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[174094_at]

Description CEC9486 /REP_DB=TREMBL Accession /GB=C13948 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk190g3 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 31      4grp_highest_8_8 > 38      4grp_high_8_8 > 04      6grp_highest_nonorm > 27      6grp_total > 34      4grp_total > 05      6grp_highest_sub > 02      6grp_cgc > 44      6grp_high1_sub > 03      6grp_cgc_sub > 02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7049
1 2 3 4 range
A 10565.1
(0.7)
P 		6395.8
(-3)
P 		7465
(-2)
P 		13072.5
(3)
P 		6677
B 8645
(-0.8)
P 		7170.7
(-2.8)
P 		7046.8
(-3)
P 		11468.5
(3)
P 		4422
C 8497
(-1.4)
P 		6976.1
(-2.7)
P 		6677.1
(-3)
P 		13444.9
(3)
P 		6768
avr 9235.7
(-0.5) 		6847.5
(-3) 		7063
(-2.8) 		12662
(3) 		5815

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4084
1 2 3 4 5 6 range
A 7436.7
(2.2)
P 		7704.5
(3)
P 		6372.6
(-1)
P 		5683.9
(-3)
P 		7325.5
(1.9)
P 		7373.5
(2)
P 		2021
B 7777.9
(0.1)
P 		8683.2
(1.5)
P 		9722.5
(3)
P 		7050.1
(-0.9)
P 		5638.2
(-3)
P 		6433.1
(-1.8)
P 		4084
C 7096.7
(1.2)
P 		7588.8
(2.9)
P 		7626.7
(3)
P 		6649.6
(-0.3)
P 		6344
(-1.4)
P 		5868.5
(-3)
P 		1758
avr 7437.1
(0.9) 		7992.2
(3) 		7907.3
(2.7) 		6461.2
(-2.9) 		6435.9
(-3) 		6558.4
(-2.5) 		1556
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2458
1 2 3 4 range
A 3846.8
(3)
P 		1388.4
(-3)
P 		1951.5
(-1.6)
P 		1913.6
(-1.7)
P 		2458
B 2756.8
(3)
P 		1771
(-2.3)
P 		1632.9
(-3)
P 		2444
(1.3)
P 		1124
C 2812.7
(3)
P 		2005.7
(-3)
P 		2327.2
(-0.6)
P 		2267
(-1.1)
P 		807
avr 3138.8
(3) 		1721.7
(-3) 		1970.5
(-1.9) 		2208.2
(-0.9) 		1417

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1553
1 2 3 4 5 6 range
A 1370.9
(-3)
P 		2740.1
(3)
P 		1387.8
(-2.9)
P 		1441.6
(-2.7)
P 		2210.5
(0.7)
P 		1795.2
(-1.1)
P 		1369
B 2706.3
(1.9)
P 		2890.3
(3)
P 		2360.9
(-0.1)
P 		1867.3
(-3)
P 		2290.7
(-0.5)
P 		2398.9
(0.1)
P 		1023
C 2630.4
(3)
P 		2315.6
(1.5)
P 		1941.1
(-0.2)
P 		1337.2
(-3)
P 		2052.8
(0.3)
P 		1964.5
(-0.1)
P 		1293
avr 2235.9
(0.7) 		2648.7
(3) 		1896.6
(-1.1) 		1548.7
(-3) 		2184.7
(0.5) 		2052.9
(-0.2) 		1100
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174094_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21009
Sequence Type Exemplar sequence
Representative Public ID CEC9486 NCBI
Target Description CEC9486 /REP_DB=TREMBL Accession /GB=C13948 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk190g3 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066984(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066984 NCBI Caenorhabditis elegans SERCA (Sarco-Endoplasmic Reticulum Calcium ATPase) family member (sca-1) (sca-1) mRNA, complete cds. 11/11 A 
K11D9.2b ENSEMBL cdna:known chromosome:CEL140:III:10811643:10816687:-1 gene:K11D9.2 11/11 None
K11D9.2a.1 ENSEMBL cdna:known chromosome:CEL140:III:10811808:10816721:-1 gene:K11D9.2 9/11 None
K11D9.2a.2 ENSEMBL cdna:known chromosome:CEL140:III:10811808:10816722:-1 gene:K11D9.2 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176512 2 1 NM_066984 174094_at (11)
NM_066985 174028_at (11), 188973_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10811648-10812163(-) 83.69 100.0
Public Domain and Genome References
Ensembl K11D9.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07263 Hypothetical protein K11D9.2b [Caenorhabditis elegans] ref|NP_499385.3| SERCA (Sarco-Endoplasmic Reticulum Calcium ATPase) family member (sca-1) [Caenorhabditis elegans] 0.0
blast CAB07262 Hypothetical protein K11D9.2a [Caenorhabditis elegans] ref|NP_499386.1| SERCA (Sarco-Endoplasmic Reticulum Calcium ATPase) family member (sca-1) [Caenorhabditis elegans] emb|CAA09985.1| calcium ATPase [Caenorhabditis elegans] 0.0
blast CAE71397 Hypothetical protein CBG18305 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A1A2_HUMAN (P50993) Sodium/potassium-transporting ATPase alpha-2 chain precursor (EC 3.6.3.9) (Sodium pump 2) (Na+/K+ ATPase 2) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 2.3E-81
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 1.0E-126
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.9E-15
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 9.2E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499385.3 7 59-77,87-109,262-281,301-323,762-784,838-860,932-954
K11D9.2b 7 59-77,87-109,262-281,301-323,762-784,838-860,932-954
K11D9.2a.1 9 59-77,87-109,262-281,301-323,762-784,838-860,933-955,965-987,1007-1029
K11D9.2a.2 9 59-77,87-109,262-281,301-323,762-784,838-860,933-955,965-987,1007-1029
Sequence
Target Sequence 
>C. ELEGANS:174094_AT
cccatctcatttttcagactctttcatattttctaagttttccaattttttttcttttgt
agtgcgatcgttttcgtttcgagacccgaaatcgaaaggatctcttttagagatctttag
gatcttttttctttgctcaactcatcattctttgttttttcttctatatcctcttgttga
cggtgatcagacaaatttgttagaaatattattacatttcctttaggtttcttctattaa
aaaaaaagaaaacttctgctaaattcgtgtacgttgtctctcccatttctcattaaaaat
cgatattaattgtaatttttggtttgtcctccagtgtcgtgtgcgccatcgatggaaata
aaaaagtttcaaaactattatagcttttctttttatgaaaaatataaaatacaattagaa
tgtttttgttaaatgcgatacggtgtgcgcctttaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCATCTCATTTTTCAGACTCTTTC 228 259 22 Antisense
AGTGCGATCGTTTTCGTTTCGAGAC 232 59 82 Antisense
TTAGGATCTTTTTTCTTTGCTCAAC 230 681 138 Antisense
GCTCAACTCATCATTCTTTGTTTTT 693 301 156 Antisense
TTTTTTCTTCTATATCCTCTTGTTG 615 675 176 Antisense
TACATTTCCTTTAGGTTTCTTCTAT 498 643 234 Antisense
TAAATTCGTGTACGTTGTCTCTCCC 555 641 281 Antisense
GTCTCTCCCATTTCTCATTAAAAAT 193 469 297 Antisense
GTAATTTTTGGTTTGTCCTCCAGTG 226 461 333 Antisense
TGTCGTGTGCGCCATCGATGGAAAT 661 557 356 Antisense
AATGCGATACGGTGTGCGCCTTTAA 290 165 453 Antisense
Sequence Source Affymetrix Proprietary Database