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[174405_at]

Description C54078_rc /REP_DB=TREMBL Accession /GB=C54078 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk357g8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 69      4grp_highest_8_8 > 36      4grp_high_8_8 > 0      6grp_highest_nonorm > 91      6grp_total > 79      4grp_total > 07      6grp_highest_sub > Cu      6grp_cgc > 19      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 219.6
(3)
P 		162.9
(-3)
P 		163.6
(-2.9)
P 		166.8
(-2.6)
P 		57
B 204.7
(3)
P 		104.9
(-3)
P 		159.4
(0.3)
P 		174.4
(1.2)
P 		100
C 225.4
(0.5)
P 		138.4
(-3)
P 		194
(-0.8)
P 		288.1
(3)
P 		150
avr 216.6
(3) 		135.4
(-3) 		172.3
(-0.3) 		209.8
(2.5) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 206.5
(1.6)
P 		196.4
(0.7)
P 		222.4
(3)
P 		189.6
(0.1)
P 		206.3
(1.6)
P 		155
(-3)
P 		67
B 194.1
(-0.7)
P 		222.5
(0.6)
P 		144.6
(-3)
P 		177.6
(-1.5)
P 		255
(2.1)
P 		275.3
(3)
P 		131
C 171.3
(-1.6)
P 		192.7
(-0.4)
P 		147.5
(-3)
P 		206.5
(0.4)
P 		178.6
(-1.2)
P 		252.1
(3)
P 		105
avr 190.6
(-1) 		203.9
(0.5) 		171.5
(-3) 		191.2
(-0.9) 		213.3
(1.5) 		227.5
(3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 79.9
(3)
P 		35.4
(-1.8)
P 		42.8
(-1)
P 		24.4
(-3)
P 		56
B 65.3
(3)
P 		25.9
(-3)
P 		36.9
(-1.3)
P 		37.2
(-1.3)
P 		39
C 74.6
(3)
P 		39.8
(-3)
P 		67.6
(1.8)
P 		48.6
(-1.5)
P 		35
avr 73.3
(3) 		33.7
(-3) 		49.1
(-0.7) 		36.7
(-2.5) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 38.1
(-2.9)
P 		69.8
(3)
P 		48.4
(-1)
P 		48.1
(-1.1)
P 		62.3
(1.6)
P 		37.7
(-3)
P 		32
B 67.6
(-0.2)
P 		74.1
(0.4)
P 		35.1
(-3)
P 		47
(-2)
P 		103.6
(3)
P 		102.7
(2.9)
P 		69
C 63.5
(0.3)
P 		58.8
(-0.3)
P 		37.5
(-3)
P 		41.5
(-2.5)
P 		57.8
(-0.4)
P 		84.4
(3)
P 		47
avr 56.4
(-0.2) 		67.6
(1.7) 		40.3
(-3) 		45.5
(-2.1) 		74.6
(2.9) 		74.9
(3) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174405_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21762
Sequence Type Exemplar sequence
Representative Public ID C54078 NCBI
Target Description C54078_rc /REP_DB=TREMBL Accession /GB=C54078 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk357g8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072085(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072085 NCBI Caenorhabditis elegans Adenylyl CYclase family member (acy-4) (acy-4) mRNA, complete cds. 11/11 None
SNAP00000017873 ENSEMBL cdna:SNAP chromosome:CEL140:V:5627531:5633473:-1 9/11 None
T01C2.1 ENSEMBL cdna:known chromosome:CEL140:V:5627330:5633473:-1 gene:T01C2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5627397-5627677(-) 99.64 99.64
Public Domain and Genome References
Gene Title
Gene Symbol acy-4
Ensembl T01C2.1
Entrez Gene 178949 Entrez gene
SwissProt Q966F9 EMBL-EBI
WormBase CE37665 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1582776_AT adenylate cyclase 6 cfa
CANINE_2:CFAAFFX.14058.1.S1_S_AT adenylate cyclase 6 cfa
DROSOPHILA_2:1633015_AT dm
DROSGENOME1:148898_AT dm
DROSOPHILA_2:1641693_S_AT dm
DROSGENOME1:141806_AT dm
HU35KSUBB:RC_F10815_F_AT adenylate cyclase 6 hs
HU35KSUBD:RC_R72886_S_AT adenylate cyclase 6 hs
HG-U133A:209195_S_AT adenylate cyclase 6 hs
HG-U133_PLUS_2:209195_S_AT adenylate cyclase 6 hs
HG-FOCUS:209195_S_AT adenylate cyclase 6 hs
HG-U133A_2:209195_S_AT adenylate cyclase 6 hs
U133_X3P:G9049782_3P_A_AT adenylate cyclase 6 hs
HG-U95AV2:39383_AT adenylate cyclase 6 hs
HG-U133A_2:216743_AT Adenylate cyclase 6 hs
HG-U133_PLUS_2:216743_AT Adenylate cyclase 6 hs
HG-U133A:216743_AT Adenylate cyclase 6 hs
MU11KSUBA:M93422_S_AT adenylate cyclase 6 mm
MG-U74AV2:102321_AT adenylate cyclase 6 mm
MOE430A:1418128_AT adenylate cyclase 6 mm
MOUSE430A_2:1418128_AT adenylate cyclase 6 mm
MOUSE430_2:1418128_AT adenylate cyclase 6 mm
MU19KSUBC:TC36198_AT Adenylate cyclase 6 (Adcy6), mRNA mm
MU19KSUBC:TC36198_G_AT Adenylate cyclase 6 (Adcy6), mRNA mm
RAE230A:1386876_AT adenylate cyclase 6 rn
RAT230_2:1386876_AT adenylate cyclase 6 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5886 plasma membrane inferred from curator QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4016 adenylate cyclase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK71395 Adenylyl cyclase protein 4 [Caenorhabditis elegans] ref|NP_504486.3| Adenylyl CYclase family member (acy-4) [Caenorhabditis elegans] 0.0
blast CAE64598 Hypothetical protein CBG09353 [Caenorhabditis briggsae] 0.0
blast EAA07942 ENSANGP00000017563 [Anopheles gambiae str. PEST] ref|XP_311887.2| ENSANGP00000017563 [Anopheles gambiae str. PEST] 1.0E-163
blast BAE23593.1 unnamed protein product [Mus musculus] 1.0E-137
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 4.9E-63
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.6E-72
Pfam IPR001054 EMBL-EBI Guanylate cyclase 4.9E-63
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.6E-72
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504486.3 7 112-134,149-171,522-544,549-571,578-600,642-661,668-690
T01C2.1 7 112-134,149-171,522-544,549-571,578-600,642-661,668-690
SNAP00000017873 6 89-111,121-143,491-513,518-537,550-572,582-604
Sequence
Target Sequence 
>C. ELEGANS:174405_AT
atcaatactggaaccactcgggtacaatttcgaatgtcgcggtcaaatcaatgtaaaagg
aaaaggcatgatggaaacattcttcttattaccacctgaggatttcgattgtcaacttta
tcattgatacatttttgtgttattttgtgttattcatatgggtagaatatactttataga
aaccaatcatttttcttgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAATACTGGAACCACTCGGGTAC 469 25 33 Antisense
GAACCACTCGGGTACAATTTCGAAT 74 345 43 Antisense
GGGTACAATTTCGAATGTCGCGGTC 117 495 52 Antisense
CGAATGTCGCGGTCAAATCAATGTA 255 253 63 Antisense
AAGGCATGATGGAAACATTCTTCTT 435 161 95 Antisense
GAAACATTCTTCTTATTACCACCTG 209 357 106 Antisense
CATTCTTCTTATTACCACCTGAGGA 314 215 110 Antisense
TACCACCTGAGGATTTCGATTGTCA 626 649 122 Antisense
GGATTTCGATTGTCAACTTTATCAT 513 509 132 Antisense
CATTTTTGTGTTATTTTGTGTTATT 330 215 162 Antisense
TAGAAACCAATCATTTTTCTTGAAA 350 653 209 Antisense
Sequence Source Affymetrix Proprietary Database