest_nonorm > 87      6grp_total > 69      4grp_total > 07      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 865
1 2 3 4 range
A 1404.2
(3)
P 		584.4
(-3)
P 		1304.3
(2.3)
P 		1176.7
(1.3)
P 		820
B 1336.5
(1.8)
P 		898.2
(-3)
P 		987.2
(-2)
P 		1449.5
(3)
P 		551
C 1284.4
(1.6)
P 		885.4
(-3)
P 		1280.2
(1.6)
P 		1404.1
(3)
P 		519
avr 1341.7
(3) 		789.3
(-3) 		1190.6
(1.3) 		1343.4
(3) 		554

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1181
1 2 3 4 5 6 range
A 1176.8
(-0)
P 		1049
(-1)
P 		786.4
(-3)
P 		845.1
(-2.6)
P 		1463.3
(2.1)
P 		1577.4
(3)
P 		791
B 1355.9
(-0.3)
P 		1308.5
(-0.5)
P 		1112
(-1.6)
P 		851.5
(-3)
P 		1335
(-0.4)
P 		1967.7
(3)
P 		1116
C 936.9
(-2.4)
P 		1199.8
(-0.3)
P 		864.8
(-3)
P 		875.1
(-2.9)
P 		927.5
(-2.5)
P 		1597.4
(3)
P 		733
avr 1156.5
(-0.9) 		1185.8
(-0.7) 		921.1
(-2.6) 		857.2
(-3) 		1241.9
(-0.3) 		1714.2
(3) 		857
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 384
1 2 3 4 range
A 511.3
(3)
P 		126.9
(-3)
P 		341
(0.3)
P 		172.2
(-2.3)
P 		384
B 426.2
(3)
P 		221.8
(-3)
P 		228.8
(-2.8)
P 		308.9
(-0.4)
P 		204
C 425.2
(2.4)
P 		254.6
(-2.5)
P 		446.2
(3)
P 		236.8
(-3)
P 		209
avr 454.2
(3) 		201.1
(-3) 		338.7
(0.3) 		239.3
(-2.1) 		253

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 563
1 2 3 4 5 6 range
A 216.9
(-2)
P 		373.1
(1.5)
P 		171.3
(-3)
P 		214.3
(-2)
P 		441.5
(3)
P 		384.1
(1.7)
P 		270
B 471.8
(-0.1)
P 		435.5
(-0.5)
P 		270
(-2.5)
P 		225.5
(-3)
P 		542.4
(0.7)
P 		733.8
(3)
P 		508
C 347.3
(-0.1)
P 		366.1
(0.2)
P 		220.1
(-2.3)
P 		176
(-3)
P 		300.1
(-0.9)
P 		534.8
(3)
P 		359
avr 345.3
(-0.6) 		391.6
(0.2) 		220.5
(-2.7) 		205.3
(-3) 		428
(0.9) 		550.9
(3) 		346
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174433_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20293
Sequence Type Exemplar sequence
Representative Public ID C34234 NCBI
Target Description C34234_rc /REP_DB=TREMBL Accession /GB=C34234 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk393f12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059984(11), NM_182068(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059984 NCBI Caenorhabditis elegans F36A2.9a (phospholipase) mRNA, complete cds. 11/11 None
NM_182068 NCBI Caenorhabditis elegans F36A2.9b (phospholipase) mRNA, complete cds. 11/11 None
F36A2.9b ENSEMBL cdna:known chromosome:CEL140:I:8825106:8829463:1 gene:F36A2.9 11/11 A 
F36A2.9a ENSEMBL cdna:known chromosome:CEL140:I:8825106:8829464:1 gene:F36A2.9 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8829056-8829337(+) 100.0 100.0
Public Domain and Genome References
Ensembl F36A2.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03072 Hypothetical protein F36A2.9a [Caenorhabditis elegans] ref|NP_492385.2| F36A2.9a [Caenorhabditis elegans] 0.0
blast CAD56582 Hypothetical protein F36A2.9b [Caenorhabditis elegans] ref|NP_871868.1| F36A2.9b [Caenorhabditis elegans] 0.0
blast CAE66981 Hypothetical protein CBG12377 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001087 EMBL-EBI Lipolytic enzyme, G-D-S-L 3.1E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_871868.1 1 468-490
F36A2.9b 1 468-490
Sequence
Target Sequence 
>C. ELEGANS:174433_AT
gtaattgctgtattttcatcttgtcatcactcactctctgtaatttttatccaattatcc
tgtaaatttatcatttacatccttgaattcattccaacccagttttgctttacaacctca
gttttatggcatttgtaaataatttatgccaatcaattccgaaaatctaagtcactttca
acctccagtcaacagagttccctacgtactttacgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAATTGCTGTATTTTCATCTTGTC 437 463 29 Antisense
GTATTTTCATCTTGTCATCACTCAC 461 453 38 Antisense
TCATCACTCACTCTCTGTAATTTTT 225 621 52 Antisense
CTCACTCTCTGTAATTTTTATCCAA 61 229 58 Antisense
TGTAATTTTTATCCAATTATCCTGT 561 559 67 Antisense
AAATTTATCATTTACATCCTTGAAT 101 117 92 Antisense
ACATCCTTGAATTCATTCCAACCCA 182 105 105 Antisense
CAATTCCGAAAATCTAAGTCACTTT 474 167 182 Antisense
AATCTAAGTCACTTTCAACCTCCAG 465 151 192 Antisense
TCACTTTCAACCTCCAGTCAACAGA 219 623 200 Antisense
CAGAGTTCCCTACGTACTTTACGAT 77 203 221 Antisense
Sequence Source Affymetrix Proprietary Database