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[174354_at]

Description C34260_rc /REP_DB=TREMBL Accession /GB=C34260 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk394a6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 27      4grp_highest_8_8 > 34      4grp_high_8_8 > 58      6grp_highest_nonorm > 60      6grp_total > 27      4grp_total > 62      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > Cu      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 583
1 2 3 4 range
A 748.2
(3)
P 		353.7
(-1.1)
P 		548.2
(0.9)
P 		164.9
(-3)
P 		583
B 706.2
(3)
P 		488.5
(0.3)
P 		560.4
(1.2)
P 		217.7
(-3)
P 		489
C 693.3
(3)
P 		558.5
(1.5)
P 		617.5
(2.1)
P 		166.3
(-3)
P 		527
avr 715.9
(3) 		466.9
(0.2) 		575.4
(1.4) 		183
(-3) 		533

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 529
1 2 3 4 5 6 range
A 518.7
(3)
P 		428
(1.3)
P 		204.3
(-3)
P 		251.1
(-2.1)
P 		241.3
(-2.3)
P 		314.9
(-0.9)
P 		314
B 724.6
(3)
P 		553.9
(1.1)
P 		300.8
(-1.8)
P 		195.5
(-3)
P 		227
(-2.6)
P 		268.3
(-2.2)
P 		529
C 721.6
(3)
P 		447.4
(-0.4)
P 		242.5
(-2.9)
P 		256.5
(-2.7)
P 		233.2
(-3)
P 		352.1
(-1.5)
P 		488
avr 655
(3) 		476.4
(0.5) 		249.2
(-2.8) 		234.4
(-3) 		233.8
(-3) 		311.8
(-1.9) 		421
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 248
1 2 3 4 range
A 272.4
(3)
P 		76.8
(-1.7)
P 		143.3
(-0.1)
P 		24.1
(-3)
P 		248
B 225.2
(3)
P 		120.7
(-0.5)
P 		129.9
(-0.2)
P 		46.4
(-3)
P 		179
C 229.5
(3)
P 		160.6
(0.9)
P 		215.2
(2.6)
P 		28
(-3)
P 		202
avr 242.4
(3) 		119.4
(-0.5) 		162.8
(0.7) 		32.8
(-3) 		210

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 95.6
(-0.2)
P 		152.2
(3)
P 		44.5
(-3)
P 		63.7
(-1.9)
P 		72.8
(-1.4)
P 		76.7
(-1.2)
P 		108
B 252.1
(3)
P 		184.4
(1)
P 		73.1
(-2.4)
P 		51.8
(-3)
P 		92.2
(-1.8)
P 		100
(-1.6)
P 		200
C 267.5
(3)
P 		136.5
(-0.6)
P 		61.7
(-2.7)
P 		51.6
(-3)
P 		75.5
(-2.3)
P 		117.9
(-1.2)
P 		216
avr 205.1
(3) 		157.7
(1.1) 		59.8
(-2.8) 		55.7
(-3) 		80.2
(-2) 		98.2
(-1.3) 		149
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174354_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20544
Sequence Type Exemplar sequence
Representative Public ID C34260 NCBI
Target Description C34260_rc /REP_DB=TREMBL Accession /GB=C34260 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk394a6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027088(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027088 NCBI Caenorhabditis elegans Initiation Factor 4G (eIF4G) family member (ifg-1) (ifg-1) mRNA, complete cds. 11/11 None
M110.4a ENSEMBL cdna:known chromosome:CEL140:II:8217154:8222293:1 gene:M110.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174322 2 1 NM_001027088 174354_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8221827-8222111(+) 100.0 100.0
Public Domain and Genome References
Ensembl M110.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90261 Hypothetical protein M110.4a [Caenorhabditis elegans] ref|NP_001022259.1| Initiation Factor 4G (eIF4G) family member (ifg-1) [Caenorhabditis elegans] 0.0
blast CAE47470 Hypothetical protein M110.4b [Caenorhabditis elegans] ref|NP_001022260.1| Initiation Factor 4G (eIF4G) family member (ifg-1) [Caenorhabditis elegans] 0.0
blast CAE57703 Hypothetical protein CBG00709 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003890 EMBL-EBI Initiation factor eIF-4 gamma, middle 1.8E-58
Sequence
Target Sequence 
>C. ELEGANS:174354_AT
gcttgttttcgtgcttaaagggtcttttctaagagacggtcgaaaagtcgctgaattttt
tcggagtgctggaatttcgttgaattgcatcgaatttatcgtaaaacgaagacttttgac
tagctcttagcttcatttcttttccaaatttcctaattgtgtgtaagtcagtgccttcgg
tctgattcctggatcttctagaaattgtgacaattggttaattttaatatcctctcatca
gcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTGTTTTCGTGCTTAAAGGGTCT 393 305 17 Antisense
GAGACGGTCGAAAAGTCGCTGAATT 578 387 49 Antisense
GTCGCTGAATTTTTTCGGAGTGCTG 428 473 63 Antisense
TTCGGAGTGCTGGAATTTCGTTGAA 390 693 76 Antisense
AGACTTTTGACTAGCTCTTAGCTTC 615 71 126 Antisense
CTCTTAGCTTCATTTCTTTTCCAAA 62 231 140 Antisense
TCATTTCTTTTCCAAATTTCCTAAT 22 619 149 Antisense
ATTTCCTAATTGTGTGTAAGTCAGT 611 15 164 Antisense
GTAAGTCAGTGCCTTCGGTCTGATT 151 463 179 Antisense
CGGTCTGATTCCTGGATCTTCTAGA 669 243 194 Antisense
TAATTTTAATATCCTCTCATCAGCC 320 635 235 Antisense
Sequence Source Affymetrix Proprietary Database