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[174415_at]

Description C38097_rc /REP_DB=TREMBL Accession /GB=C38097 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk486g8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 07      4grp_highest_8_8 > 32      4grp_high_8_8 > 43      6grp_highest_nonorm > 40      6grp_total > 08      4grp_total > 52      6grp_highest_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 447
1 2 3 4 range
A 590.6
(2.1)
P 		646.3
(3)
P 		461.4
(0.1)
P 		262.4
(-3)
P 		384
B 517.6
(1)
P 		669.6
(3)
P 		361.8
(-1.1)
P 		223.1
(-3)
P 		447
C 560.5
(1.8)
P 		642.6
(3)
P 		502.5
(0.9)
P 		239.9
(-3)
P 		403
avr 556.2
(1.6) 		652.8
(3) 		441.9
(-0.1) 		241.8
(-3) 		411

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1015
1 2 3 4 5 6 range
A 930.3
(3)
P 		586.8
(-1.4)
P 		619.5
(-1)
P 		547.9
(-1.9)
P 		463.1
(-3)
P 		495.8
(-2.6)
P 		467
B 1264.4
(3)
P 		618.8
(-0.8)
P 		414.7
(-2)
P 		747.5
(-0.1)
P 		249.2
(-3)
P 		571.5
(-1.1)
P 		1015
C 1013.5
(3)
P 		648.7
(-0.7)
P 		593.6
(-1.3)
P 		543.2
(-1.8)
P 		420.7
(-3)
P 		520.7
(-2)
P 		593
avr 1069.4
(3) 		618.1
(-0.9) 		542.6
(-1.6) 		612.9
(-1) 		377.7
(-3) 		529.3
(-1.7) 		692
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 215
(3)
P 		140.3
(0.5)
P 		120.6
(-0.2)
P 		38.4
(-3)
P 		177
B 165.1
(3)
P 		165.4
(3)
P 		83.8
(-1.2)
P 		47.5
(-3)
P 		118
C 185.5
(3)
P 		184.8
(3)
P 		175.1
(2.6)
P 		40.4
(-3)
P 		145
avr 188.5
(3) 		163.5
(2) 		126.5
(0.5) 		42.1
(-3) 		146

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 339
1 2 3 4 5 6 range
A 171.5
(0.5)
P 		208.7
(3)
P 		134.9
(-2)
P 		139
(-1.8)
P 		139.8
(-1.7)
P 		120.7
(-3)
P 		88
B 439.9
(3)
P 		206
(-1.1)
P 		100.7
(-3)
P 		198
(-1.3)
P 		101.3
(-3)
P 		213.1
(-1)
P 		339
C 375.7
(3)
P 		197.9
(-1)
P 		151.1
(-2.1)
P 		109.2
(-3)
P 		136.1
(-2.4)
P 		174.3
(-1.5)
P 		267
avr 329
(3) 		204.2
(-0.7) 		128.9
(-2.9) 		148.7
(-2.3) 		125.7
(-3) 		169.4
(-1.7) 		203
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174415_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22410
Sequence Type Exemplar sequence
Representative Public ID C38097 NCBI
Target Description C38097_rc /REP_DB=TREMBL Accession /GB=C38097 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk486g8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076098(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076098 NCBI Caenorhabditis elegans F53B3.5 (F53B3.5) mRNA, complete cds. 11/11 None
F53B3.5 ENSEMBL cdna:known chromosome:CEL140:X:2872801:2879612:-1 gene:F53B3.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2872810-2873115(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53B3.5
Entrez Gene 180578 Entrez gene
SwissProt Q20713 EMBL-EBI
WormBase CE30142 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1634191_AT dm
DROSGENOME1:154753_AT dm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5923 tight junction inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81408 Hypothetical protein F53B3.5 [Caenorhabditis elegans] ref|NP_508499.2| F53B3.5 [Caenorhabditis elegans] 1.0E-179
blast CAE58820 Hypothetical protein CBG02030 [Caenorhabditis briggsae] 1.0E-137
blast T16454 hypothetical protein F53B3.5 - Caenorhabditis elegans 2.0E-81
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508499.2 5 20-42,142-164,174-196,228-250,297-319
F53B3.5 5 20-42,142-164,174-196,228-250,297-319
Sequence
Target Sequence 
>C. ELEGANS:174415_AT
ttttttctcaactttgtcattctgagtttagtgttttcttgttctgattttgttggcaat
ccccaatccgttttatgtccgtcttctcatttttgtactgccactccttgtttgaaatta
tcgagtttggatggaagacccttggtgctccttttcccacttgtcttattcaaaatgcca
tctgccccctttttttgttcagttcgttcagcaagcacaatgaatttaaaagataatcga
tactcgaatatgccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTTTCTCAACTTTGTCATTCTGA 395 675 31 Antisense
GTCATTCTGAGTTTAGTGTTTTCTT 693 467 46 Antisense
TAGTGTTTTCTTGTTCTGATTTTGT 615 653 59 Antisense
GTTCTGATTTTGTTGGCAATCCCCA 83 443 71 Antisense
ATTTTGTTGGCAATCCCCAATCCGT 451 13 77 Antisense
TTATGTCCGTCTTCTCATTTTTGTA 350 681 103 Antisense
TACTGCCACTCCTTGTTTGAAATTA 57 647 126 Antisense
TGGATGGAAGACCCTTGGTGCTCCT 629 549 158 Antisense
CTTTTTTTGTTCAGTTCGTTCAGCA 199 59 219 Antisense
GTTCAGTTCGTTCAGCAAGCACAAT 437 443 227 Antisense
GATAATCGATACTCGAATATGCCAC 92 423 262 Antisense
Sequence Source Affymetrix Proprietary Database