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[174104_at]

Description C66928 /REP_DB=TREMBL Accession /GB=C66928 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk234f4 : 5prime end, single read.
Cluster 6grp_total > 83      4grp_total > 0      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 51.7
(1.8)
A 		40.9
(-2.7)
A 		54.5
(3)
A 		40.3
(-3)
A 		14
B 43.2
(1.9)
A 		46.2
(3)
A 		29.9
(-3)
A 		33.4
(-1.7)
A 		16
C 47.1
(-1.3)
A 		25.8
(-3)
A 		59.5
(-0.3)
A 		100.8
(3)
A 		75
avr 47.3
(-0.2) 		37.6
(-3) 		48
(0) 		58.2
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 219
1 2 3 4 5 6 range
A 8.4
(-3)
A 		84.7
(-0.8)
A 		126
(0.4)
A 		214.9
(3)
P 		198.8
(2.5)
P 		63.3
(-1.4)
A 		207
B 66.9
(-3)
A 		78.8
(-2.4)
A 		109
(-0.7)
A 		126.8
(0.3)
P 		176.9
(3)
P 		131.7
(0.5)
P 		110
C 39.6
(-3)
A 		91
(-1.4)
A 		147.7
(0.5)
A 		227.4
(3)
M 		168.2
(1.1)
P 		137.7
(0.1)
A 		188
avr 38.3
(-3) 		84.8
(-1.2) 		127.6
(0.5) 		189.7
(3) 		181.3
(2.7) 		110.9
(-0.1) 		151
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 18.8
(3)
A 		8.9
(-1.6)
A 		14.2
(0.9)
A 		5.9
(-3)
A 		13
B 13.8
(3)
A 		11.4
(0.9)
A 		6.9
(-3)
A 		7.1
(-2.8)
A 		7
C 15.6
(0.7)
A 		7.4
(-3)
A 		20.8
(3)
A 		17
(1.3)
A 		13
avr 16.1
(3) 		9.2
(-3) 		14
(1.2) 		10
(-2.3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 1.6
(-3)
A 		30.1
(-0.1)
A 		27.4
(-0.3)
A 		54.5
(2.4)
P 		60
(3)
P 		15.4
(-1.6)
A 		58
B 23.3
(-3)
A 		26.2
(-2.6)
A 		26.5
(-2.6)
A 		33.6
(-1.7)
P 		71.9
(3)
P 		49.1
(0.2)
P 		49
C 14.7
(-3)
A 		27.8
(-1)
A 		37.6
(0.5)
A 		45.7
(1.7)
M 		54.4
(3)
P 		46.1
(1.7)
A 		40
avr 13.2
(-3) 		28
(-1.2) 		30.5
(-0.9) 		44.6
(0.9) 		62.1
(3) 		36.9
(-0.1) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174104_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20904
Sequence Type Exemplar sequence
Representative Public ID C66928 NCBI
Target Description C66928 /REP_DB=TREMBL Accession /GB=C66928 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk234f4 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065207(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065207 NCBI Caenorhabditis elegans Y71H2AM.12 (Y71H2AM.12) mRNA, complete cds. 11/11 None
Y71H2AM.12 ENSEMBL cdna:known chromosome:CEL140:III:2731396:2734001:-1 gene:Y71H2AM.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2731405-2732512(-) 88.93 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.12
Entrez Gene 175389 Entrez gene
SwissProt Q9BL44 EMBL-EBI
WormBase CE27318 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
160 two-component signal transduction system (phosphorelay) inferred from electronic annotation QuickGO AmiGO
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29982 Hypothetical protein Y71H2AM.12 [Caenorhabditis elegans] ref|NP_497608.1| Y71H2AM.12 [Caenorhabditis elegans] 1.0E-108
blast AAF23246 putative Ras-like GTP-binding protein [Arabidopsis thaliana] gb|AAM60928.1| putative Ras-like GTP-binding protein [Arabidopsis thaliana] ref|NP_187601.1| Ras-related GTP-binding protein, putative [Arabidopsis thaliana] 9.0E-31
blast CAB83313 GTP-binding protein-like [Arabidopsis thaliana] gb|AAL07200.1| unknown protein [Arabidopsis thaliana] gb|AAK59629.1| unknown protein [Arabidopsis thaliana] ref|NP_195972.1| Ras-related GTP-binding protein, putative [Arabidopsis thaliana] 3.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.5E-42
Sequence
Target Sequence 
>C. ELEGANS:174104_AT
gtttccggagcacgagactagtcagattatattattgaacgagccgcgaccagtggaatc
ggcgacaaagaggtgctgtcagagatggtagttacgaggaggtgctgcattccggaatac
tctcattctgaaataaataaaaaataattaattgtaaaattgccacgcgtggcctagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTCCGGAGCACGAGACTAGTCAG 633 449 84 Antisense
ATTATTGAACGAGCCGCGACCAGTG 490 11 114 Antisense
AACGAGCCGCGACCAGTGGAATCGG 696 143 121 Antisense
CCGCGACCAGTGGAATCGGCGACAA 131 263 127 Antisense
GGAATCGGCGACAAAGAGGTGCTGT 158 531 138 Antisense
GGTGCTGTCAGAGATGGTAGTTACG 700 499 155 Antisense
GGTAGTTACGAGGAGGTGCTGCATT 651 497 170 Antisense
GAGGAGGTGCTGCATTCCGGAATAC 417 403 179 Antisense
GCATTCCGGAATACTCTCATTCTGA 524 307 190 Antisense
TAATTGTAAAATTGCCACGCGTGGC 603 633 232 Antisense
TAAAATTGCCACGCGTGGCCTAGAA 273 637 238 Antisense
Sequence Source Affymetrix Proprietary Database