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[174337_at]

Description C52538_rc /REP_DB=TREMBL Accession /GB=C52538 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk250f6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 60      6grp_total > 06      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 530
1 2 3 4 range
A 908.5
(3)
P 		587.7
(-0.6)
P 		378.4
(-3)
P 		483.6
(-1.8)
P 		530
B 877.2
(3)
P 		575.7
(-1.5)
P 		472.7
(-3)
P 		621
(-0.8)
P 		405
C 882.1
(3)
P 		635.2
(-0.5)
P 		460.9
(-3)
P 		688
(0.2)
P 		421
avr 889.3
(3) 		599.5
(-0.8) 		437.3
(-3) 		597.5
(-0.9) 		452

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 418
1 2 3 4 5 6 range
A 411.3
(2.2)
P 		442.4
(3)
P 		391.9
(1.7)
P 		245.7
(-2.1)
P 		368.6
(1.1)
P 		212.2
(-3)
P 		230
B 629.7
(3)
P 		480.3
(0.3)
P 		344
(-2.2)
P 		339.6
(-2.2)
P 		311.2
(-2.8)
P 		297.8
(-3)
P 		332
C 579.9
(3)
P 		377.7
(-0.8)
P 		329.1
(-1.7)
P 		277.4
(-2.6)
P 		279.6
(-2.6)
P 		257.1
(-3)
P 		323
avr 540.3
(3) 		433.5
(0.7) 		355
(-0.9) 		287.6
(-2.3) 		319.8
(-1.6) 		255.7
(-3) 		285
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 330.8
(3)
P 		127.6
(-1.7)
P 		98.9
(-2.4)
P 		70.8
(-3)
P 		260
B 279.7
(3)
P 		142.2
(-1.8)
P 		109.5
(-3)
P 		132.3
(-2.2)
P 		170
C 292
(3)
P 		182.6
(-0.7)
P 		160.6
(-1.5)
P 		116
(-3)
P 		176
avr 300.8
(3) 		150.8
(-1.6) 		123
(-2.5) 		106.4
(-3) 		194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 167
1 2 3 4 5 6 range
A 75.8
(-1.6)
P 		157.3
(3)
P 		85.3
(-1.1)
P 		62.3
(-2.4)
P 		111.2
(0.4)
P 		51.7
(-3)
P 		106
B 219.1
(3)
P 		159.9
(0.4)
P 		83.5
(-3)
P 		90
(-2.7)
P 		126.4
(-1.1)
P 		111
(-1.8)
P 		136
C 215
(3)
P 		115.2
(-0.8)
P 		83.8
(-1.9)
P 		55.8
(-3)
P 		90.5
(-1.7)
P 		86.1
(-1.9)
P 		159
avr 170
(3) 		144.1
(1.5) 		84.2
(-2.1) 		69.4
(-3) 		109.4
(-0.6) 		82.9
(-2.2) 		101
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174337_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21214
Sequence Type Exemplar sequence
Representative Public ID C52538 NCBI
Target Description C52538_rc /REP_DB=TREMBL Accession /GB=C52538 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk250f6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027502(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027502 NCBI Caenorhabditis elegans NADC (Na+-coupled dicarboxylate transporter) family member (nac-3) (nac-3) mRNA, complete cds. 11/11 None
K08E5.2a ENSEMBL cdna:known chromosome:CEL140:III:10152682:10157812:1 gene:K08E5.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176429 2 1 NM_001027502 174337_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10157447-10157723(+) 100.0 100.0
Public Domain and Genome References
Ensembl K08E5.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83225 Hypothetical protein K08E5.2a [Caenorhabditis elegans] ref|NP_001022673.1| NADC (Na+-coupled dicarboxylate transporter) family member (nac-3) [Caenorhabditis elegans] gb|AAM11894.1| high-affinity plasma membrane transporter INDY2 [Caenorhabditis elegans] sp|Q21339|NAD3_CAEEL Sodium-dependent high-affinity dicarboxylate transporter 3 (Na(+)/dicarboxylate cotransporter 3) (NaDC-3) (ceNaDC3) 0.0
blast CAE64963 Hypothetical protein CBG09797 [Caenorhabditis briggsae] 0.0
blast G88575 protein K08E5.2 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 1.5E-39
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 5.1E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022673.1 12 51-70,90-112,125-139,162-179,220-242,266-288,318-337,352-371,401-420,450-472,484-506,521-543
K08E5.2a 12 51-70,90-112,125-139,162-179,220-242,266-288,318-337,352-371,401-420,450-472,484-506,521-543
Sequence
Target Sequence 
>C. ELEGANS:174337_AT
gggtattctgattcaaccttttttggtcatcctcttcctttgcctcttcccacacccaga
accatttctcaaacatctacatgacttagtcattcttgttacgggaacttcagtcaaatc
acacttgttgcaaacgctcaccccatcatcaaaaaaaacttgaaatttcaaaaaacaact
taacagatcttctattacaaaccgtttatttttcttttcttgctgtgttatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGTATTCTGATTCAACCTTTTTTG 407 497 13 Antisense
TTCAACCTTTTTTGGTCATCCTCTT 12 685 24 Antisense
ACACCCAGAACCATTTCTCAAACAT 451 109 64 Antisense
TCTCAAACATCTACATGACTTAGTC 239 613 79 Antisense
TGACTTAGTCATTCTTGTTACGGGA 502 569 94 Antisense
CATTCTTGTTACGGGAACTTCAGTC 601 215 103 Antisense
GAACTTCAGTCAAATCACACTTGTT 104 351 117 Antisense
AATCACACTTGTTGCAAACGCTCAC 29 171 129 Antisense
AAAACAACTTAACAGATCTTCTATT 455 129 184 Antisense
ACAGATCTTCTATTACAAACCGTTT 10 109 195 Antisense
ATTTTTCTTTTCTTGCTGTGTTATC 304 15 220 Antisense
Sequence Source Affymetrix Proprietary Database