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[174314_at]

Description C54160_rc /REP_DB=TREMBL Accession /GB=C54160 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk352d9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 33      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 42      6grp_total > 21      4grp_total > 58      6grp_highest_sub > 02      6grp_cgc > 62      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 790
1 2 3 4 range
A 826.1
(1.2)
P 		835.8
(1.3)
P 		981.5
(3)
P 		466.7
(-3)
P 		515
B 616.2
(-3)
P 		1257.1
(3)
P 		985.2
(0.5)
P 		734.6
(-1.9)
P 		641
C 794
(-0.8)
P 		1239.5
(3)
P 		977.2
(0.8)
P 		533.6
(-3)
P 		706
avr 745.4
(-1.1) 		1110.8
(3) 		981.3
(1.5) 		578.3
(-3) 		533

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 619
1 2 3 4 5 6 range
A 1000
(1.7)
P 		1105.2
(3)
P 		878.6
(0.1)
P 		629.1
(-3)
P 		799.3
(-0.9)
P 		666.3
(-2.5)
P 		476
B 919.1
(0.7)
P 		1064.3
(3)
P 		827.3
(-0.8)
P 		918.6
(0.7)
P 		842.7
(-0.6)
P 		691.5
(-3)
P 		373
C 700.2
(-1.4)
P 		1149.5
(3)
P 		1108.8
(2.6)
P 		819.6
(-0.2)
P 		601.1
(-2.3)
P 		530.5
(-3)
P 		619
avr 873.1
(0.1) 		1106.3
(3) 		938.2
(0.9) 		789.1
(-1) 		747.7
(-1.5) 		629.4
(-3) 		477
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 288
1 2 3 4 range
A 300.8
(3)
P 		181.4
(-0.1)
P 		256.6
(1.9)
P 		68.3
(-3)
P 		233
B 196.5
(-1.4)
P 		310.5
(3)
P 		228.3
(-0.2)
P 		156.5
(-3)
P 		154
C 262.9
(0.9)
P 		356.4
(3)
P 		340.6
(2.6)
P 		90
(-3)
P 		266
avr 253.4
(2) 		282.8
(3) 		275.2
(2.7) 		104.9
(-3) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 184.3
(-2.4)
P 		393.1
(3)
P 		191.3
(-2.2)
P 		159.6
(-3)
P 		241.2
(-0.9)
P 		162.2
(-2.9)
P 		234
B 319.8
(1.7)
P 		354.3
(3)
P 		200.9
(-3)
P 		243.3
(-1.3)
P 		342.4
(2.5)
P 		257.9
(-0.8)
P 		153
C 259.6
(0.1)
P 		350.8
(3)
P 		282.2
(0.8)
P 		164.8
(-3)
P 		194.5
(-2)
P 		177.6
(-2.6)
P 		186
avr 254.6
(-0.8) 		366.1
(3) 		224.8
(-1.8) 		189.2
(-3) 		259.4
(-0.6) 		199.2
(-2.7) 		177
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174314_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22202
Sequence Type Exemplar sequence
Representative Public ID C54160 NCBI
Target Description C54160_rc /REP_DB=TREMBL Accession /GB=C54160 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk352d9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076764(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076764 NCBI Caenorhabditis elegans UNCoordinated family member (unc-6) (unc-6) mRNA, complete cds. 11/11 None
F41C6.1.1 ENSEMBL cdna:known chromosome:CEL140:X:6889641:6897088:1 gene:F41C6.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F41C6.1.2 7/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6896743-6897033(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol unc-6
Ensembl F41C6.1
Entrez Gene 180961 Entrez gene
SwissProt P34710 EMBL-EBI
WormBase CE04538 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.26630.1.S1_S_AT similar to Netrin-1 precursor cfa
CANINE_2:CFAAFFX.26634.1.S1_AT similar to Netrin-1 precursor cfa
DROSOPHILA_2:1624408_AT Netrin-A dm
DROSGENOME1:143841_AT Netrin-A dm
DROSGENOME1:142249_AT Netrin-A dm
HG-U133_PLUS_2:208005_AT netrin 1 hs
HG-U133A:208005_AT netrin 1 hs
HG-FOCUS:208005_AT netrin 1 hs
HG-U133A_2:208005_AT netrin 1 hs
U133_X3P:G4758839_3P_AT netrin 1 hs
HG-U95C:51296_AT Netrin 1 hs
HG-U95D:77278_AT Netrin 1 hs
HG-U95E:80152_AT Netrin 1 hs
MOUSE430A_2:1422987_AT netrin 1 mm
MOUSE430_2:1422987_AT netrin 1 mm
MOE430A:1422987_AT netrin 1 mm
MU11KSUBA:AA270913_S_AT netrin 1 mm
MU11KSUBB:MSA.3316.0_S_AT netrin 1 mm
MG-U74AV2:97977_AT netrin 1 mm
MG-U74AV2:97728_AT netrin 1 mm
MOE430A:1454974_AT netrin 1 mm
MOUSE430A_2:1454974_AT netrin 1 mm
MOUSE430_2:1454974_AT netrin 1 mm
MU19KSUBC:TC40217_AT Netrin 1 (Ntn1), mRNA mm
RAE230A:1398269_AT netrin 1 rn
RAT230_2:1398269_AT netrin 1 rn
RAE230A:1368880_AT netrin 1 rn
RAT230_2:1368880_AT netrin 1 rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5578 extracellular matrix (sensu Metazoa) inferred from electronic annotation QuickGO AmiGO
5737 cytoplasm inferred from direct assay QuickGO AmiGO
30424 axon inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5102 receptor binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA80449 Uncoordinated protein 6 [Caenorhabditis elegans] ref|NP_509165.1| UNCoordinated family member (unc-6) [Caenorhabditis elegans] sp|P34710|UNC6_CAEEL Netrin unc-6 precursor (Uncoordinated protein 6) gb|AAA28157.1| unc-6 0.0
blast CAE65814 Hypothetical protein CBG10925 [Caenorhabditis briggsae] 0.0
blast NP_571104 netrin 1a [Danio rerio] gb|AAC60252.1| netrin-1a [Danio rerio] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008211 EMBL-EBI Laminin, N-terminal 2.7E-51
Pfam IPR001134 EMBL-EBI Netrin, C-terminal 5.1E-39
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 4.4E-19
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 1.2E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509165.1 1 5-24
F41C6.1.1 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:174314_AT
acatagacactcattcctactgggtactgtagcagtacctctgattttgctttaaatatg
attccataataacgcatgacgtttcacctctgtggtgcactattctctgttaacaccttt
tatctttttttttcaaacttctctcatgttgtggttgtgaatttcagtttctaccgttcc
attttctatgaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATAGACACTCATTCCTACTGGGT 388 105 32 Antisense
ACACTCATTCCTACTGGGTACTGTA 167 111 38 Antisense
ACTGTAGCAGTACCTCTGATTTTGC 580 97 57 Antisense
ATAACGCATGACGTTTCACCTCTGT 103 25 100 Antisense
TGACGTTTCACCTCTGTGGTGCACT 170 569 108 Antisense
CTGTGGTGCACTATTCTCTGTTAAC 582 237 121 Antisense
TCTCTGTTAACACCTTTTATCTTTT 621 609 135 Antisense
TTTTTTTCAAACTTCTCTCATGTTG 533 675 158 Antisense
AACTTCTCTCATGTTGTGGTTGTGA 88 139 167 Antisense
GTTGTGAATTTCAGTTTCTACCGTT 445 435 185 Antisense
TTCTACCGTTCCATTTTCTATGACA 496 691 200 Antisense
Sequence Source Affymetrix Proprietary Database