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[174360_at] Cuticulin precursor

Description C32640_rc /REP_DB=TREMBL Accession /GB=C32640 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk334a2 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 46      6grp_total > 47      4grp_total > 52      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 76
(-1.5)
P 		131.5
(2.7)
P 		136
(3)
P 		55.6
(-3)
A 		80
B 103.8
(2.9)
P 		105.9
(3)
P 		76.4
(0.9)
P 		21.3
(-3)
A 		85
C 129.5
(2.2)
P 		147.4
(3)
P 		85.9
(0.2)
P 		14.6
(-3)
A 		133
avr 103.1
(1.5) 		128.3
(3) 		99.4
(1.2) 		30.5
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 119
1 2 3 4 5 6 range
A 182
(3)
P 		101.9
(-3)
P 		145.6
(0.3)
P 		105.4
(-2.7)
A 		104.8
(-2.8)
P 		134.6
(-0.6)
P 		80
B 156.9
(2.4)
P 		112.2
(-0.6)
P 		90
(-2.1)
P 		143.5
(1.5)
P 		77.1
(-3)
P 		165.3
(3)
P 		88
C 125.4
(-0.3)
P 		78.7
(-2.7)
P 		97.4
(-1.7)
P 		191
(3)
P 		71.8
(-3)
P 		135.1
(0.2)
P 		119
avr 154.8
(3) 		97.6
(-1.9) 		111
(-0.7) 		146.6
(2.3) 		84.6
(-3) 		145
(2.2) 		70
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 27.7
(1.3)
P 		28.5
(1.5)
P 		35.5
(3)
P 		8.1
(-3)
A 		27
B 33.1
(3)
P 		26.2
(1.6)
P 		17.7
(-0.2)
P 		4.5
(-3)
A 		29
C 42.9
(3)
P 		42.4
(2.9)
P 		29.9
(1.1)
P 		2.5
(-3)
A 		40
avr 34.6
(3) 		32.4
(2.6) 		27.7
(1.6) 		5
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 33.5
(1.3)
P 		36.2
(3)
P 		31.7
(0.2)
P 		26.7
(-3)
A 		31.6
(0.1)
P 		32.8
(0.9)
P 		10
B 54.6
(1.9)
P 		37.3
(-0.7)
P 		21.8
(-3)
P 		38
(-0.6)
P 		31.3
(-1.6)
P 		61.7
(3)
P 		40
C 46.5
(3)
P 		24
(-2.8)
P 		24.8
(-2.6)
P 		38.4
(0.9)
P 		23.2
(-3)
P 		45.2
(2.7)
P 		23
avr 44.9
(2.5) 		32.5
(-1.1) 		26.1
(-3) 		34.4
(-0.6) 		28.7
(-2.2) 		46.6
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174360_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20777
Sequence Type Exemplar sequence
Representative Public ID C32640 NCBI
Target Description C32640_rc /REP_DB=TREMBL Accession /GB=C32640 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk334a2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062895(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062895 NCBI Caenorhabditis elegans K06A1.3 (K06A1.3) mRNA, complete cds. 11/11 None
K06A1.3 ENSEMBL cdna:known chromosome:CEL140:II:6441375:6443868:1 gene:K06A1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6443574-6443855(+) 91.1 100.0
Public Domain and Genome References
Gene Title Cuticulin precursor
Ensembl K06A1.3
Entrez Gene 174065 Entrez gene
SwissProt Q09586 EMBL-EBI
WormBase CE32057 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24298 Hypothetical protein K06A1.3 [Caenorhabditis elegans] ref|NP_495296.2| K06A1.3 [Caenorhabditis elegans] 0.0
blast CAE59324 Hypothetical protein CBG02666 [Caenorhabditis briggsae] 1.0E-176
blast CAB60411 Hypothetical protein Y53H1B.1 [Caenorhabditis elegans] ref|NP_492900.1| Y53H1B.1 [Caenorhabditis elegans] 5.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 5.4E-32
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495296.2 1 453-475
K06A1.3 1 453-475
Sequence
Target Sequence 
>C. ELEGANS:174360_AT
ttcaatttccataatcctcagaagacctttccgttgcgtgtaaatgtttccccaaaatcc
cttcttcaaatgctttattctgttctcatttcatcagccttacatattttcctgccaaaa
tgggttgttttcgaataattttcctattaattataacaactattccatctttttgttttt
gatttgtgatttctgaatgatatgttatctccgtgccaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAATTTCCATAATCCTCAGAAGA 418 685 13 Antisense
TAATCCTCAGAAGACCTTTCCGTTG 93 635 24 Antisense
AGACCTTTCCGTTGCGTGTAAATGT 685 69 35 Antisense
TGCGTGTAAATGTTTCCCCAAAATC 408 579 47 Antisense
CCAAAATCCCTTCTTCAAATGCTTT 205 257 64 Antisense
TTCAAATGCTTTATTCTGTTCTCAT 130 687 77 Antisense
ATTTCATCAGCCTTACATATTTTCC 366 15 100 Antisense
CAGCCTTACATATTTTCCTGCCAAA 512 201 107 Antisense
TCCTGCCAAAATGGGTTGTTTTCGA 195 593 122 Antisense
ACTATTCCATCTTTTTGTTTTTGAT 173 103 171 Antisense
ATGATATGTTATCTCCGTGCCAATT 194 47 209 Antisense
Sequence Source Affymetrix Proprietary Database