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[174185_at]

Description C53232_rc /REP_DB=TREMBL Accession /GB=C53232 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk295e3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 48      4grp_highest_8_8 > 42      4grp_high_8_8 > 49      6grp_highest_nonorm > 78      6grp_total > 59      4grp_total > 54      6grp_highest_sub > Cu      6grp_cgc > 07      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 851
1 2 3 4 range
A 1581
(3)
P 		1110.4
(-0.9)
P 		1050.1
(-1.4)
P 		864.4
(-3)
P 		717
B 1625.2
(3)
P 		1200.7
(-1)
P 		1383.8
(0.7)
P 		990.1
(-3)
P 		635
C 1715.8
(3)
P 		1428.1
(-0.7)
P 		1439.8
(-0.5)
P 		1248.9
(-3)
P 		467
avr 1640.7
(3) 		1246.4
(-0.9) 		1291.2
(-0.5) 		1034.5
(-3) 		606

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1435
1 2 3 4 5 6 range
A 2091.9
(3)
P 		1381.5
(-2.6)
P 		1326.8
(-3)
P 		1471.7
(-1.9)
P 		1563.3
(-1.1)
P 		1739.8
(0.2)
P 		765
B 1805.7
(3)
P 		802.1
(-3)
P 		1001.1
(-1.8)
P 		1602.3
(1.8)
P 		1134.7
(-1)
P 		1474.9
(1)
P 		1004
C 1614.8
(0.2)
P 		949.5
(-2.9)
P 		1679.5
(0.5)
P 		918.2
(-3)
P 		1771.7
(0.9)
P 		2237.3
(3)
P 		1319
avr 1837.5
(3) 		1044.4
(-3) 		1335.8
(-0.8) 		1330.7
(-0.8) 		1489.9
(0.4) 		1817.3
(2.8) 		793
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 449
1 2 3 4 range
A 575.6
(3)
P 		241
(-1.5)
P 		274.5
(-1)
P 		126.5
(-3)
P 		449
B 518.3
(3)
P 		296.6
(-1.3)
P 		320.7
(-0.9)
P 		211
(-3)
P 		307
C 568
(3)
P 		410.6
(0.4)
P 		501.8
(1.9)
P 		210.6
(-3)
P 		357
avr 554
(3) 		316.1
(-0.8) 		365.7
(-0) 		182.7
(-3) 		371

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 564
1 2 3 4 5 6 range
A 385.6
(-0.1)
P 		491.3
(3)
P 		289
(-3)
P 		373.2
(-0.5)
P 		471.7
(2.4)
P 		423.6
(1)
P 		202
B 628.3
(3)
P 		267
(-2.6)
P 		243.1
(-3)
P 		424.4
(-0.2)
P 		461
(0.4)
P 		550
(1.8)
P 		385
C 598.5
(1.4)
P 		289.7
(-1.9)
P 		427.5
(-0.4)
P 		184.6
(-3)
P 		573.3
(1.1)
P 		749
(3)
P 		564
avr 537.5
(2.1) 		349.3
(-2.3) 		319.9
(-3) 		327.4
(-2.8) 		502
(1.3) 		574.2
(3) 		254
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174185_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20264
Sequence Type Exemplar sequence
Representative Public ID C53232 NCBI
Target Description C53232_rc /REP_DB=TREMBL Accession /GB=C53232 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk295e3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059185(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059185 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-4) (frm-4) mRNA, complete cds. 11/11 None
C24A11.8a ENSEMBL cdna:known chromosome:CEL140:I:5402898:5408347:-1 gene:C24A11.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172189 2 1 NM_059185 174185_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5402962-5403250(-) 100.0 100.0
Public Domain and Genome References
Ensembl C24A11.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD32269 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 4, isoform a [Caenorhabditis elegans] ref|NP_491586.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-4) [Caenorhabditis elegans] 0.0
blast AAK68201 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 4, isoform b [Caenorhabditis elegans] ref|NP_491587.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-4) [Caenorhabditis elegans] 0.0
blast CAE66927 Hypothetical protein CBG12316 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000299 EMBL-EBI Band 4.1 2.6E-41
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491586.1 1 568-590
C24A11.8a 1 568-590
Sequence
Target Sequence 
>C. ELEGANS:174185_AT
ttcttttatatctaatttcccaaatgtattttacaatttcttttctttttcattttaagt
ttaatatgtaacttttcgtctaattaaataattcgtaacagttatacaaattcatcctct
tcgctggttttttcttcttttttaaaattccttttctttttctcgctcacttgctttatt
ttttctcccagttataccgatgtacccaataatcaaccaccgcctaatgacacaaaattc
caa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTTTTATATCTAATTTCCCAAAT 622 689 18 Antisense
TAATTTCCCAAATGTATTTTACAAT 226 607 30 Antisense
TTAATATGTAACTTTTCGTCTAATT 621 683 78 Antisense
AACAGTTATACAAATTCATCCTCTT 447 137 114 Antisense
TTCTCGCTCACTTGCTTTATTTTTT 457 693 177 Antisense
TATTTTTTCTCCCAGTTATACCGAT 403 661 194 Antisense
TCTCCCAGTTATACCGATGTACCCA 467 609 201 Antisense
GATGTACCCAATAATCAACCACCGC 610 409 216 Antisense
AATAATCAACCACCGCCTAATGACA 668 151 225 Antisense
CAACCACCGCCTAATGACACAAAAT 382 187 231 Antisense
ACCGCCTAATGACACAAAATTCCAA 581 89 236 Antisense
Sequence Source Affymetrix Proprietary Database