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[174424_at]

Description CEK005B4R_rc /REP_DB=TREMBL Accession /GB=D27858 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk5b4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 16      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 30      6grp_total > 25      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 846
1 2 3 4 range
A 1168.1
(3)
P 		357.6
(-3)
P 		412.2
(-2.6)
P 		382.1
(-2.8)
P 		811
B 728.1
(3)
P 		603.4
(1.2)
P 		322.6
(-3)
P 		465.3
(-0.9)
P 		406
C 908.2
(3)
P 		558.6
(-1)
P 		460.8
(-2.2)
P 		387.4
(-3)
P 		521
avr 934.8
(3) 		506.5
(-1.8) 		398.5
(-3) 		411.6
(-2.9) 		536

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 876
1 2 3 4 5 6 range
A 943.2
(3)
P 		794.9
(1.1)
P 		518.7
(-2.4)
P 		470.4
(-3)
P 		576.5
(-1.7)
P 		588.2
(-1.5)
P 		473
B 1345.9
(3)
P 		1134.9
(1.5)
P 		805.8
(-0.7)
P 		698.6
(-1.4)
P 		473.1
(-3)
P 		640.1
(-1.9)
P 		873
C 843
(2.1)
P 		900.4
(3)
P 		784.3
(1.3)
P 		807.6
(1.6)
P 		499.3
(-3)
P 		584.9
(-1.7)
P 		401
avr 1044
(3) 		943.4
(1.9) 		702.9
(-0.9) 		658.9
(-1.4) 		516.3
(-3) 		604.4
(-2) 		528
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 369
1 2 3 4 range
A 425.3
(3)
P 		77.6
(-2.6)
P 		107.8
(-2.2)
P 		55.9
(-3)
P 		369
B 232.2
(3)
P 		149
(-0.2)
P 		74.8
(-3)
P 		99.2
(-2.1)
P 		157
C 300.6
(3)
P 		160.6
(-0.6)
P 		160.6
(-0.6)
P 		65.3
(-3)
P 		235
avr 319.4
(3) 		129.1
(-1.6) 		114.4
(-2) 		73.5
(-3) 		246

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 355
1 2 3 4 5 6 range
A 173.9
(-0.8)
P 		282.7
(3)
P 		113
(-3)
P 		119.3
(-2.8)
P 		174
(-0.8)
P 		143.2
(-1.9)
P 		170
B 468.3
(3)
P 		377.8
(1.1)
P 		195.7
(-2.8)
P 		185
(-3)
P 		192.2
(-2.8)
P 		238.7
(-1.9)
P 		283
C 312.5
(3)
P 		274.7
(1.5)
P 		199.6
(-1.5)
P 		162.4
(-3)
P 		161.6
(-3)
P 		195.8
(-1.6)
P 		151
avr 318.2
(3) 		311.7
(2.8) 		169.4
(-2.5) 		155.6
(-3) 		175.9
(-2.3) 		192.6
(-1.6) 		163
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174424_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21034
Sequence Type Exemplar sequence
Representative Public ID CEK005B4R NCBI
Target Description CEK005B4R_rc /REP_DB=TREMBL Accession /GB=D27858 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk5b4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027552(11), NM_066086(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027552 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-152) (tag-152) mRNA, complete cds. 11/11 None
NM_066086 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-152) (tag-152) mRNA, complete cds. 11/11 None
R13F6.4a ENSEMBL cdna:known chromosome:CEL140:III:6838239:6847217:1 gene:R13F6.4 11/11 A 
R13F6.4b ENSEMBL cdna:known chromosome:CEL140:III:6838239:6847188:1 gene:R13F6.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175953 3 2 NM_001027552 174424_at (11), 183650_at (11)
NM_066086 174424_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6846823-6847191(+) 100.0 100.0
Public Domain and Genome References
Ensembl R13F6.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022723 Temporarily Assigned Gene name family member (tag-152) [Caenorhabditis elegans] 0.0
blast BAD91086.1 Ten-1L [Caenorhabditis elegans] 0.0
blast NP_741203 Temporarily Assigned Gene name family member (tag-152) [Caenorhabditis elegans] gb|AAC47044.3| Temporarily assigned gene name protein 152, isoform a [Caenorhabditis elegans] 0.0
blast NP_498487 Temporarily Assigned Gene name family member (tag-152) [Caenorhabditis elegans] gb|AAN65304.1| Temporarily assigned gene name protein 152, isoform c [Caenorhabditis elegans] 0.0
blast BAD91087.1 Ten-1S [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 3.8E-5
Pfam IPR001258 EMBL-EBI NHL repeat 3.2
Pfam IPR001258 EMBL-EBI NHL repeat 0.033
Pfam IPR001258 EMBL-EBI NHL repeat 2.8
Pfam IPR001258 EMBL-EBI NHL repeat 0.023
Pfam IPR001258 EMBL-EBI NHL repeat 9.8E-7
Pfam IPR006209 EMBL-EBI EGF-like domain 3.8E-5
Pfam IPR001258 EMBL-EBI NHL repeat 3.2
Pfam IPR001258 EMBL-EBI NHL repeat 0.033
Pfam IPR001258 EMBL-EBI NHL repeat 2.8
Pfam IPR001258 EMBL-EBI NHL repeat 0.023
Pfam IPR001258 EMBL-EBI NHL repeat 9.8E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022723.1 1 259-281
R13F6.4a 1 93-115
R13F6.4b 1 93-115
Sequence
Target Sequence 
>C. ELEGANS:174424_AT
tgttatccgtaatttcctgaaactcctagtgaaaaatgcatctcacttcgggtcctccca
attgcatttttctgaactcctctgaccttaactttttgacatctgaaactcacgggaagt
ccctttttctgcagctcatcatcacttttttttaaagaaaaacttttaattagagcttta
ataatgtcctttcaaatgaaacccctccgccaaatcaatgacgtgtaattacaatatccc
ctcatttcattttctccctttccaacccctcaagagatttcgatatttatttatatctgt
agaacttattgttgttgaatgcgcttttatgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTATCCGTAATTTCCTGAAACTC 376 561 15 Antisense
GAAAAATGCATCTCACTTCGGGTCC 595 353 45 Antisense
CTCCTCTGACCTTAACTTTTTGACA 402 235 91 Antisense
TTTTGACATCTGAAACTCACGGGAA 582 677 108 Antisense
CTCACGGGAAGTCCCTTTTTCTGCA 646 225 123 Antisense
TGCAGCTCATCATCACTTTTTTTTA 6 579 144 Antisense
TGTCCTTTCAAATGAAACCCCTCCG 278 553 199 Antisense
CCCTCCGCCAAATCAATGACGTGTA 85 257 217 Antisense
GTAATTACAATATCCCCTCATTTCA 347 461 239 Antisense
CTTTCCAACCCCTCAAGAGATTTCG 586 223 272 Antisense
TTGTTGTTGAATGCGCTTTTATGAA 258 707 323 Antisense
Sequence Source Affymetrix Proprietary Database