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[174365_s_at] DNA polymerase

Description C55196_rc /REP_DB=TREMBL Accession /GB=C55196 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk430b4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      6grp_highest_nonorm > 91      6grp_total > 76      4grp_total > 15      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 144.3
(1.3)
P 		36.5
(-3)
A 		45.3
(-2.6)
A 		186.8
(3)
P 		150
B 120.2
(2.3)
P 		36.4
(-3)
A 		73.3
(-0.7)
P 		131.1
(3)
P 		95
C 140.1
(2.7)
P 		30.1
(-3)
A 		77.8
(-0.5)
P 		146.5
(3)
P 		116
avr 134.9
(2) 		34.3
(-3) 		65.5
(-1.4) 		154.8
(3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 415
1 2 3 4 5 6 range
A 87.3
(-3)
P 		131.6
(-2.4)
P 		217.3
(-1.1)
P 		164.8
(-1.9)
P 		231.3
(-0.9)
P 		497.6
(3)
P 		410
B 121.1
(-3)
P 		155.7
(-2.4)
P 		147.1
(-2.6)
P 		236
(-1.1)
P 		162.3
(-2.3)
P 		486.8
(3)
P 		366
C 119.4
(-2.4)
P 		147.7
(-2)
P 		82.7
(-3)
P 		226.6
(-0.7)
P 		148.1
(-2)
P 		458.9
(3)
P 		376
avr 109.3
(-3) 		145
(-2.4) 		149
(-2.4) 		209.1
(-1.4) 		180.6
(-1.8) 		481.1
(3) 		372
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 52.5
(3)
P 		7.9
(-3)
A 		11.8
(-2.5)
A 		27.3
(-0.4)
P 		45
B 38.3
(3)
P 		9
(-3)
A 		17
(-1.4)
P 		27.9
(0.9)
P 		29
C 46.4
(3)
P 		8.7
(-3)
A 		27.1
(-0.1)
P 		24.7
(-0.5)
P 		38
avr 45.7
(3) 		8.5
(-3) 		18.6
(-1.4) 		26.6
(-0.1) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 165
1 2 3 4 5 6 range
A 16.1
(-3)
P 		46.8
(-1.2)
P 		47.3
(-1.2)
P 		41.8
(-1.5)
P 		69.8
(0.1)
P 		121.2
(3)
P 		105
B 42.1
(-2.7)
P 		51.8
(-2.3)
P 		35.7
(-3)
P 		62.5
(-1.9)
P 		65.9
(-1.8)
P 		181.5
(3)
P 		146
C 44.3
(-1.9)
P 		45.1
(-1.9)
P 		21
(-3)
P 		45.6
(-1.9)
P 		47.9
(-1.8)
P 		153.6
(3)
P 		133
avr 34.2
(-3) 		47.9
(-2.3) 		34.7
(-3) 		50
(-2.2) 		61.2
(-1.6) 		152.1
(3) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174365_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21182
Sequence Type Exemplar sequence
Representative Public ID C55196 NCBI
Target Description C55196_rc /REP_DB=TREMBL Accession /GB=C55196 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk430b4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065849(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065849 NCBI Caenorhabditis elegans W03A3.2 (W03A3.2) mRNA, complete cds. 11/11 A 
GENEFINDER00000017292 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5791228:5798866:-1 11/11 None
W03A3.2 ENSEMBL cdna:known chromosome:CEL140:III:5791120:5797852:-1 gene:W03A3.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017286 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5791135-5791409(-) 100.0 100.0
Public Domain and Genome References
Gene Title DNA polymerase
Ensembl W03A3.2
Entrez Gene 175810 Entrez gene
WormBase CE38982 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.17880.1.S1_S_AT similar to DNA polymerase theta (DNA polymerase eta) cfa
DROSOPHILA_2:1635214_AT mutagen-sensitive 308 dm
DROSGENOME1:142745_AT mutagen-sensitive 308 dm
CHICKEN:GGA.18439.1.S1_S_AT similar to DNA polymerase theta isoform 1; polymerase (DNA-directed), theta gga
CHICKEN:GGAAFFX.9459.1.S1_S_AT similar to DNA polymerase theta isoform 1; polymerase (DNA-directed), theta gga
CHICKEN:GGAAFFX.9473.1.S1_S_AT similar to DNA polymerase theta isoform 1; polymerase (DNA-directed), theta gga
CHICKEN:GGAAFFX.9474.1.S1_S_AT similar to DNA polymerase theta isoform 1; polymerase (DNA-directed), theta gga
HG-U133_PLUS_2:207746_AT polymerase (DNA directed), theta hs
HG-U133A:207746_AT polymerase (DNA directed), theta hs
HG-U133A_2:207746_AT polymerase (DNA directed), theta hs
HG-U133_PLUS_2:219510_AT polymerase (DNA directed), theta hs
HG-U133A:219510_AT polymerase (DNA directed), theta hs
HG-FOCUS:219510_AT polymerase (DNA directed), theta hs
HG-U133A_2:219510_AT polymerase (DNA directed), theta hs
U133_X3P:G7662545_3P_AT polymerase (DNA directed), theta hs
U133_X3P:G5729983_3P_AT polymerase (DNA directed), theta hs
HG-U95E:89003_AT polymerase (DNA directed), theta hs
HG-U95D:86387_AT Polymerase (DNA directed), theta hs
MOE430B:1453551_AT polymerase (DNA directed), theta mm
MOUSE430_2:1453551_AT polymerase (DNA directed), theta mm
Protein Similarities
Method ID Description E-Value
blast NP_498250 W03A3.2 [Caenorhabditis elegans] 0.0
blast CAE64393 Hypothetical protein CBG09083 [Caenorhabditis briggsae] 1.0E-161
blast CAE64393 Hypothetical protein CBG09083 [Caenorhabditis briggsae] 1.0E-139
blast O52225 DNA polymerase I, thermostable (TFI polymerase 1) gb|AAC46079.1| thermostable DNA polymerase [Thermus filiformis] 2.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR001098 EMBL-EBI DNA-directed DNA polymerase 5.0E-56
Pfam IPR001098 EMBL-EBI DNA-directed DNA polymerase 5.0E-56
Sequence
Target Sequence 
>C. ELEGANS:174365_S_AT
acaaatcgtcctaaccatccacgatgaagttctagtcgaatgtccagaaatccacgtggc
agcagcttccgaatctattgaaaattgcatgcaaaatgctctttctcatttattacgagt
tccaatgcgggtttcaatgaaaacgggacgatcatgggctgatttaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAAATCGTCCTAACCATCCACGAT 133 111 24 Antisense
GTCCTAACCATCCACGATGAAGTTC 316 477 31 Antisense
GATGAAGTTCTAGTCGAATGTCCAG 308 413 46 Antisense
TGGCAGCAGCTTCCGAATCTATTGA 176 547 80 Antisense
GCAGCTTCCGAATCTATTGAAAATT 674 313 85 Antisense
GCATGCAAAATGCTCTTTCTCATTT 495 309 110 Antisense
TTTCTCATTTATTACGAGTTCCAAT 341 671 125 Antisense
ATTACGAGTTCCAATGCGGGTTTCA 165 9 135 Antisense
CGAGTTCCAATGCGGGTTTCAATGA 389 249 139 Antisense
GAAAACGGGACGATCATGGGCTGAT 235 351 162 Antisense
GGGACGATCATGGGCTGATTTAAAG 289 493 168 Antisense
Sequence Source Affymetrix Proprietary Database