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[174336_at]

Description C53391_rc /REP_DB=TREMBL Accession /GB=C53391 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk315c4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 91      6grp_high1_10_10 > 29      4grp_highest_8_8 > 44      4grp_high_8_8 > 16      6grp_highest_nonorm > 73      6grp_total > 29      4grp_total > 31      6grp_cgc > 03      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 624
1 2 3 4 range
A 854.5
(3)
P 		230.5
(-3)
P 		320.1
(-2.1)
P 		486.1
(-0.5)
P 		624
B 739.6
(3)
P 		358.9
(-1.8)
P 		263.6
(-3)
P 		643.8
(1.8)
P 		476
C 763.6
(3)
P 		323.6
(-3)
P 		387.4
(-2.1)
P 		526.3
(-0.2)
P 		440
avr 785.9
(3) 		304.3
(-3) 		323.7
(-2.8) 		552.1
(0.1) 		482

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 407
1 2 3 4 5 6 range
A 604
(2.3)
P 		601.2
(2.3)
P 		354.1
(-3)
P 		478.1
(-0.4)
P 		635.4
(3)
P 		506.8
(0.3)
P 		281
B 725.9
(3)
P 		622.4
(0.9)
P 		431.4
(-3)
P 		484
(-1.9)
P 		584.2
(0.1)
P 		590.8
(0.2)
P 		295
C 760.6
(3)
P 		697.7
(1.7)
P 		477.6
(-3)
P 		519.7
(-2.1)
P 		581.5
(-0.8)
P 		550.1
(-1.5)
P 		283
avr 696.8
(3) 		640.4
(1.8) 		421
(-3) 		493.9
(-1.4) 		600.4
(0.9) 		549.2
(-0.2) 		276
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 261
1 2 3 4 range
A 311.1
(3)
P 		50
(-3)
P 		83.7
(-2.2)
P 		71.2
(-2.5)
P 		261
B 235.9
(3)
P 		88.6
(-2.1)
P 		61.1
(-3)
P 		137.2
(-0.4)
P 		175
C 252.8
(3)
P 		93
(-2.8)
P 		135
(-1.3)
P 		88.7
(-3)
P 		164
avr 266.6
(3) 		77.2
(-3) 		93.3
(-2.5) 		99
(-2.3) 		189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 111.3
(-1.5)
P 		213.8
(3)
P 		77.1
(-3)
P 		121.3
(-1.1)
P 		191.7
(2)
P 		123.4
(-1)
P 		137
B 252.6
(3)
P 		207.2
(1.2)
P 		104.7
(-3)
P 		128.2
(-2)
P 		237.3
(2.4)
P 		220.3
(1.7)
P 		148
C 281.9
(3)
P 		212.9
(0.7)
P 		121.6
(-2.4)
P 		104.5
(-3)
P 		188.2
(-0.2)
P 		184.1
(-0.3)
P 		177
avr 215.3
(3) 		211.3
(2.8) 		101.1
(-3) 		118
(-2.1) 		205.7
(2.5) 		175.9
(0.9) 		114
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174336_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21501
Sequence Type Exemplar sequence
Representative Public ID C53391 NCBI
Target Description C53391_rc /REP_DB=TREMBL Accession /GB=C53391 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk315c4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027868(11), NM_001027867(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027868 NCBI Caenorhabditis elegans EXCretory canal abnormal family member (exc-5) (exc-5) mRNA, complete cds. 11/11 None
NM_001027867 NCBI Caenorhabditis elegans EXCretory canal abnormal family member (exc-5) (exc-5) mRNA, complete cds. 11/11 None
C33D9.1b ENSEMBL cdna:known chromosome:CEL140:IV:8799071:8804839:-1 gene:C33D9.1 11/11 None
C33D9.1a ENSEMBL cdna:known chromosome:CEL140:IV:8799074:8804839:-1 gene:C33D9.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8799145-8799425(-) 100.0 100.0
Public Domain and Genome References
Ensembl C33D9.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD30432 Hypothetical protein C33D9.1b [Caenorhabditis elegans] ref|NP_001023039.1| EXCretory canal abnormal family member (exc-5) [Caenorhabditis elegans] gb|AAL50361.1| Rho guanine nucleotide exchange factor FGD-1 [Caenorhabditis elegans] dbj|BAB43906.1| putative GDP-GTP exchange factor [Caenorhabditis elegans] 0.0
blast CAA92282 Hypothetical protein C33D9.1a [Caenorhabditis elegans] ref|NP_001023038.1| EXCretory canal abnormal family member (exc-5) [Caenorhabditis elegans] gb|AAL50355.1| putative Rho guanine nucleotide exchange factor FGD-1 [Caenorhabditis elegans] 0.0
blast CAE56377 Hypothetical protein CBG24054 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.8E-20
Pfam IPR000219 EMBL-EBI DH domain 5.7E-38
Sequence
Target Sequence 
>C. ELEGANS:174336_AT
gttatttcagtgttctattcttgtttttctctcaattcttattattcgttcgttgaaact
gtatccaaattttccgaactgattatattttttaaatacgttcatctcatctcatccctc
ttctctcactcactactaaaatcgtacgcaagtgcgttccgcctgaacacatatctgttc
tcgaattctagtcatcgtagaatctgaaattttgaactcctgcaatctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTATTTCAGTGTTCTATTCTTGTT 639 447 27 Antisense
TATTCTTGTTTTTCTCTCAATTCTT 61 665 42 Antisense
CAATTCTTATTATTCGTTCGTTGAA 635 179 59 Antisense
ATTCGTTCGTTGAAACTGTATCCAA 461 9 70 Antisense
ACTGTATCCAAATTTTCCGAACTGA 420 97 84 Antisense
TAAATACGTTCATCTCATCTCATCC 430 641 119 Antisense
CTTCTCTCACTCACTACTAAAATCG 346 221 146 Antisense
AATCGTACGCAAGTGCGTTCCGCCT 340 169 166 Antisense
CGTTCCGCCTGAACACATATCTGTT 595 245 181 Antisense
TATCTGTTCTCGAATTCTAGTCATC 436 657 198 Antisense
GAAATTTTGAACTCCTGCAATCTGA 517 363 232 Antisense
Sequence Source Affymetrix Proprietary Database