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[174382_s_at]

Description CEC309_rc /REP_DB=TREMBL Accession /GB=C07309 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk136g2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 18      4grp_highest_8_8 > 30      4grp_high_8_8 > 07      6grp_highest_nonorm > 61      6grp_total > 18      4grp_total > 01      6grp_highest_sub > De      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 485
1 2 3 4 range
A 466.1
(-1.2)
P 		359.3
(-3)
P 		447.7
(-1.5)
P 		717.1
(3)
P 		358
B 478.1
(-1.4)
P 		345.8
(-3)
P 		427.8
(-2)
P 		830.9
(3)
P 		485
C 431.7
(-3)
P 		482.9
(-1.4)
P 		464.6
(-2)
P 		622
(3)
P 		190
avr 458.6
(-1.9) 		396
(-3) 		446.7
(-2.1) 		723.3
(3) 		327

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 444
(3)
P 		414.7
(1.2)
P 		378.3
(-1.1)
P 		385.9
(-0.6)
P 		346.9
(-3)
P 		422.1
(1.6)
P 		97
B 464.5
(3)
P 		408.4
(0.9)
P 		414
(1.1)
P 		382.4
(-0.1)
P 		448.2
(2.4)
P 		306.7
(-3)
P 		158
C 549.1
(3)
P 		378.9
(-1)
P 		393
(-0.7)
P 		294.8
(-3)
P 		402
(-0.5)
P 		315.5
(-2.5)
P 		254
avr 485.9
(3) 		400.7
(-0.7) 		395.1
(-1) 		354.4
(-2.7) 		399
(-0.8) 		348.1
(-3) 		138
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 169.7
(3)
P 		78
(-3)
P 		117
(-0.4)
P 		105
(-1.2)
P 		92
B 152.5
(1.4)
P 		85.4
(-3)
P 		99.1
(-2.1)
P 		177.1
(3)
P 		92
C 142.9
(1)
P 		138.9
(0.6)
P 		161.9
(3)
P 		104.9
(-3)
P 		57
avr 155
(3) 		100.8
(-3) 		126
(-0.2) 		129
(0.1) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 81.8
(-3)
P 		147.5
(3)
P 		82.4
(-2.9)
P 		97.9
(-1.5)
P 		104.7
(-0.9)
P 		102.8
(-1.1)
P 		66
B 161.6
(1.5)
P 		135.9
(-0.4)
P 		100.5
(-3)
P 		101.3
(-2.9)
P 		182.1
(3)
P 		114.4
(-2)
P 		82
C 203.5
(3)
P 		115.6
(-0.7)
P 		100
(-1.3)
P 		59.3
(-3)
P 		130.1
(-0.1)
P 		105.6
(-1.1)
P 		144
avr 149
(3) 		133
(1.5) 		94.3
(-2.2) 		86.2
(-3) 		139
(2) 		107.6
(-1) 		63
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174382_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22109
Sequence Type Exemplar sequence
Representative Public ID CEC309 NCBI
Target Description CEC309_rc /REP_DB=TREMBL Accession /GB=C07309 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk136g2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029510(11), NM_001029508(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029510 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-2) (dgk-2) mRNA, complete cds. 11/11 None
NM_001029508 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-2) (dgk-2) mRNA, complete cds. 11/11 None
SNAP00000026673 ENSEMBL cdna:SNAP chromosome:CEL140:X:4495198:4496747:-1 11/11 None
GENEFINDER00000026676 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4495198:4504070:-1 11/11 None
F46H6.2c ENSEMBL cdna:known chromosome:CEL140:X:4494886:4497211:-1 gene:F46H6.2 11/11 A 
F46H6.2a ENSEMBL cdna:known chromosome:CEL140:X:4495198:4504126:-1 gene:F46H6.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180720 3 2 NM_001029508 174382_s_at (11), 189788_at (11)
NM_001029510 174381_at (11), 174382_s_at (11), 189788_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4494929-4495402(-) 100.0 100.0
Public Domain and Genome References
Ensembl F46H6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAP40526 Diacylglycerol kinase protein 2, isoform a [Caenorhabditis elegans] ref|NP_001024679.1| DiacylGlycerol Kinase family member (dgk-2) [Caenorhabditis elegans] 0.0
blast AAP40528 Diacylglycerol kinase protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024681.1| DiacylGlycerol Kinase family member (dgk-2) [Caenorhabditis elegans] 0.0
blast CAE68602 Hypothetical protein CBG14478 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.3E-34
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 4.0E-38
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.3E-34
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 4.9E-5
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.4E-6
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 4.0E-38
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 1.7E-31
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 4.0E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024679.1 1 9-26
F46H6.2a 1 9-26
GENEFINDER00000026676 1 9-26
Sequence
Target Sequence 
>C. ELEGANS:174382_S_AT
gtgttcaaggggaatcactcgttccctatgcaatcggatggtgaagcatgggtgaattcg
gccggaacagtgctaatctcccataagtgcaagactgcgatgctgaggaaagcggagaag
cagacgaccggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTTCAAGGGGAATCACTCGTTCC 545 467 15 Antisense
AAGGGGAATCACTCGTTCCCTATGC 620 159 21 Antisense
CACTCGTTCCCTATGCAATCGGATG 403 197 30 Antisense
GCAATCGGATGGTGAAGCATGGGTG 446 303 44 Antisense
AAGCATGGGTGAATTCGGCCGGAAC 626 149 58 Antisense
TCGGCCGGAACAGTGCTAATCTCCC 94 607 72 Antisense
AACAGTGCTAATCTCCCATAAGTGC 21 137 80 Antisense
ATCTCCCATAAGTGCAAGACTGCGA 418 33 90 Antisense
AAGTGCAAGACTGCGATGCTGAGGA 300 159 99 Antisense
GACTGCGATGCTGAGGAAAGCGGAG 468 375 107 Antisense
AAAGCGGAGAAGCAGACGACCGGAT 618 121 123 Antisense
Sequence Source Affymetrix Proprietary Database