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[174179_s_at]

Description C32071_rc /REP_DB=TREMBL Accession /GB=C32071 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk319e7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 78      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 91      6grp_total > 69      4grp_total > 39      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 287.3
(3)
P 		178.3
(-2.4)
P 		166.7
(-3)
P 		170
(-2.8)
P 		121
B 232.7
(3)
P 		198.3
(0.7)
P 		144.5
(-3)
P 		219
(2.1)
P 		88
C 288.9
(3)
P 		156.8
(-3)
P 		194.7
(-1.3)
P 		229.3
(0.3)
P 		132
avr 269.6
(3) 		177.8
(-2.5) 		168.6
(-3) 		206.1
(-0.8) 		101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 199.7
(-1.6)
P 		191.4
(-2.2)
P 		241
(1.5)
P 		181.2
(-3)
P 		229.1
(0.6)
P 		261.5
(3)
P 		80
B 257
(0.1)
P 		160.8
(-3)
P 		177.5
(-2.5)
P 		199.6
(-1.8)
P 		210.1
(-1.4)
P 		348.7
(3)
P 		188
C 212.2
(-0.3)
P 		177.7
(-1.8)
P 		153.7
(-2.8)
P 		148.8
(-3)
P 		213.5
(-0.2)
P 		288.7
(3)
P 		140
avr 223
(-0.7) 		176.6
(-3) 		190.7
(-2.3) 		176.5
(-3) 		217.6
(-1) 		299.6
(3) 		123
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 104.6
(3)
P 		38.7
(-2)
P 		43.6
(-1.6)
P 		24.9
(-3)
P 		80
B 74.2
(3)
P 		49
(-0.7)
P 		33.5
(-3)
P 		46.7
(-1.1)
P 		41
C 95.6
(3)
P 		45.1
(-2.3)
P 		67.8
(0.1)
P 		38.7
(-3)
P 		57
avr 91.5
(3) 		44.3
(-2.2) 		48.3
(-1.7) 		36.8
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 36.8
(-3)
P 		68.1
(2.8)
P 		52.5
(-0.1)
P 		45.9
(-1.3)
P 		69.1
(3)
P 		63.7
(2)
P 		32
B 89.4
(0.2)
P 		53.5
(-2.3)
P 		43.1
(-3)
P 		52.9
(-2.3)
P 		85.4
(-0.1)
P 		130
(3)
P 		87
C 78.7
(1.4)
P 		54.2
(-0.8)
P 		39.1
(-2.2)
P 		29.9
(-3)
P 		69.1
(0.5)
P 		96.7
(3)
P 		67
avr 68.3
(-0.2) 		58.6
(-1.3) 		44.9
(-2.8) 		42.9
(-3) 		74.5
(0.5) 		96.8
(3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174179_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20403
Sequence Type Exemplar sequence
Representative Public ID C32071 NCBI
Target Description C32071_rc /REP_DB=TREMBL Accession /GB=C32071 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk319e7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170949(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170949 NCBI Caenorhabditis elegans Y105E8A.17 (Y105E8A.17) mRNA, complete cds. 11/11 None
SNAP00000013340 ENSEMBL cdna:SNAP chromosome:CEL140:I:14470285:14502472:1 9/11 None
GENEFINDER00000013495 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14477624:14484603:1 11/11 None
Y105E8A.17 ENSEMBL cdna:known chromosome:CEL140:I:14477624:14484839:1 gene:Y105E8A.17 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14482892-14484760(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ekl-4
Ensembl Y105E8A.17
Entrez Gene 173310 Entrez gene
SwissProt Q8WQA7 EMBL-EBI
WormBase CE29836 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16245_AT myb family transcription factor at
CANINE:1603082_AT similar to DNMT1 associated protein-1 cfa
CANINE_2:CFA.20645.1.S1_S_AT similar to DNMT1 associated protein-1 cfa
DROSOPHILA_2:1638500_AT dm
DROSGENOME1:154715_AT dm
CHICKEN:GGAAFFX.6347.1.S1_AT similar to DNMT1 associated protein-1 gga
HU35KSUBB:RC_AA034314_AT DNA methyltransferase 1 associated protein 1 hs
HU35KSUBB:RC_F08876_AT DNA methyltransferase 1 associated protein 1 hs
HG-U133B:224163_S_AT DNA methyltransferase 1 associated protein 1 hs
HG-U133_PLUS_2:224163_S_AT DNA methyltransferase 1 associated protein 1 hs
U133_X3P:G12052837_3P_A_AT DNA methyltransferase 1 associated protein 1 hs
HG-U95C:65954_AT DNA methyltransferase 1 associated protein 1 hs
MU11KSUBA:C80866_RC_G_AT DNA methyltransferase 1-associated protein 1 mm
MU11KSUBA:C80866_RC_AT DNA methyltransferase 1-associated protein 1 mm
MG-U74AV2:96793_AT DNA methyltransferase 1-associated protein 1 mm
MG-U74BV2:112197_AT DNA methyltransferase 1-associated protein 1 mm
MOE430A:1417742_A_AT DNA methyltransferase 1-associated protein 1 mm
MOUSE430A_2:1417742_A_AT DNA methyltransferase 1-associated protein 1 mm
MOUSE430_2:1417742_A_AT DNA methyltransferase 1-associated protein 1 mm
MU19KSUBA:TC15075_AT DNA methyltransferase 1-associated protein 1 (Dmap1), mRNA mm
RG-U34B:RC_AA924980_AT similar to DNMT1 associated protein-1 rn
RAE230A:1389764_AT similar to DNMT1 associated protein-1 rn
RAT230_2:1389764_AT similar to DNMT1 associated protein-1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16481 negative regulation of transcription inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAD21666 Hypothetical protein Y105E8A.17 [Caenorhabditis elegans] ref|NP_740945.1| Y105E8A.17 [Caenorhabditis elegans] 0.0
blast CAE64139 Hypothetical protein CBG08755 [Caenorhabditis briggsae] 1.0E-171
blast CAE64139 Hypothetical protein CBG08755 [Caenorhabditis briggsae] 1.0E-169
blast XP_392117 PREDICTED: similar to ENSANGP00000017895 [Apis mellifera] 1.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR008468 EMBL-EBI DNA methyltransferase 1-associated 1 1.0E-126
Pfam IPR008468 EMBL-EBI DNA methyltransferase 1-associated 1 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174179_S_AT
tggtggaaaaggacaaccggcaactaatcgacgaattacttcatatattgatgctagttc
taaggatcttactgcgatagcgtcccgaaagcgaaaaacaattgcaacaactccaacaat
tactactactacatcatcatcatttgtctcctcatcgaattctccatcaacatcagctgc
tagtgatccgaaaagaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGTGGAAAAGGACAACCGGCAACT 308 549 25 Antisense
GGACAACCGGCAACTAATCGACGAA 208 527 35 Antisense
GCAACTAATCGACGAATTACTTCAT 313 319 44 Antisense
TACTTCATATATTGATGCTAGTTCT 224 645 61 Antisense
TGCTAGTTCTAAGGATCTTACTGCG 171 581 76 Antisense
AAAACAATTGCAACAACTCCAACAA 330 129 119 Antisense
ATTACTACTACTACATCATCATCAT 681 11 143 Antisense
CATCGAATTCTCCATCAACATCAGC 279 211 177 Antisense
TCGAATTCTCCATCAACATCAGCTG 104 601 179 Antisense
TCAACATCAGCTGCTAGTGATCCGA 223 631 191 Antisense
CAGCTGCTAGTGATCCGAAAAGAAT 637 201 198 Antisense
Sequence Source Affymetrix Proprietary Database