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[174116_at]

Description C34064_rc /REP_DB=TREMBL Accession /GB=C34064 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk393a2 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 92      6grp_total > 94      4grp_total > 48      6grp_high1_sub > 04_05      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 89.9
(-1.9)
P 		76.1
(-3)
A 		150.3
(3)
A 		118.6
(0.4)
A 		74
B 94.8
(-3)
P 		117.6
(3)
P 		117.2
(2.9)
A 		104.2
(-0.5)
A 		23
C 137
(1.5)
P 		149.1
(3)
P 		101.2
(-3)
P 		104.8
(-2.5)
A 		48
avr 107.2
(-3) 		114.3
(-0.3) 		122.9
(3) 		109.2
(-2.2) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 86.9
(-3)
A 		115.7
(-0.6)
A 		114.5
(-0.7)
P 		89.3
(-2.8)
A 		158.5
(3)
M 		118.7
(-0.3)
P 		72
B 127
(-0.3)
P 		136.2
(0.4)
P 		92.5
(-3)
P 		107.5
(-1.8)
P 		170.4
(3)
P 		168.4
(2.8)
P 		78
C 117.2
(-1.7)
P 		109.5
(-2.1)
M 		91.9
(-3)
M 		205.2
(3)
P 		148.4
(-0)
P 		137.2
(-0.6)
P 		113
avr 110.4
(-1.9) 		120.5
(-0.9) 		99.6
(-3) 		134
(0.5) 		159.1
(3) 		141.4
(1.2) 		60
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 32.7
(1.3)
P 		16.5
(-3)
A 		39.3
(3)
A 		17.4
(-2.8)
A 		23
B 30.2
(3)
P 		29
(2.1)
P 		27.2
(0.8)
A 		22.2
(-3)
A 		8
C 45.4
(3)
P 		42.9
(2.5)
P 		35.3
(0.8)
P 		17.7
(-3)
A 		28
avr 36.1
(3) 		29.5
(0.7) 		33.9
(2.2) 		19.1
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 16
(-3)
A 		41.1
(1.7)
A 		24.9
(-1.3)
P 		22.6
(-1.8)
A 		47.8
(3)
M 		28.9
(-0.6)
P 		32
B 44.2
(-0.2)
P 		45.3
(-0.1)
P 		22.5
(-3)
P 		28.5
(-2.2)
P 		69.2
(3)
P 		62.8
(2.2)
P 		47
C 43.4
(1.9)
P 		33.4
(-0.6)
M 		23.4
(-3)
M 		41.3
(1.4)
P 		48
(3)
P 		45.9
(2.5)
P 		25
avr 34.5
(-0.9) 		39.9
(0.1) 		23.6
(-3) 		30.8
(-1.6) 		55
(3) 		45.9
(1.3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174116_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20095
Sequence Type Exemplar sequence
Representative Public ID C34064 NCBI
Target Description C34064_rc /REP_DB=TREMBL Accession /GB=C34064 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk393a2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058674(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058674 NCBI Caenorhabditis elegans Y71F9AL.1 (Y71F9AL.1) mRNA, complete cds. 11/11 A 
SNAP00000002735 ENSEMBL cdna:SNAP chromosome:CEL140:I:2927682:2928009:1 11/11 None
GENEFINDER00000002748 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2927682:2929352:1 11/11 None
Y71F9AL.1 ENSEMBL cdna:known chromosome:CEL140:I:2925219:2928118:1 gene:Y71F9AL.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2927777-2928058(+) 80.43 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71F9AL.1
Entrez Gene 171864 Entrez gene
SwissProt Q9N4H1 EMBL-EBI
WormBase CE29909 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256881_AT expressed protein at
CANINE_2:CFA.18232.1.S1_AT similar to putative RNA methylase cfa
CANINE_2:CFA.19307.1.S1_S_AT similar to putative RNA methylase cfa
CANINE_2:CFAAFFX.2475.1.S1_S_AT similar to putative RNA methylase cfa
DROSOPHILA_2:1627676_AT dm
DROSGENOME1:141546_AT dm
CHICKEN:GGA.9240.1.S1_AT putative RNA methylase gga
HU35KSUBA:RC_AA226919_AT chromosome 6 open reading frame 75 hs
HU35KSUBA:AA479127_AT chromosome 6 open reading frame 75 hs
HG-U133A:218877_S_AT chromosome 6 open reading frame 75 hs
HG-U133_PLUS_2:218877_S_AT chromosome 6 open reading frame 75 hs
HG-FOCUS:218877_S_AT chromosome 6 open reading frame 75 hs
HG-U133A_2:218877_S_AT chromosome 6 open reading frame 75 hs
U133_X3P:G11141892_3P_A_AT chromosome 6 open reading frame 75 hs
HG-U95C:64118_AT chromosome 6 open reading frame 75 hs
HG-U95D:86095_AT chromosome 6 open reading frame 75 hs
HU35KSUBD:RC_AA431085_AT Chromosome 6 open reading frame 75 hs
HU35KSUBD:RC_AA452156_AT Chromosome 6 open reading frame 75 hs
MG-U74BV2:106539_AT RIKEN cDNA 2410075D05 gene mm
MG-U74BV2:163254_AT RIKEN cDNA 2410075D05 gene mm
MOE430B:1430920_AT RIKEN cDNA 2410075D05 gene mm
MOUSE430_2:1430920_AT RIKEN cDNA 2410075D05 gene mm
MOE430B:1428551_AT RIKEN cDNA 2410075D05 gene mm
MOUSE430_2:1428551_AT RIKEN cDNA 2410075D05 gene mm
MU19KSUBC:TC41762_AT RIKEN cDNA 2410075D05 gene (2410075D05Rik), mRNA mm
YEAST_2:1778811_AT Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6306 DNA methylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
8170 N-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF36002 Hypothetical protein Y71F9AL.1 [Caenorhabditis elegans] ref|NP_491075.2| Y71F9AL.1 [Caenorhabditis elegans] 0.0
blast CAE60585 Hypothetical protein CBG04220 [Caenorhabditis briggsae] 1.0E-176
blast AAK66027 Hypothetical protein Y71F9AM.6 [Caenorhabditis elegans] ref|NP_491076.1| Y71F9AM.6 [Caenorhabditis elegans] 1.0E-137
blast CAE60584 Hypothetical protein CBG04219 [Caenorhabditis briggsae] 1.0E-123
blast AAQ10288 putative RNA methylase [Danio rerio] ref|NP_956510.2| hypothetical protein LOC393185 [Danio rerio] 4.0E-65
blast AAF36002 Hypothetical protein Y71F9AL.1 [Caenorhabditis elegans] ref|NP_491075.2| Y71F9AL.1 [Caenorhabditis elegans] 8.0E-58
blast AAF36002 Hypothetical protein Y71F9AL.1 [Caenorhabditis elegans] ref|NP_491075.2| Y71F9AL.1 [Caenorhabditis elegans] 7.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR005595 EMBL-EBI Translocon-associated protein (TRAP), alpha subunit 1.9E-43
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000002748 2 122-144,299-321
Sequence
Target Sequence 
>C. ELEGANS:174116_AT
cttctggtacccggttattctcgagaactactgcgctgaaaaccttccaaaccatcctgc
aatggatttgatttcgaattgcgagcagccattgacaaggaaaaccagccggcggcttct
tagctaccgaaaaatccgggatcccgtgaatgatgaagcatgtgaaattcaaaagacagg
agtcagccactatcggagtgttttgttttcttctgcgaagccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCTGGTACCCGGTTATTCTCGAG 133 219 16 Antisense
TATTCTCGAGAACTACTGCGCTGAA 489 661 31 Antisense
AACTACTGCGCTGAAAACCTTCCAA 518 137 41 Antisense
ACCTTCCAAACCATCCTGCAATGGA 318 87 57 Antisense
GAATTGCGAGCAGCCATTGACAAGG 40 325 91 Antisense
GAAAACCAGCCGGCGGCTTCTTAGC 110 353 115 Antisense
GCGGCTTCTTAGCTACCGAAAAATC 607 261 127 Antisense
TCCGGGATCCCGTGAATGATGAAGC 403 593 150 Antisense
AAGACAGGAGTCAGCCACTATCGGA 451 151 188 Antisense
GCCACTATCGGAGTGTTTTGTTTTC 237 279 201 Antisense
GTTTTGTTTTCTTCTGCGAAGCCGA 458 447 215 Antisense
Sequence Source Affymetrix Proprietary Database