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[174381_at]

Description CEC309_rc /REP_DB=TREMBL Accession /GB=C07309 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk136g2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 09      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 71      6grp_total > 19      4grp_total > 09      6grp_cgc > 01      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 649
1 2 3 4 range
A 353.1
(-3)
P 		527.1
(-0.8)
P 		415.5
(-2.2)
P 		818.7
(3)
P 		466
B 349.8
(-3)
P 		457.6
(1)
P 		438.1
(0.3)
P 		510.9
(3)
P 		161
C 460
(-2.3)
P 		386.5
(-3)
P 		514.9
(-1.7)
P 		998.4
(3)
P 		612
avr 387.6
(-3) 		457.1
(-1.9) 		456.2
(-1.9) 		776
(3) 		388

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 350
1 2 3 4 5 6 range
A 417.4
(1.4)
P 		375.4
(0.3)
P 		477.6
(3)
P 		388.6
(0.6)
P 		422.4
(1.5)
P 		250.5
(-3)
P 		227
B 430.4
(1.8)
P 		352.3
(-0.2)
P 		240.6
(-3)
P 		406.4
(1.2)
P 		341.1
(-0.5)
P 		478
(3)
P 		237
C 467.9
(3)
P 		320.2
(0.4)
P 		251.2
(-0.8)
P 		338
(0.7)
P 		442.4
(2.5)
P 		128.5
(-3)
P 		339
avr 438.6
(3) 		349.3
(-0.5) 		323.1
(-1.5) 		377.7
(0.6) 		402
(1.6) 		285.7
(-3) 		153
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 128.6
(3)
P 		114.4
(-1.3)
P 		108.6
(-3)
P 		119.8
(0.4)
P 		20
B 111.6
(2.3)
P 		113
(3)
P 		101.5
(-3)
P 		108.9
(0.9)
P 		12
C 152.3
(0.6)
P 		111.1
(-3)
P 		179.5
(3)
P 		168.3
(2)
P 		68
avr 130.8
(2.5) 		112.8
(-3) 		129.9
(2.3) 		132.3
(3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 76.9
(-1.7)
P 		133.5
(3)
P 		104
(0.6)
P 		98.5
(0.1)
P 		127.4
(2.5)
P 		61
(-3)
P 		73
B 149.8
(1.6)
P 		117.3
(-0.1)
P 		58.4
(-3)
P 		107.7
(-0.5)
P 		138.6
(1)
P 		178.3
(3)
P 		120
C 173.4
(3)
P 		97.7
(-0.5)
P 		63.9
(-2)
P 		68
(-1.8)
P 		143.2
(1.6)
P 		43
(-3)
P 		130
avr 133.4
(2.7) 		116.2
(1) 		75.4
(-3) 		91.4
(-1.4) 		136.4
(3) 		94.1
(-1.2) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174381_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22109
Sequence Type Exemplar sequence
Representative Public ID CEC309 NCBI
Target Description CEC309_rc /REP_DB=TREMBL Accession /GB=C07309 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk136g2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029510(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029510 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-2) (dgk-2) mRNA, complete cds. 11/11 None
F46H6.2c ENSEMBL cdna:known chromosome:CEL140:X:4494886:4497211:-1 gene:F46H6.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029508 1/11 Cross Hyb Matching Probes None
SNAP00000026673 1/11 Cross Hyb Matching Probes None
GENEFINDER00000026676 1/11 Cross Hyb Matching Probes None
F46H6.2a 1/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180720 3 1 NM_001029508 174382_s_at (11), 189788_at (11)
NM_001029510 174381_at (11), 174382_s_at (11), 189788_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4494929-4495402(-) 100.0 100.0
Public Domain and Genome References
Ensembl F46H6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAP40526 Diacylglycerol kinase protein 2, isoform a [Caenorhabditis elegans] ref|NP_001024679.1| DiacylGlycerol Kinase family member (dgk-2) [Caenorhabditis elegans] 0.0
blast AAP40528 Diacylglycerol kinase protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024681.1| DiacylGlycerol Kinase family member (dgk-2) [Caenorhabditis elegans] 0.0
blast T16376 hypothetical protein F46H6.2 - Caenorhabditis elegans 1.0E-170
Protein Domains
Database ID Description E-Value
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.3E-34
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 4.0E-38
Sequence
Target Sequence 
>C. ELEGANS:174381_AT
gacgaccggatgtgggatgttcttctaaagatcccctcacaattctcccatatgtatatt
gcagttttctattttttgcctccattttgaattccaattgtgatctccttctatagtact
cattttttcttgatttttatttgacttaaacatttccatgcgaattttttctatatattt
gttctgtttttccttcatctgactgtgtttttggatcaattcatgtttgtcgaaattctt
tctttttcagaatttcgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGACCGGATGTGGGATGTTCTTC 451 377 137 Antisense
GGATGTTCTTCTAAAGATCCCCTCA 548 513 151 Antisense
CCCTCACAATTCTCCCATATGTATA 509 259 170 Antisense
TTGCCTCCATTTTGAATTCCAATTG 467 701 212 Antisense
AATTCCAATTGTGATCTCCTTCTAT 132 179 226 Antisense
GATCTCCTTCTATAGTACTCATTTT 488 419 238 Antisense
AACATTTCCATGCGAATTTTTTCTA 697 135 285 Antisense
TTTCTATATATTTGTTCTGTTTTTC 495 671 304 Antisense
TTTTCCTTCATCTGACTGTGTTTTT 291 677 324 Antisense
TTCATGTTTGTCGAAATTCTTTCTT 230 689 356 Antisense
TTCTTTCTTTTTCAGAATTTCGCCA 250 687 372 Antisense
Sequence Source Affymetrix Proprietary Database