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[174312_at]

Description C59766_rc /REP_DB=TREMBL Accession /GB=C59766 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk442d5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 74      6grp_total > 95      4grp_total > 58      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 979.8
(1)
P 		881.6
(-1)
P 		1080.6
(3)
P 		781.5
(-3)
P 		299
B 904.5
(-1.7)
P 		959.2
(-0.2)
P 		1074.9
(3)
P 		859.5
(-3)
P 		215
C 877.7
(-1.7)
P 		1103.2
(3)
P 		979.1
(0.4)
P 		814.8
(-3)
P 		288
avr 920.7
(-0.3) 		981.3
(1.3) 		1044.9
(3) 		818.6
(-3) 		226

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 507
1 2 3 4 5 6 range
A 603.9
(-1.9)
P 		781.7
(1.1)
P 		534
(-3)
P 		683.6
(-0.5)
P 		891
(2.9)
P 		898.9
(3)
P 		365
B 503.8
(-2.1)
P 		567.7
(-1.2)
P 		440.2
(-3)
P 		717.5
(0.9)
P 		865.1
(3)
P 		757.8
(1.5)
P 		425
C 817.6
(1.4)
P 		458.3
(-3)
P 		783.8
(1)
P 		757.5
(0.7)
P 		947.3
(3)
P 		754.3
(0.6)
P 		489
avr 641.8
(-1.9) 		602.6
(-2.7) 		586
(-3) 		719.5
(-0.5) 		901.1
(3) 		803.7
(1.1) 		315
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 242
1 2 3 4 range
A 356.8
(3)
P 		191.4
(-1.1)
P 		282.5
(1.2)
P 		114.4
(-3)
P 		242
B 288.4
(3)
P 		236.9
(0.1)
P 		249.1
(0.8)
P 		183.2
(-3)
P 		105
C 290.6
(1.5)
P 		317.2
(2.3)
P 		341.2
(3)
P 		137.4
(-3)
P 		204
avr 311.9
(3) 		248.5
(0.7) 		290.9
(2.2) 		145
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 111.3
(-3)
P 		278
(3)
P 		116.3
(-2.8)
P 		173.4
(-0.8)
P 		268.9
(2.7)
P 		218.9
(0.9)
P 		167
B 175.3
(-1.3)
P 		189
(-1)
P 		106.9
(-3)
P 		190
(-1)
P 		351.5
(3)
P 		282.6
(1.3)
P 		245
C 303
(2.9)
P 		139.8
(-3)
P 		199.5
(-0.9)
P 		152.3
(-2.6)
P 		306.5
(3)
P 		252.5
(1.1)
P 		167
avr 196.5
(-1) 		202.3
(-0.8) 		140.9
(-3) 		171.9
(-1.9) 		309
(3) 		251.3
(0.9) 		168
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174312_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20779
Sequence Type Exemplar sequence
Representative Public ID C59766 NCBI
Target Description C59766_rc /REP_DB=TREMBL Accession /GB=C59766 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk442d5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027249(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027249 NCBI Caenorhabditis elegans Y25C1A.7c (Y25C1A.7) mRNA, complete cds. 11/11 None
Y25C1A.7a.1 ENSEMBL cdna:known chromosome:CEL140:II:3062544:3071057:-1 gene:Y25C1A.7 9/11 None
Y25C1A.7a.2 ENSEMBL cdna:known chromosome:CEL140:II:3062544:3071059:-1 gene:Y25C1A.7 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000041893 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173654 3 1 NM_001027249 174312_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3062557-3062901(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y25C1A.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022420 Y25C1A.7c [Caenorhabditis elegans] gb|AAX88818.1| Hypothetical protein Y25C1A.7c [Caenorhabditis elegans] 0.0
blast T33904 hypothetical protein Y25C1A.7a - Caenorhabditis elegans 0.0
blast NP_494438 Y25C1A.7a [Caenorhabditis elegans] gb|AAX88817.1| Hypothetical protein Y25C1A.7a [Caenorhabditis elegans] 1.0E-154
blast NP_494439 Y25C1A.7b [Caenorhabditis elegans] gb|AAD12839.1| Hypothetical protein Y25C1A.7b [Caenorhabditis elegans] 1.0E-150
blast NP_494438 Y25C1A.7a [Caenorhabditis elegans] gb|AAX88817.1| Hypothetical protein Y25C1A.7a [Caenorhabditis elegans] 1.0E-79
blast NP_001022420 Y25C1A.7c [Caenorhabditis elegans] gb|AAX88818.1| Hypothetical protein Y25C1A.7c [Caenorhabditis elegans] 1.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 1.7E-72
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 6.2E-31
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 2.1E-32
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022420.1 5 108-130,145-167,180-202,206-228,241-263
Y25C1A.7a.1 5 107-129,232-254,264-286,291-313,323-345
Y25C1A.7a.2 5 107-129,232-254,264-286,291-313,323-345
Sequence
Target Sequence 
>C. ELEGANS:174312_AT
aagcaccgaaaagttgccggccgctgaaatttctgaaaattcgactgaagccgccgccgc
cgcaacgaattccactgaaaaaaatcaataaattatcgatttttctgcaaaaaaaaaagt
tttttttctcgtttttgccctgctttacccccattttacccacactaaaatcgcataatt
ccggaaaattttatctacgtcacccaacctgttccacttgtagtcacttactgtaccttt
tcttgtgatattatgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCACCGAAAAGTTGCCGGCCGCT 180 151 65 Antisense
CGAAAAGTTGCCGGCCGCTGAAATT 259 251 71 Antisense
CGCCGCAACGAATTCCACTGAAAAA 131 257 122 Antisense
CCATTTTACCCACACTAAAATCGCA 455 243 215 Antisense
CCCACACTAAAATCGCATAATTCCG 76 257 223 Antisense
CTAAAATCGCATAATTCCGGAAAAT 27 225 229 Antisense
CTGTTCCACTTGTAGTCACTTACTG 708 235 273 Antisense
TCCACTTGTAGTCACTTACTGTACC 443 587 277 Antisense
GTAGTCACTTACTGTACCTTTTCTT 239 455 284 Antisense
ACTTACTGTACCTTTTCTTGTGATA 281 103 290 Antisense
GTACCTTTTCTTGTGATATTATGTG 41 459 297 Antisense
Sequence Source Affymetrix Proprietary Database