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[174330_at] phosphatidylserine synthase I

Description C31805_rc /REP_DB=TREMBL Accession /GB=C31805 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk345c1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 18      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 70      6grp_total > 06      4grp_total > 62      6grp_highest_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 326
1 2 3 4 range
A 486.4
(3)
P 		310.3
(-0.3)
P 		291
(-0.7)
P 		169.8
(-3)
P 		317
B 460.2
(3)
P 		290.2
(-1.2)
P 		314.5
(-0.6)
P 		216.4
(-3)
P 		244
C 447.5
(3)
P 		361.8
(1.2)
P 		378.8
(1.6)
P 		160.6
(-3)
P 		287
avr 464.7
(3) 		320.8
(-0.1) 		328.1
(0.1) 		182.3
(-3) 		282

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 295
1 2 3 4 5 6 range
A 432.7
(3)
P 		295.9
(0.2)
P 		226.4
(-1.2)
P 		137.7
(-3)
P 		322.8
(0.8)
P 		143.5
(-2.9)
P 		295
B 367.1
(3)
P 		280.4
(-0.4)
P 		241.5
(-1.9)
P 		249.6
(-1.6)
P 		214.3
(-3)
P 		231.3
(-2.3)
P 		153
C 385.7
(3)
P 		254.8
(-0.8)
P 		177.2
(-3)
P 		179.8
(-2.9)
P 		216.6
(-1.9)
P 		192.4
(-2.6)
P 		209
avr 395.2
(3) 		277
(-0.4) 		215
(-2.2) 		189
(-3) 		251.2
(-1.2) 		189.1
(-3) 		206
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 177.1
(3)
P 		67.4
(-1.3)
P 		76.1
(-1)
P 		24.9
(-3)
P 		152
B 146.8
(3)
P 		71.7
(-1.5)
P 		72.9
(-1.4)
P 		46.1
(-3)
P 		101
C 148.1
(3)
P 		104
(0.8)
P 		132
(2.2)
P 		27.1
(-3)
P 		121
avr 157.3
(3) 		81
(-0.7) 		93.7
(-0.1) 		32.7
(-3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 79.8
(0.8)
P 		105.2
(3)
P 		49.3
(-1.8)
P 		34.9
(-3)
P 		97.4
(2.3)
P 		34.9
(-3)
P 		70
B 127.7
(3)
P 		93.3
(0)
P 		58.6
(-3)
P 		66.1
(-2.3)
P 		87.1
(-0.5)
P 		86.3
(-0.6)
P 		69
C 142.9
(3)
P 		77.8
(-0.7)
P 		45.1
(-2.5)
P 		36.2
(-3)
P 		70.1
(-1.1)
P 		64.4
(-1.4)
P 		107
avr 116.8
(3) 		92.1
(0.9) 		51
(-2.6) 		45.7
(-3) 		84.9
(0.3) 		61.9
(-1.6) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174330_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22197
Sequence Type Exemplar sequence
Representative Public ID C31805 NCBI
Target Description C31805_rc /REP_DB=TREMBL Accession /GB=C31805 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk345c1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077272(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077272 NCBI Caenorhabditis elegans ZC506.3 (ZC506.3) mRNA, complete cds. 11/11 None
ZC506.3.3 ENSEMBL cdna:known chromosome:CEL140:X:9965425:9968498:1 gene:ZC506.3 11/11 None
ZC506.3.2 ENSEMBL cdna:known chromosome:CEL140:X:9965522:9968506:1 gene:ZC506.3 11/11 None
ZC506.3.1 ENSEMBL cdna:known chromosome:CEL140:X:9965529:9968506:1 gene:ZC506.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9968158-9968439(+) 100.0 100.0
Public Domain and Genome References
Gene Title phosphatidylserine synthase I
Ensembl ZC506.3
Entrez Gene 181211 Entrez gene
SwissProt Q09643 EMBL-EBI
WormBase CE01681 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.14908.1.S1_AT similar to Phosphatidylserine synthase I (PtdSer synthase-1) (PSS-1) (Serine-exchange enzyme I) cfa
DROSOPHILA_2:1628240_AT dm
DROSGENOME1:149137_AT dm
CHICKEN:GGAAFFX.10193.1.S1_S_AT phosphatidylserine synthase 1 gga
CHICKEN:GGAAFFX.21515.1.S1_S_AT phosphatidylserine synthase 1 gga
HG-U133A:201433_S_AT phosphatidylserine synthase 1 hs
HG-U133_PLUS_2:201433_S_AT phosphatidylserine synthase 1 hs
HG-FOCUS:201433_S_AT phosphatidylserine synthase 1 hs
HG-U133A_2:201433_S_AT phosphatidylserine synthase 1 hs
HG-U95AV2:37320_AT phosphatidylserine synthase 1 hs
HUGENEFL:D14694_AT phosphatidylserine synthase 1 hs
U133_X3P:G7662646_3P_A_AT phosphatidylserine synthase 1 hs
MOUSE430A_2:1441866_S_AT phosphatidylserine synthase 1 mm
MOE430A:1441866_S_AT phosphatidylserine synthase 1 mm
MOUSE430_2:1441866_S_AT phosphatidylserine synthase 1 mm
MU11KSUBB:MSA.12566.0_AT phosphatidylserine synthase 1 mm
MG-U74AV2:101931_AT phosphatidylserine synthase 1 mm
MG-U74AV2:161864_F_AT phosphatidylserine synthase 1 mm
MOE430A:1416372_AT phosphatidylserine synthase 1 mm
MOUSE430A_2:1416372_AT phosphatidylserine synthase 1 mm
MOUSE430_2:1416372_AT phosphatidylserine synthase 1 mm
MOE430A:1449739_AT Phosphatidylserine synthase 1, mRNA (cDNA clone MGC:5814 IMAGE:3501141) mm
MOUSE430A_2:1449739_AT Phosphatidylserine synthase 1, mRNA (cDNA clone MGC:5814 IMAGE:3501141) mm
MOUSE430_2:1449739_AT Phosphatidylserine synthase 1, mRNA (cDNA clone MGC:5814 IMAGE:3501141) mm
MU11KSUBA:AA277363_AT Phosphatidylserine synthase 1, mRNA (cDNA clone MGC:5814 IMAGE:3501141) mm
MU19KSUBC:TC41376_AT Phosphatidylserine synthase 1, mRNA (cDNA clone MGC:5814 IMAGE:3501141) mm
RG-U34C:RC_AI177921_AT phosphatidylserine synthase 1 (predicted) rn
RAE230A:1367497_AT phosphatidylserine synthase 1 (predicted) rn
RAE230B:1367497_AT phosphatidylserine synthase 1 (predicted) rn
RAT230_2:1367497_AT phosphatidylserine synthase 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6659 phosphatidylserine biosynthesis inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87373 Hypothetical protein ZC506.3 [Caenorhabditis elegans] ref|NP_509673.1| ZC506.3 [Caenorhabditis elegans] 0.0
blast CAE58758 Hypothetical protein CBG01950 [Caenorhabditis briggsae] 0.0
blast CAE69270 Hypothetical protein CBG15323 [Caenorhabditis briggsae] 1.0E-149
Protein Domains
Database ID Description E-Value
Pfam IPR004277 EMBL-EBI Phosphatidyl serine synthase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509673.1 9 93-112,125-147,151-173,233-255,270-292,341-358,371-393,406-428,433-455
ZC506.3.3 9 93-112,125-147,151-173,233-255,270-292,341-358,371-393,406-428,433-455
ZC506.3.2 9 93-112,125-147,151-173,233-255,270-292,341-358,371-393,406-428,433-455
ZC506.3.1 9 93-112,125-147,151-173,233-255,270-292,341-358,371-393,406-428,433-455
Sequence
Target Sequence 
>C. ELEGANS:174330_AT
aagtgtcaaatgtttgcgctttcacgctttccttctctcaatttatatgtttcatacgtt
ctcaaatttcaggtctagacatttttctaatgttttcttacttattttatatttttattg
cccatttctcggacatatcttatatatgattccatgtttcttttaatttggaaaatgttt
ttaggagatttataataataatctgcaggaatgcaattcgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTGTCAAATGTTTGCGCTTTCAC 207 159 16 Antisense
AAATGTTTGCGCTTTCACGCTTTCC 243 121 23 Antisense
TATATGTTTCATACGTTCTCAAATT 441 661 59 Antisense
ATACGTTCTCAAATTTCAGGTCTAG 670 23 69 Antisense
AAATTTCAGGTCTAGACATTTTTCT 122 117 79 Antisense
TCTAATGTTTTCTTACTTATTTTAT 576 613 101 Antisense
TTATATTTTTATTGCCCATTTCTCG 29 679 122 Antisense
TTTTTATTGCCCATTTCTCGGACAT 32 675 127 Antisense
GCCCATTTCTCGGACATATCTTATA 119 287 135 Antisense
ATCTTATATATGATTCCATGTTTCT 566 31 152 Antisense
TAATCTGCAGGAATGCAATTCGAAA 184 577 214 Antisense
Sequence Source Affymetrix Proprietary Database