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[174401_at]

Description C36908_rc /REP_DB=TREMBL Accession /GB=C36908 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk469f8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 30      6grp_total > 18      4grp_total > 22      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1620
1 2 3 4 range
A 1343.7
(2.1)
P 		691.6
(-1.6)
P 		455.6
(-3)
P 		1494.9
(3)
P 		1039
B 1540.7
(1.3)
P 		604.3
(-2.2)
P 		379.5
(-3)
P 		1999.4
(3)
P 		1620
C 1569.7
(3)
P 		767.1
(-1.5)
P 		510
(-3)
P 		1381.7
(1.9)
P 		1060
avr 1484.7
(2.3) 		687.7
(-1.8) 		448.4
(-3) 		1625.3
(3) 		1177

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 561
1 2 3 4 5 6 range
A 994.4
(3)
P 		797.7
(0.4)
P 		665.1
(-1.3)
P 		539.5
(-3)
P 		821.9
(0.7)
P 		718.4
(-0.6)
P 		455
B 1100.4
(3)
P 		740.8
(-0.9)
P 		648.3
(-1.9)
P 		716.5
(-1.2)
P 		551.5
(-3)
P 		725.9
(-1.1)
P 		549
C 1075.1
(3)
P 		678.5
(-1.8)
P 		715
(-1.4)
P 		769.1
(-0.7)
P 		578.5
(-3)
P 		771.5
(-0.7)
P 		497
avr 1056.6
(3) 		739
(-1.7) 		676.1
(-2.6) 		675
(-2.6) 		650.6
(-3) 		738.6
(-1.7) 		406
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 432
1 2 3 4 range
A 489.3
(3)
P 		150.1
(-2.5)
P 		119.1
(-3)
P 		218.8
(-1.4)
P 		370
B 491.3
(3)
P 		149.2
(-2.1)
P 		87.9
(-3)
P 		426.1
(2)
P 		403
C 519.6
(3)
P 		220.5
(-2.2)
P 		177.7
(-3)
P 		233
(-2)
P 		342
avr 500.1
(3) 		173.3
(-2.3) 		128.2
(-3) 		292.6
(-0.3) 		372

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 262
1 2 3 4 5 6 range
A 183.3
(-1.1)
P 		283.7
(3)
P 		144.8
(-2.7)
P 		136.8
(-3)
P 		248
(1.5)
P 		174.9
(-1.4)
P 		147
B 382.9
(3)
P 		246.6
(-0.6)
P 		157.4
(-3)
P 		189.8
(-2.1)
P 		224.1
(-1.2)
P 		270.7
(0)
P 		226
C 398.5
(3)
P 		207
(-1.7)
P 		182
(-2.3)
P 		154.7
(-3)
P 		187.2
(-2.2)
P 		258.3
(-0.5)
P 		244
avr 321.6
(3) 		245.8
(0.2) 		161.4
(-3) 		160.4
(-3) 		219.8
(-0.8) 		234.6
(-0.2) 		161
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174401_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22183
Sequence Type Exemplar sequence
Representative Public ID C36908 NCBI
Target Description C36908_rc /REP_DB=TREMBL Accession /GB=C36908 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk469f8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171684(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171684 NCBI Caenorhabditis elegans Activator of G protein Signalling family member (ags-3) (ags-3) mRNA, complete cds. 11/11 None
F32A6.4a ENSEMBL cdna:known chromosome:CEL140:X:5289688:5298005:-1 gene:F32A6.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180801 3 1 NM_171684 174401_at (11), 188296_at (11)
NM_171951 188296_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5289753-5290050(-) 100.0 100.0
Public Domain and Genome References
Ensembl F32A6.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81387 Activator of g protein signalling protein 3, isoform a [Caenorhabditis elegans] ref|NP_741787.1| Activator of G protein Signalling family member (ags-3) [Caenorhabditis elegans] 0.0
blast AAL27247 Activator of g protein signalling protein 3, isoform b [Caenorhabditis elegans] ref|NP_741788.1| Activator of G protein Signalling family member (ags-3) [Caenorhabditis elegans] 0.0
blast CAE68728 Hypothetical protein CBG14654 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003109 EMBL-EBI GoLoco 2.2E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.6E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.6E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.4E-10
Pfam IPR001440 EMBL-EBI TPR repeat 9.1E-4
Pfam IPR001440 EMBL-EBI TPR repeat 0.096
Pfam IPR001440 EMBL-EBI TPR repeat 0.0076
Pfam IPR001440 EMBL-EBI TPR repeat 0.066
Pfam IPR001440 EMBL-EBI TPR repeat 3.3E-4
Sequence
Target Sequence 
>C. ELEGANS:174401_AT
ataccataccgcaaaagctttaaatttgatcgatttgccatttcttctcatttgctaaag
ttgtttcttttacccacttccaactccatcaaccacattttttgtttccatttcaaattt
tccagcgttccaaaaaaccgttgtctcctgattcatattcaaagtttataatgttcgttc
ctaaatatttattgaattcttattacattggatgccatcgttctcccctctaactaggtc
atctatttcaaatgcatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATACCATACCGCAAAAGCTTTAAAT 316 7 14 Antisense
AATTTGATCGATTTGCCATTTCTTC 270 175 36 Antisense
GCCATTTCTTCTCATTTGCTAAAGT 527 279 50 Antisense
AACTCCATCAACCACATTTTTTGTT 154 143 95 Antisense
AAAAAACCGTTGTCTCCTGATTCAT 464 129 145 Antisense
CGTTGTCTCCTGATTCATATTCAAA 26 249 152 Antisense
TCAAAGTTTATAATGTTCGTTCCTA 358 627 172 Antisense
AATGTTCGTTCCTAAATATTTATTG 643 163 183 Antisense
TTACATTGGATGCCATCGTTCTCCC 496 681 216 Antisense
CTCCCCTCTAACTAGGTCATCTATT 120 235 236 Antisense
AGGTCATCTATTTCAAATGCATCAA 707 57 249 Antisense
Sequence Source Affymetrix Proprietary Database