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[174167_at]

Description C31686_rc /REP_DB=TREMBL Accession /GB=C31686 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk290e1 : 3prime end, single read.
Cluster 4grp_high_8_8 > 26      6grp_total > 16      4grp_total > 38      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 244
1 2 3 4 range
A 332.9
(3)
P 		185.1
(-0.9)
P 		103.9
(-3)
P 		209.1
(-0.2)
P 		229
B 348.3
(3)
P 		234.1
(0.1)
P 		115.3
(-3)
P 		239.7
(0.2)
P 		233
C 347.3
(3)
P 		154.9
(-2.5)
P 		138.1
(-3)
M 		212.2
(-0.9)
P 		209
avr 342.8
(3) 		191.4
(-1.1) 		119.1
(-3) 		220.3
(-0.3) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 374
1 2 3 4 5 6 range
A 340.7
(3)
P 		196.4
(-0.1)
P 		104.5
(-2.1)
P 		68.6
(-2.9)
A 		65.3
(-3)
P 		123.9
(-1.7)
P 		275
B 439.7
(3)
P 		287.1
(0.4)
P 		126.5
(-2.3)
P 		83.2
(-3)
M 		120.1
(-2.4)
P 		143
(-2)
P 		357
C 426.4
(3)
P 		268.6
(0.2)
P 		96.7
(-2.9)
M 		89.8
(-3)
A 		89.7
(-3)
P 		134.9
(-2.2)
P 		337
avr 402.3
(3) 		250.7
(0.2) 		109.2
(-2.5) 		80.5
(-3) 		91.7
(-2.8) 		133.9
(-2) 		322
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 121.2
(3)
P 		40.2
(-2.2)
P 		27.2
(-3)
P 		30.6
(-2.8)
P 		94
B 111.1
(3)
P 		57.8
(-0.8)
P 		26.7
(-3)
P 		51.1
(-1.3)
P 		84
C 115
(3)
P 		44.5
(-2.3)
P 		48.1
(-2.1)
M 		35.8
(-3)
P 		79
avr 115.8
(3) 		47.5
(-2) 		34
(-3) 		39.2
(-2.6) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 62.8
(2.2)
P 		69.8
(3)
P 		22.8
(-2.4)
P 		17.4
(-3)
A 		19.7
(-2.7)
P 		30.2
(-1.5)
P 		52
B 153
(3)
P 		95.6
(0.4)
P 		30.7
(-2.6)
P 		22
(-3)
M 		48.8
(-1.8)
P 		53.3
(-1.6)
P 		131
C 158
(3)
P 		82
(-0.3)
P 		24.6
(-2.7)
M 		18
(-3)
A 		29
(-2.5)
P 		45.2
(-1.8)
P 		140
avr 124.6
(3) 		82.5
(0.6) 		26
(-2.6) 		19.1
(-3) 		32.5
(-2.2) 		42.9
(-1.6) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174167_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20868
Sequence Type Exemplar sequence
Representative Public ID C31686 NCBI
Target Description C31686_rc /REP_DB=TREMBL Accession /GB=C31686 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk290e1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062286(11), NM_062287(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062286 NCBI Caenorhabditis elegans F53C3.6a (F53C3.6) mRNA, complete cds. 11/11 A 
NM_062287 NCBI Caenorhabditis elegans F53C3.6b (F53C3.6) mRNA, complete cds. 10/11 None
SNAP00000027221 ENSEMBL cdna:SNAP chromosome:CEL140:II:3898964:3899559:1 10/11 None
F53C3.6a.2 ENSEMBL cdna:known chromosome:CEL140:II:3898128:3899559:1 gene:F53C3.6 10/11 None
F53C3.6a.1 ENSEMBL cdna:known chromosome:CEL140:II:3898135:3899656:1 gene:F53C3.6 11/11 None
F53C3.6b ENSEMBL cdna:known chromosome:CEL140:II:3898149:3899559:1 gene:F53C3.6 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000027238 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3899255-3899645(+) 89.67 100.0
Public Domain and Genome References
Ensembl F53C3.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73869 Hypothetical protein F53C3.6a [Caenorhabditis elegans] ref|NP_494687.2| F53C3.6a [Caenorhabditis elegans] 1.0E-104
blast AAK73870 Hypothetical protein F53C3.6b [Caenorhabditis elegans] ref|NP_494688.1| F53C3.6b [Caenorhabditis elegans] 6.0E-90
blast AAK73869 Hypothetical protein F53C3.6a [Caenorhabditis elegans] ref|NP_494687.2| F53C3.6a [Caenorhabditis elegans] 6.0E-90
blast AAK73870 Hypothetical protein F53C3.6b [Caenorhabditis elegans] ref|NP_494688.1| F53C3.6b [Caenorhabditis elegans] 3.0E-89
Protein Domains
Database ID Description E-Value
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 6.7E-28
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 6.7E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494687.2 1 187-206
NP_494688.1 1 88-107
F53C3.6a.2 1 187-206
F53C3.6a.1 1 187-206
F53C3.6b 1 88-107
SNAP00000027221 1 88-107
Sequence
Target Sequence 
>C. ELEGANS:174167_AT
tggagtattcctgacggcaggcctctgcttgttgatccgcagatgggtcagacaaaaagg
atattgtaacggcaaaatgacttgcaaaagagacagtcggccgacatcgggtccagaatt
tcaagaactaactcacagagctcatggtgatacaaatcgtgtcaacacttcactgaaaac
atgatatatatatttatatattatagttttttatctgttgcattcctactttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGTATTCCTGACGGCAGGCCTC 174 549 42 Antisense
GCCTCTGCTTGTTGATCCGCAGATG 47 281 62 Antisense
GCTTGTTGATCCGCAGATGGGTCAG 636 303 68 Antisense
GATCCGCAGATGGGTCAGACAAAAA 316 417 75 Antisense
GCAAAAGAGACAGTCGGCCGACATC 342 319 125 Antisense
GGCCGACATCGGGTCCAGAATTTCA 476 541 140 Antisense
GAATTTCAAGAACTAACTCACAGAG 486 325 157 Antisense
GAACTAACTCACAGAGCTCATGGTG 618 347 166 Antisense
GCTCATGGTGATACAAATCGTGTCA 2 301 181 Antisense
ATCGTGTCAACACTTCACTGAAAAC 545 35 197 Antisense
TTTTATCTGTTGCATTCCTACTTTC 657 675 250 Antisense
Sequence Source Affymetrix Proprietary Database