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[174404_s_at]

Description C57239_rc /REP_DB=TREMBL Accession /GB=C57239 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk300h2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 76      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 69      6grp_total > 66      4grp_total > 09      6grp_highest_sub > Da      6grp_high1_sub > Da      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5859
1 2 3 4 range
A 1044.9
(-3)
P 		2109.8
(-1.8)
P 		1811.6
(-2.2)
P 		6519.2
(3)
P 		5474
B 1057.3
(-3)
P 		1872.8
(-2.2)
P 		1821.1
(-2.2)
P 		6904.1
(3)
P 		5847
C 1095
(-3)
P 		1792.6
(-2.2)
P 		1953.2
(-2)
P 		6381.6
(3)
P 		5287
avr 1065.7
(-3) 		1925.1
(-2.1) 		1862
(-2.1) 		6601.6
(3) 		5536

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4645
1 2 3 4 5 6 range
A 1005.7
(-3)
P 		1520.6
(-2.3)
P 		2488.6
(-1)
P 		2096.6
(-1.5)
P 		994.1
(-3)
P 		5403.8
(3)
P 		4410
B 1307.3
(-3)
P 		1820.8
(-2.2)
P 		2383
(-1.4)
P 		1584.9
(-2.6)
P 		1337
(-3)
P 		5222.4
(3)
P 		3915
C 1375.4
(-2.7)
P 		1582.7
(-2.4)
P 		1926.6
(-1.9)
P 		2303.8
(-1.4)
P 		1126.2
(-3)
P 		5638.9
(3)
P 		4513
avr 1229.5
(-2.9) 		1641.4
(-2.3) 		2266.1
(-1.4) 		1995.1
(-1.8) 		1152.4
(-3) 		5421.7
(3) 		4269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1134
1 2 3 4 range
A 380.4
(-3)
P 		458
(-2.2)
P 		473.6
(-2)
P 		954.3
(3)
P 		574
B 337.2
(-3)
P 		462.5
(-2.3)
P 		422
(-2.6)
P 		1471.3
(3)
P 		1134
C 362.5
(-3)
P 		515.4
(-1.7)
P 		680.7
(-0.3)
P 		1076
(3)
P 		714
avr 360
(-3) 		478.6
(-2.1) 		525.4
(-1.8) 		1167.2
(3) 		807

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1762
1 2 3 4 5 6 range
A 185.4
(-3)
P 		540.8
(-1.1)
P 		542
(-1.1)
P 		531.7
(-1.2)
P 		300
(-2.4)
P 		1315.7
(3)
P 		1130
B 454.9
(-2.9)
P 		606.1
(-2.3)
P 		578.7
(-2.4)
P 		419.8
(-3)
P 		543.2
(-2.5)
P 		1947.4
(3)
P 		1528
C 509.8
(-2.4)
P 		482.9
(-2.5)
P 		490.4
(-2.5)
P 		463.3
(-2.6)
P 		364.4
(-3)
P 		1887.7
(3)
P 		1523
avr 383.4
(-3) 		543.3
(-2.3) 		537
(-2.3) 		471.6
(-2.6) 		402.5
(-2.9) 		1716.9
(3) 		1334
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174404_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20566
Sequence Type Exemplar sequence
Representative Public ID C57239 NCBI
Target Description C57239_rc /REP_DB=TREMBL Accession /GB=C57239 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk300h2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063376(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063376 NCBI Caenorhabditis elegans F14E5.5 (F14E5.5) mRNA, complete cds. 11/11 A 
SNAP00000024808 ENSEMBL cdna:SNAP chromosome:CEL140:II:8441095:8441923:1 11/11 None
GENEFINDER00000024809 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8439119:8441923:1 11/11 None
F14E5.5 ENSEMBL cdna:known chromosome:CEL140:II:8439108:8442051:1 gene:F14E5.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8441706-8441987(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14E5.5
Entrez Gene 174345 Entrez gene
SwissProt Q19462 EMBL-EBI
WormBase CE03208 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16042 lipid catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16298 lipase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91408 Hypothetical protein F14E5.5 [Caenorhabditis elegans] ref|NP_495777.1| F14E5.5 [Caenorhabditis elegans] 1.0E-164
blast CAE57648 Hypothetical protein CBG00637 [Caenorhabditis briggsae] 1.0E-150
blast CAA91408 Hypothetical protein F14E5.5 [Caenorhabditis elegans] ref|NP_495777.1| F14E5.5 [Caenorhabditis elegans] 2.0E-89
blast CAE57648 Hypothetical protein CBG00637 [Caenorhabditis briggsae] 7.0E-83
blast AAK31544 Hypothetical protein R07G3.2 [Caenorhabditis elegans] ref|NP_495596.3| R07G3.2 [Caenorhabditis elegans] 3.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR002918 EMBL-EBI Lipase, class 2 3.8E-109
Pfam IPR002918 EMBL-EBI Lipase, class 2 8.7E-81
Sequence
Target Sequence 
>C. ELEGANS:174404_S_AT
gaacatgttcgccatttattccgtagctgacgaagttattggactcagaaatacgtgtgg
agagatgtcctcaccattagaaggagccacttcaatcgtcaaaactagactaatgcatgg
agcagttattggtggaactacagaagatcaatggagggctcttcaaacctcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACATGTTCGCCATTTATTCCGTA 521 351 52 Antisense
CCGTAGCTGACGAAGTTATTGGACT 555 263 72 Antisense
GTTATTGGACTCAGAAATACGTGTG 67 447 86 Antisense
ACGTGTGGAGAGATGTCCTCACCAT 35 95 104 Antisense
GGAGAGATGTCCTCACCATTAGAAG 331 517 110 Antisense
GTCCTCACCATTAGAAGGAGCCACT 146 477 118 Antisense
AAGGAGCCACTTCAATCGTCAAAAC 612 159 132 Antisense
AAAACTAGACTAATGCATGGAGCAG 372 129 152 Antisense
ATGCATGGAGCAGTTATTGGTGGAA 639 41 164 Antisense
TGGTGGAACTACAGAAGATCAATGG 118 553 181 Antisense
CAATGGAGGGCTCTTCAAACCTCAT 622 181 200 Antisense
Sequence Source Affymetrix Proprietary Database