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[174125_at]

Description C38014_rc /REP_DB=TREMBL Accession /GB=C38014 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk484f8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 65      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 37      6grp_total > 56      4grp_total > 57      6grp_cgc > 46      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4089
1 2 3 4 range
A 6608.8
(-1.2)
P 		8452.7
(1.6)
P 		9375.2
(3)
P 		5454.9
(-3)
P 		3920
B 7303.9
(-1.8)
P 		8817.1
(1.4)
P 		9543.4
(3)
P 		6768.3
(-3)
P 		2775
C 6226.4
(-2.9)
P 		9079.1
(3)
P 		7884.9
(0.5)
P 		6155.9
(-3)
P 		2923
avr 6713
(-1.7) 		8783
(2.7) 		8934.5
(3) 		6126.4
(-3) 		2808

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3138
1 2 3 4 5 6 range
A 5925.5
(1.7)
P 		5014.4
(-1.3)
P 		6291.5
(2.8)
P 		4469.8
(-3)
P 		5029.6
(-1.2)
P 		6341.7
(3)
P 		1872
B 5703.3
(0.9)
P 		5291.9
(-0.2)
P 		5826.6
(1.3)
P 		5455.8
(0.3)
P 		4278.5
(-3)
P 		6447.8
(3)
P 		2169
C 4700.9
(-1.8)
P 		5208.6
(-0.9)
P 		5855.3
(0.4)
P 		5406.4
(-0.5)
P 		4086.6
(-3)
P 		7224.9
(3)
P 		3138
avr 5443.2
(-0.3) 		5171.6
(-1.1) 		5991.1
(1.1) 		5110.7
(-1.2) 		4464.9
(-3) 		6671.5
(3) 		2207
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1950
1 2 3 4 range
A 2406.3
(2.8)
P 		1834.8
(0.8)
P 		2450.9
(3)
P 		798.5
(-3)
P 		1652
B 2329.2
(3)
P 		2177.6
(2)
P 		2211.4
(2.2)
P 		1442.3
(-3)
P 		887
C 2061.1
(0.6)
P 		2610.3
(2.5)
P 		2748.1
(3)
P 		1038
(-3)
P 		1710
avr 2265.5
(2.1) 		2207.6
(1.9) 		2470.1
(3) 		1092.9
(-3) 		1377

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1331
1 2 3 4 5 6 range
A 1092.3
(-3)
P 		1783.4
(3)
P 		1370.2
(-0.6)
P 		1133.6
(-2.6)
P 		1517.7
(0.7)
P 		1544
(0.9)
P 		691
B 1984.5
(0.5)
P 		1761.5
(-0.9)
P 		1414.9
(-3)
P 		1445.1
(-2.8)
P 		1738.3
(-1)
P 		2404.3
(3)
P 		989
C 1742.4
(-0)
P 		1589.3
(-0.7)
P 		1490.3
(-1.2)
P 		1087.2
(-3)
P 		1322.4
(-1.9)
P 		2418.6
(3)
P 		1331
avr 1606.4
(-0.4) 		1711.4
(0.3) 		1425.1
(-1.6) 		1222
(-3) 		1526.1
(-1) 		2122.3
(3) 		900
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174125_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20492
Sequence Type Exemplar sequence
Representative Public ID C38014 NCBI
Target Description C38014_rc /REP_DB=TREMBL Accession /GB=C38014 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk484f8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026814(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026814 NCBI Caenorhabditis elegans HELicase family member (hel-1) (hel-1) mRNA, complete cds. 10/11 None
C26D10.2a ENSEMBL cdna:known chromosome:CEL140:II:8326775:8328792:-1 gene:C26D10.2 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000007135 1/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174333 2 1 NM_001026814 173455_s_at (11), 174125_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8326792-8327172(-) 98.77 98.77
Public Domain and Genome References
Ensembl C26D10.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91120 Hypothetical protein C26D10.2a [Caenorhabditis elegans] ref|NP_001021985.1| HELicase family member (hel-1) [Caenorhabditis elegans] sp|Q18212|UAP56_CAEEL Spliceosome RNA helicase BAT1 homolog (DEAD-box protein UAP56) 0.0
blast CAE57692 Hypothetical protein CBG00694 [Caenorhabditis briggsae] 0.0
blast AAB65852 putative RNA helicase 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.5E-28
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.3E-67
Sequence
Target Sequence 
>C. ELEGANS:174125_AT
aaagatcgattcgaaggccgaaccaattgattccatgtaccctcctatgcattcaactaa
atatgagttccaccttcgttttacccggcattgtgctgatggagtaaaatgcctattgtt
tcccctaaattatcgacctctgtttcttgtggtctcatttaccaattttcgttatcgtta
acagtttccaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGATCGATTCGAAGGCCGAACCA 158 123 82 Antisense
AACCAATTGATTCCATGTACCCTCC 226 149 102 Antisense
TAAATATGAGTTCCACCTTCGTTTT 345 641 139 Antisense
TCGTTTTACCCGGCATTGTGCTGAT 619 603 157 Antisense
ACCCGGCATTGTGCTGATGGAGTAA 413 89 164 Antisense
GAGTAAAATGCCTATTGTTTCCCCT 420 397 183 Antisense
GCCTATTGTTTCCCCTAAATTATCG 35 281 192 Antisense
AAATTATCGACCTCTGTTTCTTGTG 647 113 208 Antisense
CTCTGTTTCTTGTGGTCTCATTTAC 114 231 219 Antisense
GTCTCATTTACCAATTTTCGTTATC 701 467 233 Antisense
TCGTTATCGTTAACAGTTTCCAACT 192 605 250 Antisense
Sequence Source Affymetrix Proprietary Database