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[174159_at] Aminopeptidase

Description C30372_rc /REP_DB=TREMBL Accession /GB=C30372 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk246f6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 17      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 70      6grp_total > 16      4grp_total > 45      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 432
1 2 3 4 range
A 515.2
(3)
P 		314.7
(-0)
P 		242
(-1.1)
P 		114.7
(-3)
P 		401
B 539.6
(3)
P 		355.8
(0.4)
P 		217.1
(-1.5)
P 		107.7
(-3)
P 		432
C 487
(3)
P 		403.1
(1.4)
P 		287.2
(-0.9)
P 		181.9
(-3)
P 		305
avr 513.9
(3) 		357.9
(0.5) 		248.8
(-1.2) 		134.8
(-3) 		379

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 480
1 2 3 4 5 6 range
A 547.9
(3)
P 		360.8
(0.3)
P 		253.9
(-1.2)
P 		127.2
(-3)
P 		172.4
(-2.4)
P 		149.8
(-2.7)
P 		421
B 607
(3)
P 		388.3
(0.2)
P 		197.2
(-2.2)
P 		142.5
(-2.9)
P 		132.6
(-3)
P 		198.9
(-2.2)
P 		474
C 567.9
(3)
P 		334.4
(-0.3)
P 		190
(-2.4)
P 		199.5
(-2.3)
P 		149.1
(-3)
P 		203.4
(-2.2)
P 		419
avr 574.3
(3) 		361.2
(-0) 		213.7
(-2.1) 		156.4
(-2.9) 		151.4
(-3) 		184
(-2.5) 		423
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 187.6
(3)
P 		68.3
(-1.2)
P 		63.3
(-1.4)
P 		16.8
(-3)
P 		171
B 172.1
(3)
P 		87.9
(-0.4)
P 		50.3
(-1.9)
P 		22.9
(-3)
P 		149
C 161.2
(3)
P 		115.9
(0.9)
P 		100.1
(0.2)
P 		30.7
(-3)
P 		131
avr 173.6
(3) 		90.7
(-0.3) 		71.2
(-1.1) 		23.5
(-3) 		150

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 179
1 2 3 4 5 6 range
A 101
(1.3)
P 		128.3
(3)
P 		55.3
(-1.6)
P 		32.2
(-3)
P 		52
(-1.8)
P 		36.5
(-2.7)
P 		96
B 211.2
(3)
P 		129.3
(0.2)
P 		47.9
(-2.6)
P 		37.7
(-3)
P 		53.9
(-2.4)
P 		74.2
(-1.7)
P 		174
C 210.5
(3)
P 		102
(-0.8)
P 		48.4
(-2.7)
P 		40.1
(-3)
P 		48.2
(-2.7)
P 		68.1
(-2)
P 		170
avr 174.2
(3) 		119.9
(0.6) 		50.5
(-2.4) 		36.7
(-3) 		51.4
(-2.4) 		59.6
(-2) 		138
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174159_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21119
Sequence Type Exemplar sequence
Representative Public ID C30372 NCBI
Target Description C30372_rc /REP_DB=TREMBL Accession /GB=C30372 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk246f6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065477(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065477 NCBI Caenorhabditis elegans B0285.7 (Aminopeptidase) mRNA, complete cds. 11/11 None
B0285.7 ENSEMBL cdna:known chromosome:CEL140:III:4356508:4364941:1 gene:B0285.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4364563-4364866(+) 100.0 100.0
Public Domain and Genome References
Gene Title Aminopeptidase
Ensembl B0285.7
Entrez Gene 175564 Entrez gene
SwissProt P46557 EMBL-EBI
WormBase CE00646 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4179 membrane alanyl aminopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84298 Hypothetical protein B0285.7 [Caenorhabditis elegans] ref|NP_497878.1| B0285.7 [Caenorhabditis elegans] sp|P46557|YKG7_CAEEL Hypothetical protein B0285.7 in chromosome III precursor 0.0
blast CAE71220 Hypothetical protein CBG18084 [Caenorhabditis briggsae] 0.0
blast AAF55912 CG31233-PA [Drosophila melanogaster] gb|AAM50162.1| GH12469p [Drosophila melanogaster] ref|NP_732653.2| CG31233-PA [Drosophila melanogaster] 6.0E-9
Protein Domains
Database ID Description E-Value
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 3.0E-110
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497878.1 1 16-38
B0285.7 1 16-38
Sequence
Target Sequence 
>C. ELEGANS:174159_AT
gaatttgtttgagcccgggtaatgctttgaagccgaaatttgaacccacctaatatttca
tttttttccctttgattgtgatcaatgtattttcttggctcattttgcagccgacaaatt
gcgtccgccgctcagttcagttatttctttttctcccatcccatcacttttgatactcat
gttccatttttgcctgaatcttgtttgctcaacatttccaaaattgttcaaaatctcatt
tgagagcttctgtctgtcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTTGTTTGAGCCCGGGTAATGC 557 321 25 Antisense
GAGCCCGGGTAATGCTTTGAAGCCG 319 381 35 Antisense
TGAAGCCGAAATTTGAACCCACCTA 127 549 52 Antisense
TGAACCCACCTAATATTTCATTTTT 34 575 65 Antisense
CATTTTTTTCCCTTTGATTGTGATC 623 215 83 Antisense
GTATTTTCTTGGCTCATTTTGCAGC 571 453 111 Antisense
TTTGCAGCCGACAAATTGCGTCCGC 665 665 128 Antisense
CCCATCCCATCACTTTTGATACTCA 449 259 179 Antisense
GTTCCATTTTTGCCTGAATCTTGTT 394 441 205 Antisense
GAATCTTGTTTGCTCAACATTTCCA 394 327 220 Antisense
CATTTGAGAGCTTCTGTCTGTCATT 278 215 261 Antisense
Sequence Source Affymetrix Proprietary Database