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[174407_at]

Description C32229_rc /REP_DB=TREMBL Accession /GB=C32229 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk326d7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 68      6grp_total > 95      4grp_total > 44      6grp_cgc > 88      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 487
1 2 3 4 range
A 1169.2
(1.3)
P 		1307.9
(3)
P 		1062.3
(-0)
P 		822.5
(-3)
P 		485
B 1009.6
(0.9)
P 		1109.8
(3)
P 		820.5
(-3)
P 		966.5
(0)
P 		289
C 1169.9
(3)
P 		1097.7
(0.4)
P 		1038.6
(-1.7)
P 		1003.1
(-3)
P 		167
avr 1116.2
(1.6) 		1171.8
(3) 		973.8
(-1.9) 		930.7
(-3) 		241

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2064
1 2 3 4 5 6 range
A 1884
(-0.5)
P 		1175.1
(-3)
P 		1484.2
(-1.9)
P 		2348.3
(1.1)
P 		2880.9
(3)
P 		2342.1
(1.1)
P 		1706
B 1966.8
(0.9)
P 		1496.6
(-0.7)
P 		817
(-3)
P 		2195.8
(1.7)
P 		2592.8
(3)
P 		2284.5
(2)
P 		1776
C 1550.1
(-1.9)
P 		1370.9
(-2.6)
P 		1267.3
(-3)
P 		2247.6
(0.8)
P 		2818.8
(3)
P 		2046.3
(0)
P 		1552
avr 1800.3
(-0.7) 		1347.5
(-2.4) 		1189.5
(-3) 		2263.9
(1.1) 		2764.2
(3) 		2224.3
(0.9) 		1575
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 305
1 2 3 4 range
A 425.7
(3)
P 		283.9
(0.2)
P 		277.7
(0.1)
P 		120.4
(-3)
P 		305
B 321.9
(3)
P 		274.1
(0.8)
P 		190.1
(-3)
P 		206
(-2.3)
P 		132
C 387.3
(3)
P 		315.6
(1)
P 		362
(2.3)
P 		169.1
(-3)
P 		218
avr 378.3
(3) 		291.2
(0.5) 		276.6
(0.1) 		165.2
(-3) 		213

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 855
1 2 3 4 5 6 range
A 347.3
(-2.7)
P 		417.9
(-2)
P 		323.2
(-3)
P 		595.6
(-0)
P 		869.3
(3)
P 		570.2
(-0.3)
P 		546
B 684.4
(0.4)
P 		498.1
(-0.9)
P 		198.4
(-3)
P 		581.6
(-0.3)
P 		1053.4
(3)
P 		851.9
(1.6)
P 		855
C 574.5
(-0.4)
P 		418.3
(-2)
P 		322.6
(-3)
P 		452
(-1.7)
P 		912.1
(3)
P 		685
(0.7)
P 		590
avr 535.4
(-0.7) 		444.8
(-1.5) 		281.4
(-3) 		543.1
(-0.6) 		944.9
(3) 		702.4
(0.8) 		664
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174407_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22076
Sequence Type Exemplar sequence
Representative Public ID C32229 NCBI
Target Description C32229_rc /REP_DB=TREMBL Accession /GB=C32229 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk326d7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077029(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077029 NCBI Caenorhabditis elegans Tetra THymosin (four thymosin repeat protein) family member (tth-1) (tth-1) mRNA, complete cds. 11/11 None
F08F1.8.3 ENSEMBL cdna:known chromosome:CEL140:X:8422416:8423670:-1 gene:F08F1.8 11/11 A 
F08F1.8.1 ENSEMBL cdna:known chromosome:CEL140:X:8422416:8423928:-1 gene:F08F1.8 11/11 A 
F08F1.8.2 ENSEMBL cdna:known chromosome:CEL140:X:8422416:8423668:-1 gene:F08F1.8 11/11 A 
F08F1.8.4 ENSEMBL cdna:known chromosome:CEL140:X:8422441:8423928:-1 gene:F08F1.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8422484-8422907(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tth-1
Ensembl F08F1.8
Entrez Gene 181097 Entrez gene
SwissProt O17389 EMBL-EBI
WormBase CE27929 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7010 cytoskeleton organization and biogenesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3779 actin binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB71308 Tetra thymosin (four thymosin repeat protein) protein 1 [Caenorhabditis elegans] ref|NP_509430.1| Tetra THymosin (four thymosin repeat protein) family member (tth-1) [Caenorhabditis elegans] 1.0E-79
blast CAE74899 Hypothetical protein CBG22765 [Caenorhabditis briggsae] 6.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR001152 EMBL-EBI Thymosin beta-4 3.1E-6
Pfam IPR001152 EMBL-EBI Thymosin beta-4 1.1E-6
Sequence
Target Sequence 
>C. ELEGANS:174407_AT
tgaaaaagagtccctccttcttcaacctcaattcggtacgccttcatattatcgttagtg
aaaaatccggactctcacatttaaatgcgttttctttgttttctggttcgagttttatcg
attttaacattttaccctgacaaacatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAAAAGAGTCCCTCCTTCTTCAA 240 573 123 Antisense
CTCCTTCTTCAACCTCAATTCGGTA 279 233 136 Antisense
CTCAATTCGGTACGCCTTCATATTA 514 227 149 Antisense
GGTACGCCTTCATATTATCGTTAGT 574 503 157 Antisense
GAAAAATCCGGACTCTCACATTTAA 461 355 182 Antisense
TCCGGACTCTCACATTTAAATGCGT 247 593 188 Antisense
TTAAATGCGTTTTCTTTGTTTTCTG 678 681 203 Antisense
TGCGTTTTCTTTGTTTTCTGGTTCG 235 583 208 Antisense
TTGTTTTCTGGTTCGAGTTTTATCG 579 707 218 Antisense
TATCGATTTTAACATTTTACCCTGA 190 655 238 Antisense
TTAACATTTTACCCTGACAAACATG 259 683 246 Antisense
Sequence Source Affymetrix Proprietary Database