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[174181_s_at] phenylalanyl-tRNA synthetase

Description C57416_rc /REP_DB=TREMBL Accession /GB=C57416 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk303c5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 37      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 67      6grp_total > 17      4grp_total > 08      6grp_highest_sub > Cu      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1904
1 2 3 4 range
A 2784.3
(-2.4)
P 		2654.2
(-3)
P 		3512.8
(0.6)
P 		4072.4
(3)
P 		1418
B 2808.6
(-3)
P 		2940.3
(-2.5)
P 		3425.9
(-0.9)
P 		4558.5
(3)
P 		1750
C 2850
(-3)
P 		3050.4
(-1.5)
P 		3672.6
(3)
P 		3644.6
(2.8)
P 		823
avr 2814.3
(-3) 		2881.6
(-2.7) 		3537.1
(0.4) 		4091.8
(3) 		1278

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 806
1 2 3 4 5 6 range
A 2026.1
(3)
P 		1715.2
(-0.2)
P 		1942.7
(2.1)
P 		1693.7
(-0.5)
P 		1451.9
(-3)
P 		1622.4
(-1.2)
P 		574
B 2257.4
(3)
P 		1933.9
(-0.1)
P 		1823.3
(-1.2)
P 		1837.1
(-1.1)
P 		1639.8
(-3)
P 		1831.5
(-1.1)
P 		618
C 1899.2
(1.2)
P 		1794.2
(-0.1)
P 		1812.8
(0.1)
P 		1570.9
(-3)
P 		1610.7
(-2.5)
P 		2034.4
(3)
P 		464
avr 2060.9
(3) 		1814.4
(0) 		1859.6
(0.6) 		1700.6
(-1.4) 		1567.5
(-3) 		1829.4
(0.2) 		493
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 704
1 2 3 4 range
A 1013.8
(3)
P 		576.1
(-3)
P 		918.3
(1.7)
P 		596.1
(-2.7)
P 		438
B 895.6
(1.1)
P 		726.2
(-3)
P 		793.9
(-1.3)
P 		971.4
(3)
P 		245
C 943.4
(-0)
P 		877
(-0.6)
P 		1280
(3)
P 		614.5
(-3)
P 		666
avr 950.9
(2) 		726.4
(-3) 		997.4
(3) 		727.3
(-3) 		271

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 470
1 2 3 4 5 6 range
A 373.5
(-3)
P 		610
(3)
P 		423.1
(-1.7)
P 		429.6
(-1.6)
P 		438.1
(-1.4)
P 		395
(-2.5)
P 		237
B 785.5
(3)
P 		643.7
(0.5)
P 		442.7
(-3)
P 		486.6
(-2.2)
P 		666.2
(0.9)
P 		683
(1.2)
P 		343
C 703.9
(3)
P 		547.5
(0.6)
P 		461.4
(-0.8)
P 		315.9
(-3)
P 		521.2
(0.2)
P 		681
(2.6)
P 		388
avr 621
(3) 		600.4
(2.4) 		442.4
(-2.1) 		410.7
(-3) 		541.8
(0.7) 		586.3
(2) 		210
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174181_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20600
Sequence Type Exemplar sequence
Representative Public ID C57416 NCBI
Target Description C57416_rc /REP_DB=TREMBL Accession /GB=C57416 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk303c5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063384(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063384 NCBI Caenorhabditis elegans phenylalanyl (F) tRNA Synthetase family member (frs-2) (frs-2) mRNA, complete cds. 11/11 A 
GENEFINDER00000010412 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8464774:8466870:1 11/11 None
F22B5.9 ENSEMBL cdna:known chromosome:CEL140:II:8464770:8466996:1 gene:F22B5.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000010407 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8466519-8466919(+) 100.0 100.0
Public Domain and Genome References
Gene Title phenylalanyl-tRNA synthetase
Gene Symbol frs-2
Ensembl F22B5.9
Entrez Gene 174351 Entrez gene
SwissProt Q19713 EMBL-EBI
WormBase CE20708 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:259920_AT tRNA synthetase beta subunit family protein at
CANINE:1585166_X_AT similar to Phenylalanyl-tRNA synthetase beta chain (Phenylalanine--tRNA ligase beta chain) (PheRS) cfa
CANINE:1585165_AT similar to Phenylalanyl-tRNA synthetase beta chain (Phenylalanine--tRNA ligase beta chain) (PheRS) cfa
CANINE_2:CFA.4911.1.A1_AT similar to Phenylalanyl-tRNA synthetase beta chain (Phenylalanine--tRNA ligase beta chain) (PheRS) cfa
CANINE_2:CFA.4911.1.A1_S_AT similar to Phenylalanyl-tRNA synthetase beta chain (Phenylalanine--tRNA ligase beta chain) (PheRS) cfa
CANINE_2:CFAAFFX.24751.1.S1_AT similar to Phenylalanyl-tRNA synthetase beta chain (Phenylalanine--tRNA ligase beta chain) (PheRS) cfa
DROSOPHILA_2:1638711_AT dm
DROSGENOME1:141570_AT dm
CHICKEN:GGA.6269.2.S1_S_AT phenylalanine-tRNA synthetase-like, beta subunit gga
CHICKEN:GGAAFFX.12676.1.S1_S_AT phenylalanine-tRNA synthetase-like, beta subunit gga
HG-U133_PLUS_2:232063_X_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HG-U133B:232063_X_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HG-U133_PLUS_2:223036_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HG-U133B:223036_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HG-U133_PLUS_2:223035_S_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HG-U133B:223035_S_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HU35KSUBA:R71427_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HU35KSUBB:RC_N29812_AT phenylalanine-tRNA synthetase-like, beta subunit hs
U133_X3P:G7768937_3P_X_AT phenylalanine-tRNA synthetase-like, beta subunit hs
U133_X3P:G7768937_3P_AT phenylalanine-tRNA synthetase-like, beta subunit hs
U133_X3P:G6841565_3P_A_AT phenylalanine-tRNA synthetase-like, beta subunit hs
U133_X3P:HS.118570.0.A1_3P_S_AT phenylalanine-tRNA synthetase-like, beta subunit hs
U133_X3P:232063_3P_X_AT phenylalanine-tRNA synthetase-like, beta subunit hs
U133_X3P:223036_3P_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HG-U95B:46688_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HG-U95E:81316_AT phenylalanine-tRNA synthetase-like, beta subunit hs
HG-U95C:61141_AT Phenylalanine-tRNA synthetase-like, beta subunit hs
MOUSE430_2:1430986_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOUSE430A_2:1430986_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOE430A:1430986_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MU11KSUBA:AA154688_S_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MU11KSUBB:MSA.7828.0_S_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MU11KSUBB:MSA.13639.0_S_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MG-U74AV2:94494_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MG-U74CV2:168671_I_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MG-U74CV2:168126_S_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MG-U74CV2:170954_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MG-U74CV2:169178_R_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOE430A:1423709_S_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOUSE430A_2:1423709_S_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOUSE430_2:1423709_S_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOE430A:1423708_A_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOUSE430A_2:1423708_A_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOUSE430_2:1423708_A_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOE430A:1435103_X_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOUSE430A_2:1435103_X_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MOUSE430_2:1435103_X_AT phenylalanine-tRNA synthetase-like, beta subunit mm
MU19KSUBB:TC29869_AT Phenylalanine-tRNA synthetase-like, beta subunit (Farslb), mRNA mm
RG-U34C:RC_AI178283_AT phenylalanine-tRNA synthetase-like, beta subunit rn
RAE230A:1371453_AT phenylalanine-tRNA synthetase-like, beta subunit rn
RAT230_2:1371453_AT phenylalanine-tRNA synthetase-like, beta subunit rn
RAT230_2:1397073_AT Phenylalanine-tRNA synthetase-like, beta subunit rn
RAE230B:1397073_AT Phenylalanine-tRNA synthetase-like, beta subunit rn
YEAST_2:1777242_AT Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate the active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6432 phenylalanyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4826 phenylalanine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90360 Hypothetical protein F22B5.9 [Caenorhabditis elegans] ref|NP_495785.1| phenylalanyl (F) tRNA Synthetase family member (frs-2) [Caenorhabditis elegans] sp|Q19713|SYFB_CAEEL Phenylalanyl-tRNA synthetase beta chain (Phenylalanine--tRNA ligase beta chain) (PheRS) 0.0
blast CAE57636 Hypothetical protein CBG00621 [Caenorhabditis briggsae] 0.0
blast AAH85625 Hypothetical protein LOC493608 [Danio rerio] ref|NP_001007769.1| hypothetical protein LOC493608 [Danio rerio] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005146 EMBL-EBI B3/4 1.1E-28
Pfam IPR005147 EMBL-EBI tRNA synthetase B5 domain 1.8E-29
Sequence
Target Sequence 
>C. ELEGANS:174181_S_AT
aatgctaaatgtcaatcctgcgcgcgacggaactggatactacatcgaagccgatgaaaa
ttcaacctacttcccaggacggtgtgccaagattatcggtccaaagggagtcgttctcgg
acatatcggcaagagctctccatccagaagttatcacctccttcgggctcacattgccat
gtggagctgtcgaaatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCTAAATGTCAATCCTGCGCGC 708 165 50 Antisense
CTGCGCGCGACGGAACTGGATACTA 675 231 67 Antisense
AACTGGATACTACATCGAAGCCGAT 562 141 80 Antisense
GATGAAAATTCAACCTACTTCCCAG 476 391 102 Antisense
TTCCCAGGACGGTGTGCCAAGATTA 547 693 120 Antisense
GGTGTGCCAAGATTATCGGTCCAAA 110 501 130 Antisense
TCGGTCCAAAGGGAGTCGTTCTCGG 544 545 145 Antisense
AAGGGAGTCGTTCTCGGACATATCG 602 95 153 Antisense
TCCAGAAGTTATCACCTCCTTCGGG 46 589 192 Antisense
TTCGGGCTCACATTGCCATGTGGAG 616 695 211 Antisense
TGCCATGTGGAGCTGTCGAAATCAA 406 585 224 Antisense
Sequence Source Affymetrix Proprietary Database