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[174123_s_at]

Description C34473_rc /REP_DB=TREMBL Accession /GB=C34473 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk402c8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 83      6grp_total > 67      4grp_total > 02      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 835
1 2 3 4 range
A 464.3
(-3)
P 		497.1
(-2.8)
P 		558.4
(-2.3)
P 		1253.8
(3)
P 		790
B 428.7
(-2.9)
P 		491.6
(-2.4)
P 		418.9
(-3)
P 		1206.9
(3)
P 		788
C 459.5
(-3)
P 		531.1
(-2.4)
P 		506.3
(-2.6)
P 		1236.4
(3)
P 		777
avr 450.8
(-3) 		506.6
(-2.6) 		494.5
(-2.7) 		1232.4
(3) 		782

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 324
1 2 3 4 5 6 range
A 481.1
(-1.3)
P 		470.1
(-1.5)
P 		556.2
(0.3)
P 		520
(-0.4)
P 		398.9
(-3)
P 		683.4
(3)
P 		285
B 502.5
(-0.5)
P 		368.4
(-3)
P 		521.2
(-0.2)
P 		416.9
(-2.1)
P 		497.1
(-0.6)
P 		692.6
(3)
P 		324
C 520.2
(0.2)
P 		396.6
(-3)
P 		483.5
(-0.7)
P 		411.3
(-2.6)
P 		411.3
(-2.6)
P 		626.5
(3)
P 		230
avr 501.3
(-0.9) 		411.7
(-3) 		520.3
(-0.5) 		449.4
(-2.1) 		435.8
(-2.4) 		667.5
(3) 		256
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 169.1
(1.9)
P 		107.9
(-3)
P 		146
(0)
P 		183.5
(3)
P 		76
B 136.7
(-1.5)
P 		121.4
(-2.1)
P 		97.1
(-3)
P 		257.2
(3)
P 		160
C 152.1
(-3)
P 		152.7
(-2.9)
P 		176.5
(-0.4)
P 		208.5
(3)
P 		56
avr 152.6
(-1.3) 		127.3
(-3) 		139.9
(-2.2) 		216.4
(3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 88.7
(-3)
P 		167.2
(3)
P 		121.1
(-0.5)
P 		131.9
(0.3)
P 		120.4
(-0.6)
P 		166.4
(2.9)
P 		79
B 174.8
(-0.4)
P 		122.6
(-2.5)
P 		126.6
(-2.3)
P 		110.4
(-3)
P 		202
(0.7)
P 		258.3
(3)
P 		148
C 192.8
(2.2)
P 		121
(-1.2)
P 		123.1
(-1.1)
P 		82.7
(-3)
P 		133.1
(-0.6)
P 		209.7
(3)
P 		127
avr 152.1
(-0.5) 		136.9
(-1.3) 		123.6
(-2.1) 		108.3
(-3) 		151.8
(-0.5) 		211.5
(3) 		103
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174123_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21428
Sequence Type Exemplar sequence
Representative Public ID C34473 NCBI
Target Description C34473_rc /REP_DB=TREMBL Accession /GB=C34473 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk402c8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069982(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069982 NCBI Caenorhabditis elegans C25G4.11 (C25G4.11) mRNA, complete cds. 11/11 None
GENEFINDER00000033059 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12468641:12472449:1 11/11 None
C25G4.11 ENSEMBL cdna:known chromosome:CEL140:IV:12468641:12470210:1 gene:C25G4.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12469921-12470206(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25G4.11
Entrez Gene 178198 Entrez gene
SwissProt Q95ZY5 EMBL-EBI
WormBase CE28207 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94577 Hypothetical protein C25G4.10 [Caenorhabditis elegans] emb|CAB05615.2| Hypothetical protein C25G4.10 [Caenorhabditis elegans] ref|NP_502384.1| C25G4.10 [Caenorhabditis elegans] 0.0
blast CAE59866 Hypothetical protein CBG03342 [Caenorhabditis briggsae] 0.0
blast CAB05606 Hypothetical protein T04A11.3 [Caenorhabditis elegans] ref|NP_502387.1| T04A11.3 [Caenorhabditis elegans] 0.0
blast CAC42260 Hypothetical protein C25G4.11 [Caenorhabditis elegans] ref|NP_502383.1| C25G4.11 [Caenorhabditis elegans] 1.0E-172
blast CAE59865 Hypothetical protein CBG03341 [Caenorhabditis briggsae] 1.0E-160
Protein Domains
Database ID Description E-Value
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.1E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.3E-14
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 5.3E-39
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 1.2E-32
Sequence
Target Sequence 
>C. ELEGANS:174123_S_AT
gttcattgctgcaagactcagctcgttccgagcttctgctacgatttctgctcgggagac
ttccagatgctccgtcgatcacatcgtctctgcctctactatcttccagagatattctcg
tgtttggatcgtgccta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCATTGCTGCAAGACTCAGCTCG 658 443 14 Antisense
TGCTGCAAGACTCAGCTCGTTCCGA 121 581 20 Antisense
AGACTCAGCTCGTTCCGAGCTTCTG 694 71 27 Antisense
TCAGCTCGTTCCGAGCTTCTGCTAC 324 625 31 Antisense
GAGCTTCTGCTACGATTTCTGCTCG 374 389 43 Antisense
TCTGCTACGATTTCTGCTCGGGAGA 129 609 48 Antisense
CGATTTCTGCTCGGGAGACTTCCAG 580 247 55 Antisense
TGCTCGGGAGACTTCCAGATGCTCC 284 581 62 Antisense
TCTCTGCCTCTACTATCTTCCAGAG 58 613 100 Antisense
ACTATCTTCCAGAGATATTCTCGTG 644 101 111 Antisense
TATTCTCGTGTTTGGATCGTGCCTA 76 663 126 Antisense
Sequence Source Affymetrix Proprietary Database