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[174427_s_at] isocitrate dehydrogenase

Description C29603_rc /REP_DB=TREMBL Accession /GB=C29603 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk216a10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 38      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 57      6grp_total > 37      4grp_total > 31      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1284
1 2 3 4 range
A 3801.2
(3)
P 		2517
(-3)
P 		2930
(-1.1)
P 		3165.9
(0)
P 		1284
B 3405.4
(3)
P 		2573.6
(-3)
P 		2803.9
(-1.3)
P 		3283.9
(2.1)
P 		832
C 3509.2
(3)
P 		2910.5
(-2.5)
P 		3082.2
(-0.9)
P 		2857.2
(-3)
P 		652
avr 3571.9
(3) 		2667
(-3) 		2938.7
(-1.2) 		3102.3
(-0.1) 		905

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1417
1 2 3 4 5 6 range
A 3273.1
(3)
P 		2679.4
(-0.4)
P 		2773.5
(0.1)
P 		2237.6
(-3)
P 		2576.6
(-1)
P 		2548.6
(-1.2)
P 		1036
B 3573.5
(3)
P 		2940
(0.3)
P 		2156.3
(-3)
P 		2437.4
(-1.8)
P 		2642.2
(-0.9)
P 		3550
(2.9)
P 		1417
C 3130.5
(3)
P 		2894.9
(1.5)
P 		2707.4
(0.4)
P 		2495.5
(-0.9)
P 		2158.9
(-3)
P 		2956.5
(1.9)
P 		972
avr 3325.7
(3) 		2838.1
(-0.1) 		2545.7
(-2) 		2390.2
(-3) 		2459.2
(-2.6) 		3018.4
(1) 		936
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 921
1 2 3 4 range
A 1384
(3)
P 		546.4
(-2.5)
P 		766
(-1)
P 		463.4
(-3)
P 		921
B 1086
(3)
P 		635.6
(-3)
P 		649.7
(-2.8)
P 		699.8
(-2.1)
P 		450
C 1161.7
(3)
P 		836.8
(0.1)
P 		1074.2
(2.2)
P 		481.8
(-3)
P 		680
avr 1210.6
(3) 		672.9
(-1.9) 		830
(-0.4) 		548.3
(-3) 		662

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 822
1 2 3 4 5 6 range
A 603.4
(-2.4)
P 		952.9
(3)
P 		604
(-2.4)
P 		567.5
(-3)
P 		777.5
(0.3)
P 		620.5
(-2.2)
P 		385
B 1243.4
(2.4)
P 		978.6
(0.4)
P 		523.6
(-3)
P 		645.6
(-2.1)
P 		1073.5
(1.1)
P 		1323.8
(3)
P 		800
C 1160.3
(3)
P 		883.3
(0.5)
P 		689.1
(-1.3)
P 		501.9
(-3)
P 		698.6
(-1.2)
P 		989.7
(1.4)
P 		658
avr 1002.4
(3) 		938.3
(2.1) 		605.6
(-2.5) 		571.7
(-3) 		849.9
(0.9) 		978
(2.7) 		431
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174427_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20879
Sequence Type Exemplar sequence
Representative Public ID C29603 NCBI
Target Description C29603_rc /REP_DB=TREMBL Accession /GB=C29603 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk216a10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065526(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065526 NCBI Caenorhabditis elegans F35G12.2 (F35G12.2) mRNA, complete cds. 11/11 None
F35G12.2.2 ENSEMBL cdna:known chromosome:CEL140:III:4567290:4571817:1 gene:F35G12.2 11/11 A 
F35G12.2.3 ENSEMBL cdna:known chromosome:CEL140:III:4568762:4571817:1 gene:F35G12.2 11/11 A 
F35G12.2.4 ENSEMBL cdna:known chromosome:CEL140:III:4568976:4571377:1 gene:F35G12.2 11/11 A 
F35G12.2.1 ENSEMBL cdna:known chromosome:CEL140:III:4568978:4571817:1 gene:F35G12.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4571034-4571325(+) 100.0 100.0
Public Domain and Genome References
Gene Title isocitrate dehydrogenase
Ensembl F35G12.2
Entrez Gene 175598 Entrez gene
SwissProt Q20049 EMBL-EBI
WormBase CE31500 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15139_S_AT isocitrate dehydrogenase subunit 2 / NAD+ isocitrate dehydrogenase subunit 2 at
ATH1-121501:263583_AT isocitrate dehydrogenase subunit 2 / NAD+ isocitrate dehydrogenase subunit 2 at
CANINE:1583519_S_AT similar to Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial precursor (Isocitric dehydrogenase) (NAD(+)-specific ICDH) cfa
CANINE:1583520_X_AT similar to Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial precursor (Isocitric dehydrogenase) (NAD(+)-specific ICDH) cfa
CANINE:1583518_AT similar to Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial precursor (Isocitric dehydrogenase) (NAD(+)-specific ICDH) cfa
CANINE_2:CFAAFFX.29440.1.S1_S_AT similar to Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial precursor (Isocitric dehydrogenase) (NAD(+)-specific ICDH) cfa
DROSOPHILA_2:1629691_AT dm
DROSGENOME1:150603_AT dm
HU35KSUBA:Z68907_S_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HU35KSUBD:RC_H18412_S_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HUGENEFL:Z68129_CDS1_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HG-U133A:202471_S_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HG-U133_PLUS_2:202471_S_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HG-FOCUS:202471_S_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HG-U133A_2:202471_S_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HG-U133_PLUS_2:214333_X_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HG-U133A_2:214333_X_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HG-U133A:214333_X_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
U133_X3P:G4758581_3P_A_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
U133_X3P:HS.75253.1.A1_3P_X_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
U133_X3P:HS.75253.1.A1_3P_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
U133_X3P:HS.75253.1.A1_3P_A_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
U133_X3P:214333_3P_X_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
HG-U95AV2:36574_AT isocitrate dehydrogenase 3 (NAD+) gamma hs
MU11KSUBA:U68564_S_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MG-U74AV2:93029_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MG-U74AV2:162478_R_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MG-U74CV2:170360_R_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MOE430A:1416789_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MOUSE430A_2:1416789_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MOUSE430_2:1416789_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MOE430A:1416788_A_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MOUSE430A_2:1416788_A_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MOUSE430_2:1416788_A_AT isocitrate dehydrogenase 3 (NAD+), gamma mm
MU19KSUBA:TC22688_AT Isocitrate dehydrogenase 3 (NAD+), gamma (Idh3g), mRNA mm
RG-U34A:RC_AA892808_AT isocitrate dehydrogenase 3, gamma rn
RAE230A:1370865_AT isocitrate dehydrogenase 3, gamma rn
RAT230_2:1370865_AT isocitrate dehydrogenase 3, gamma rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6099 tricarboxylic acid cycle inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5739 mitochondrion inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4449 isocitrate dehydrogenase (NAD+) activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86325 Hypothetical protein F35G12.2 [Caenorhabditis elegans] ref|NP_497927.2| F35G12.2 [Caenorhabditis elegans] 0.0
blast CAE71183 Hypothetical protein CBG18040 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001804 EMBL-EBI Isocitrate/isopropylmalate dehydrogenase 2.0E-85
Sequence
Target Sequence 
>C. ELEGANS:174427_S_AT
taccgtgtctaatttctacctgataatcgaaagtttgaaacatcccacatagtggctagc
tttacccttacgttcttttcctctgtttttttttcgattaatccatgattcgtagcataa
tgcgttactcattgtgctctttctctgtttgaccccgttgactgaacgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACCGTGTCTAATTTCTACCTGATA 273 649 67 Antisense
GTGTCTAATTTCTACCTGATAATCG 374 487 71 Antisense
AATTTCTACCTGATAATCGAAAGTT 210 175 77 Antisense
AAAGTTTGAAACATCCCACATAGTG 295 121 96 Antisense
GAAACATCCCACATAGTGGCTAGCT 562 355 103 Antisense
CCACATAGTGGCTAGCTTTACCCTT 143 275 111 Antisense
TCCATGATTCGTAGCATAATGCGTT 151 591 168 Antisense
GCATAATGCGTTACTCATTGTGCTC 434 309 181 Antisense
AATGCGTTACTCATTGTGCTCTTTC 291 167 185 Antisense
TCTGTTTGACCCCGTTGACTGAACG 313 599 210 Antisense
TGTTTGACCCCGTTGACTGAACGTT 162 555 212 Antisense
Sequence Source Affymetrix Proprietary Database