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[174189_at]

Description CEC578_rc /REP_DB=TREMBL Accession /GB=C07578 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk142f4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 17      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 50      6grp_total > 17      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 376
1 2 3 4 range
A 753.4
(3)
P 		453.6
(-2.1)
P 		416.3
(-2.7)
P 		398.3
(-3)
P 		355
B 677.1
(3)
P 		515.9
(-0.2)
P 		377.7
(-3)
P 		457.1
(-1.4)
P 		299
C 677.6
(3)
P 		613
(1.4)
P 		429.7
(-3)
P 		443.8
(-2.7)
P 		248
avr 702.7
(3) 		527.5
(-0.6) 		407.9
(-3) 		433.1
(-2.5) 		295

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 712
1 2 3 4 5 6 range
A 737
(3)
P 		553.1
(0.7)
P 		269
(-2.9)
P 		263.5
(-3)
P 		326.6
(-2.2)
P 		320
(-2.3)
P 		474
B 907.2
(3)
P 		554.1
(-0.1)
P 		304.2
(-2.3)
P 		379.2
(-1.7)
P 		228.6
(-3)
P 		338.4
(-2)
P 		679
C 941
(3)
P 		462.7
(-1.3)
P 		390.3
(-1.9)
P 		272.1
(-3)
P 		338.5
(-2.4)
P 		395.9
(-1.9)
P 		669
avr 861.7
(3) 		523.3
(-0.6) 		321.2
(-2.8) 		304.9
(-2.9) 		297.9
(-3) 		351.4
(-2.4) 		564
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 216
1 2 3 4 range
A 274.3
(3)
P 		98.5
(-1.9)
P 		108.8
(-1.6)
P 		58.3
(-3)
P 		216
B 215.9
(3)
P 		127.4
(-1.1)
P 		87.5
(-3)
P 		97.4
(-2.5)
P 		128
C 224.3
(3)
P 		176.2
(1.1)
P 		149.7
(0)
P 		74.8
(-3)
P 		150
avr 238.2
(3) 		134
(-0.9) 		115.3
(-1.6) 		76.8
(-3) 		161

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 294
1 2 3 4 5 6 range
A 135.9
(0.4)
P 		196.7
(3)
P 		58.6
(-3)
P 		66.8
(-2.6)
P 		98.6
(-1.3)
P 		77.9
(-2.2)
P 		138
B 315.7
(3)
P 		184.4
(-0.3)
P 		73.9
(-3)
P 		100.4
(-2.3)
P 		92.9
(-2.5)
P 		126.2
(-1.7)
P 		242
C 348.8
(3)
P 		141.2
(-1.2)
P 		99.3
(-2.1)
P 		54.7
(-3)
P 		109.5
(-1.9)
P 		132.5
(-1.4)
P 		294
avr 266.8
(3) 		174.1
(0.1) 		77.3
(-2.9) 		74
(-3) 		100.3
(-2.2) 		112.2
(-1.8) 		193
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174189_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22366
Sequence Type Exemplar sequence
Representative Public ID CEC578 NCBI
Target Description CEC578_rc /REP_DB=TREMBL Accession /GB=C07578 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk142f4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077921(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077921 NCBI Caenorhabditis elegans Argonaute (plant)-Like Gene family member (alg-1) (alg-1) mRNA, complete cds. 11/11 None
F48F7.1 ENSEMBL cdna:known chromosome:CEL140:X:13951203:13956174:1 gene:F48F7.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13954698-13954967(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol alg-1
Ensembl F48F7.1
Entrez Gene 181504 Entrez gene
SwissProt Q20578 EMBL-EBI
WormBase CE31525 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:19521_S_AT argonaute protein, putative / AGO, putative at
ATH1-121501:264066_AT argonaute protein, putative / AGO, putative at
CANINE:1585521_AT similar to eukaryotic translation initiation factor 2C, 2 cfa
CANINE_2:CFA.16380.2.A1_AT similar to eukaryotic translation initiation factor 2C, 2 cfa
CANINE_2:CFA.8562.1.A1_S_AT similar to eukaryotic translation initiation factor 2C, 2 cfa
DROSOPHILA_2:1632602_S_AT Argonaute 1 dm
DROSGENOME1:154777_AT Argonaute 1 dm
CHICKEN:GGAAFFX.10340.4.S1_S_AT similar to eukaryotic translation initiation factor 2C, 2; argonaute 2 gga
HU35KSUBC:RC_AA406534_AT eukaryotic translation initiation factor 2C, 2 hs
HU35KSUBD:RC_R30931_AT eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS.193053.0.A1_3P_AT eukaryotic translation initiation factor 2C, 2 hs
HG-U133_PLUS_2:225827_AT eukaryotic translation initiation factor 2C, 2 hs
HG-U133B:225827_AT eukaryotic translation initiation factor 2C, 2 hs
HG-U95B:56226_AT eukaryotic translation initiation factor 2C, 2 hs
HG-U95E:74599_AT eukaryotic translation initiation factor 2C, 2 hs
HG-U133_PLUS_2:213310_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133A_2:213310_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133A:213310_AT Eukaryotic translation initiation factor 2C, 2 hs
HU35KSUBA:C01527_AT Eukaryotic translation initiation factor 2C, 2 hs
HU35KSUBB:RC_T03861_AT Eukaryotic translation initiation factor 2C, 2 hs
HU35KSUBB:RC_R24059_AT Eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS2.406935.1.S1_3P_AT Eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS.324504.1.A1_3P_AT Eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS.287734.0.S1_3P_AT Eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS.193053.1.A1_3P_AT Eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS.165337.1.A1_3P_X_AT Eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS.165337.1.A1_3P_AT Eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS.165337.0.A1_3P_A_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133_PLUS_2:1562865_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133A_2:222294_S_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133_PLUS_2:222294_S_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133A:222294_S_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133_PLUS_2:225569_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133B:225569_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133_PLUS_2:233244_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133B:233244_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133_PLUS_2:235766_X_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133B:235766_X_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U95AV2:38505_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U95C:48698_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U95C:55539_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U95B:58633_S_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U95C:65694_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133_PLUS_2:229841_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U133B:229841_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U95C:50191_I_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U95C:50196_F_AT Eukaryotic translation initiation factor 2C, 2 hs
HG-U95B:51520_AT Eukaryotic translation initiation factor 2C, 2 hs
HU35KSUBA:W73104_AT Eukaryotic translation initiation factor 2C, 2 hs
U133_X3P:HS.324504.0.A1_3P_AT Eukaryotic translation initiation factor 2C, 2 hs
HU35KSUBB:RC_N59129_AT Eukaryotic translation initiation factor 2C, 2 hs
MU11KSUBA:AA104073_S_AT eukaryotic translation initiation factor 2C, 2 mm
MG-U74AV2:103994_AT eukaryotic translation initiation factor 2C, 2 mm
MOE430A:1426366_AT eukaryotic translation initiation factor 2C, 2 mm
MOUSE430A_2:1426366_AT eukaryotic translation initiation factor 2C, 2 mm
MOUSE430_2:1426366_AT eukaryotic translation initiation factor 2C, 2 mm
MG-U74BV2:114538_AT Eukaryotic translation initiation factor 2C, 2 (Eif2c2), mRNA mm
MG-U74CV2:135956_AT Eukaryotic translation initiation factor 2C, 2 (Eif2c2), mRNA mm
MU19KSUBA:TC19897_I_AT Eukaryotic translation initiation factor 2C, 2 (Eif2c2), mRNA mm
MU19KSUBB:TC29047_AT Eukaryotic translation initiation factor 2C, 2 (Eif2c2), mRNA mm
RAE230A:1368866_AT GERp95 rn
RAT230_2:1368866_AT GERp95 rn
RAE230A:1368867_AT GERp95 rn
RAT230_2:1368867_AT GERp95 rn
RG-U34A:RC_H31692_AT GERp95 rn
Protein Similarities
Method ID Description E-Value
blast CAA93512 Hypothetical protein F48F7.1 [Caenorhabditis elegans] emb|CAA93496.2| Hypothetical protein F48F7.1 [Caenorhabditis elegans] ref|NP_510322.2| Argonaute (plant)-Like Gene family member (alg-1) [Caenorhabditis elegans] 0.0
blast ABA18180 argonaute-like [Caenorhabditis elegans] 0.0
blast CAE63062 Hypothetical protein CBG07340 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 1.0E-57
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174189_AT
ttgaatattgtcatgtctacctaatatctatcttgataattctgtgtctttatctgtatc
tgtatctccattcaaattgatttcaatatgctacaatgttataatgtttttctataatcg
gactcttatgttttcaaattccatatttaatttgtaattctaattgccgctacctctcgt
gtaactgttttctttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAATATTGTCATGTCTACCTAAT 195 705 15 Antisense
GTCATGTCTACCTAATATCTATCTT 290 467 24 Antisense
ATCTATCTTGATAATTCTGTGTCTT 87 33 40 Antisense
AATTCTGTGTCTTTATCTGTATCTG 538 177 52 Antisense
TTATCTGTATCTGTATCTCCATTCA 267 681 64 Antisense
GTATCTGTATCTCCATTCAAATTGA 512 453 70 Antisense
AATGTTTTTCTATAATCGGACTCTT 594 71 117 Antisense
TATAATCGGACTCTTATGTTTTCAA 669 659 127 Antisense
TTGTAATTCTAATTGCCGCTACCTC 553 705 166 Antisense
CTACCTCTCGTGTAACTGTTTTCTT 372 225 184 Antisense
CCTCTCGTGTAACTGTTTTCTTTCT 663 267 187 Antisense
Sequence Source Affymetrix Proprietary Database