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[174371_at]

Description CEC919_rc /REP_DB=TREMBL Accession /GB=C07919 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk168f6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 13      4grp_high_8_8 > 30      6grp_highest_nonorm > 73      6grp_total > 73      4grp_total > 25      6grp_highest_sub > 04      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 255
1 2 3 4 range
A 438
(-2.6)
P 		557.1
(0.4)
P 		423.6
(-3)
P 		660.2
(3)
P 		237
B 404.8
(-3)
P 		455.2
(-1.5)
P 		610.4
(3)
P 		600.8
(2.7)
P 		206
C 413.9
(-3)
P 		491.2
(0.1)
P 		517.4
(1.1)
P 		564.2
(3)
P 		150
avr 418.9
(-3) 		501.2
(-0.4) 		517.1
(0.1) 		608.4
(3) 		190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 346
1 2 3 4 5 6 range
A 463.5
(-2.6)
P 		452.6
(-2.9)
P 		673.4
(3)
P 		448.1
(-3)
P 		571.8
(0.3)
P 		492.6
(-1.8)
P 		225
B 492.8
(-3)
P 		574.5
(-1.4)
P 		647.9
(0.1)
P 		794.5
(3)
P 		591.2
(-1)
P 		635.8
(-0.2)
P 		302
C 531.8
(-1)
P 		455.1
(-3)
P 		482.3
(-2.3)
P 		681.5
(3)
P 		601.3
(0.9)
P 		468.4
(-2.6)
P 		226
avr 496
(-2.9) 		494.1
(-3) 		601.2
(1.4) 		641.4
(3) 		588.1
(0.8) 		532.3
(-1.4) 		147
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 159.5
(3)
P 		120.9
(-0.7)
P 		110.7
(-1.7)
P 		96.6
(-3)
P 		63
B 129.1
(0.5)
P 		112.4
(-3)
P 		141.4
(3)
P 		128
(0.2)
P 		29
C 137
(-0)
P 		141.2
(0.2)
P 		180.3
(3)
P 		95.1
(-3)
P 		85
avr 141.9
(2.6) 		124.8
(-0.1) 		144.1
(3) 		106.6
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 155
1 2 3 4 5 6 range
A 85.4
(-3)
P 		161
(2.2)
P 		146.6
(1.2)
P 		113.6
(-1.1)
P 		172.5
(3)
P 		119.9
(-0.6)
P 		87
B 171.5
(-2)
P 		191.2
(-0.5)
P 		157.3
(-3)
P 		210.4
(0.8)
P 		240.2
(3)
P 		237.1
(2.8)
P 		83
C 197.1
(3)
P 		138.9
(-1.7)
P 		122.8
(-3)
P 		137
(-1.9)
P 		194.6
(2.8)
P 		156.8
(-0.3)
P 		74
avr 151.3
(-2.1) 		163.7
(-0.9) 		142.2
(-3) 		153.7
(-1.9) 		202.4
(3) 		171.3
(-0.1) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174371_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20898
Sequence Type Exemplar sequence
Representative Public ID CEC919 NCBI
Target Description CEC919_rc /REP_DB=TREMBL Accession /GB=C07919 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk168f6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027561(11), NM_001027560(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027561 NCBI Caenorhabditis elegans TOllisH (Tolloid and BMP-1 family) family member (toh-2) (toh-2) mRNA, complete cds. 11/11 None
NM_001027560 NCBI Caenorhabditis elegans TOllisH (Tolloid and BMP-1 family) family member (toh-2) (toh-2) mRNA, complete cds. 11/11 None
R151.5a ENSEMBL cdna:known chromosome:CEL140:III:7202585:7207555:1 gene:R151.5 11/11 A 
R151.5b ENSEMBL cdna:known chromosome:CEL140:III:7202586:7207572:1 gene:R151.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7207120-7207533(+) 100.0 100.0
Public Domain and Genome References
Ensembl R151.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAT92065 Tollish (tolloid and bmp-1 family) protein 2, isoform b [Caenorhabditis elegans] ref|NP_001022732.1| TOllisH (Tolloid and BMP-1 family) family member (toh-2) [Caenorhabditis elegans] 0.0
blast AAK29859 Tollish (tolloid and bmp-1 family) protein 2, isoform a [Caenorhabditis elegans] ref|NP_001022731.1| TOllisH (Tolloid and BMP-1 family) family member (toh-2) [Caenorhabditis elegans] sp|P98060|NAS35_CAEEL Zinc metalloproteinase nas-35 precursor (Nematode astacin 35) (Dumpy protein 31) (Tollish protein 2) 0.0
blast T16761 hypothetical protein R151.5 - Caenorhabditis elegans 0.0
blast CAE70154 Hypothetical protein CBG16621 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 6.6E-112
Pfam IPR000859 EMBL-EBI CUB 1.3E-17
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 3.1E-13
Sequence
Target Sequence 
>C. ELEGANS:174371_AT
gcagatgtgtccggtgatcaccaatttgtatccattatttcaattccatattttatcctg
taacctacagaactcttacgaagctacgggccaattcctaatcaaaccaaattgttataa
tccttattttcactgtttcatttttcttcttctaaattaaataccacct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGATGTGTCCGGTGATCACCAAT 438 311 176 Antisense
GGTGATCACCAATTTGTATCCATTA 139 501 188 Antisense
TTGTATCCATTATTTCAATTCCATA 51 707 201 Antisense
ATTCCATATTTTATCCTGTAACCTA 250 7 218 Antisense
CCTGTAACCTACAGAACTCTTACGA 280 265 232 Antisense
AGAACTCTTACGAAGCTACGGGCCA 661 71 244 Antisense
AAGCTACGGGCCAATTCCTAATCAA 709 147 256 Antisense
GGGCCAATTCCTAATCAAACCAAAT 592 491 263 Antisense
ATAATCCTTATTTTCACTGTTTCAT 565 25 292 Antisense
CTTATTTTCACTGTTTCATTTTTCT 201 221 298 Antisense
TCTTCTTCTAAATTAAATACCACCT 452 615 320 Antisense
Sequence Source Affymetrix Proprietary Database