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[174080_s_at] Ribosomal Proteins L2 /// ---

Description C66975 /REP_DB=TREMBL Accession /GB=C66975 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk235c12 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 31      4grp_highest_8_8 > 05      4grp_high_8_8 > 29      6grp_highest_nonorm > 09      6grp_total > 43      4grp_total > 34      6grp_cgc > 80      6grp_high1_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18907
1 2 3 4 range
A 33362.7
(-3)
P 		51286.3
(3)
P 		42758.8
(0.1)
P 		50479.5
(2.7)
P 		17924
B 34146.8
(-3)
P 		41581
(2.8)
P 		41340.1
(2.6)
P 		41861.1
(3)
P 		7714
C 32379.3
(-3)
P 		43114.4
(2.1)
P 		35975.4
(-1.3)
P 		45057.6
(3)
P 		12678
avr 33296.3
(-3) 		45327.2
(2.8) 		40024.8
(0.2) 		45799.4
(3) 		12503

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13499
1 2 3 4 5 6 range
A 29795.3
(1.8)
P 		26407
(-0.9)
P 		31348.1
(3)
P 		23793.4
(-3)
P 		24830.8
(-2.2)
P 		24247.4
(-2.6)
P 		7555
B 28798.3
(-0.8)
P 		28658.4
(-0.8)
P 		36115
(3)
P 		26939.5
(-1.7)
P 		24458.5
(-3)
P 		27295.7
(-1.5)
P 		11657
C 26677
(2)
P 		27477.2
(3)
P 		27253.2
(2.7)
P 		22615.7
(-3)
P 		27231
(2.7)
P 		24400.6
(-0.8)
P 		4862
avr 28423.5
(0.3) 		27514.2
(-0.4) 		31572.1
(3) 		24449.5
(-3) 		25506.8
(-2.1) 		25314.6
(-2.3) 		7123
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5149
1 2 3 4 range
A 12147.4
(3)
P 		11132.9
(1.7)
P 		11178
(1.8)
P 		7389.2
(-3)
P 		4758
B 10889.2
(3)
P 		10269.6
(1.1)
P 		9579.4
(-1)
P 		8920.6
(-3)
P 		1969
C 10718.5
(0.8)
P 		12395.6
(2.8)
P 		12538.5
(3)
P 		7597.4
(-3)
P 		4941
avr 11251.7
(3) 		11266
(3) 		11098.6
(2.7) 		7969.1
(-3) 		3297

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5630
1 2 3 4 5 6 range
A 5492.5
(-3)
P 		9391.6
(3)
P 		6827
(-0.9)
P 		6034.6
(-2.2)
P 		7492.9
(0.1)
P 		5903.5
(-2.4)
P 		3899
B 10020.4
(2.7)
P 		9539.2
(1.7)
P 		8769.8
(0.2)
P 		7135.3
(-3)
P 		9937.2
(2.5)
P 		10178.4
(3)
P 		3043
C 9888
(3)
P 		8384.1
(1.3)
P 		6936.4
(-0.3)
P 		4548
(-3)
P 		8811.6
(1.8)
P 		8168.3
(1.1)
P 		5340
avr 8467
(1.8) 		9105
(3) 		7511.1
(0) 		5906
(-3) 		8747.2
(2.3) 		8083.4
(1.1) 		3199
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174080_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21803
Sequence Type Exemplar sequence
Representative Public ID C66975 NCBI
Target Description C66975 /REP_DB=TREMBL Accession /GB=C66975 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk235c12 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026174(11), NM_075539(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026174 NCBI Caenorhabditis elegans B0250.3 (B0250.3) mRNA, complete cds. 11/11 None
NM_075539 NCBI Caenorhabditis elegans Ribosomal Protein, Large subunit family member (rpl-2) (rpl-2) mRNA, complete cds. 11/11 A 
SNAP00000043584 ENSEMBL cdna:SNAP chromosome:CEL140:V:20466400:20467464:-1 11/11 None
SNAP00000043590 ENSEMBL cdna:SNAP chromosome:CEL140:V:20477746:20481281:1 11/11 None
GENEFINDER00000043596 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20466400:20468536:-1 11/11 None
GENEFINDER00000043597 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20477746:20478810:1 11/11 None
B0250.3 ENSEMBL cdna:known chromosome:CEL140:V:20466400:20467222:-1 gene:B0250.3 11/11 None
B0250.1.1 ENSEMBL cdna:known chromosome:CEL140:V:20477735:20478859:1 gene:B0250.1 11/11 None
B0250.1.2 ENSEMBL cdna:known chromosome:CEL140:V:20477740:20478859:1 gene:B0250.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20478647-20478849(+) 100.0 100.0
chrV:20466360-20466562(-) 100.0 100.0
Public Domain and Genome References
Gene Title Ribosomal Proteins L2

Gene Symbol rpl-2
Ensembl B0250.3
B0250.1
Entrez Gene 180341 Entrez gene
180343 Entrez gene
SwissProt Q9XVF7 EMBL-EBI
Q6BER9 EMBL-EBI
WormBase CE18478 Wormbase
CE36578 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:265805_S_AT 60S ribosomal protein L8 (RPL8A) at
ATGENOME1:16924_S_AT 60S ribosomal protein L8 (RPL8A) at
CANINE:1587333_AT similar to 60S ribosomal protein L8 cfa
CANINE_2:CFA.4446.1.S1_S_AT similar to 60S ribosomal protein L8 cfa
DROSOPHILA_2:1628775_S_AT Ribosomal protein L8 dm
DROSGENOME1:144032_AT Ribosomal protein L8 dm
CHICKEN:GGA.4650.1.S1_A_AT ribosomal protein L8 gga
HG-U95AV2:31505_AT ribosomal protein L8 hs
HG-U133_PLUS_2:200936_AT ribosomal protein L8 hs
HG-U133A_2:200936_AT ribosomal protein L8 hs
HG-U133A:200936_AT ribosomal protein L8 hs
HG-FOCUS:200936_AT ribosomal protein L8 hs
HUGENEFL:Z28407_AT ribosomal protein L8 hs
U133_X3P:G4506662_3P_AT ribosomal protein L8 hs
MU11KSUBA:U67771_F_AT ribosomal protein L8 mm
MU11KSUBB:W91634_RC_F_AT ribosomal protein L8 mm
MG-U74AV2:96575_AT ribosomal protein L8 mm
MOE430A:1417762_A_AT ribosomal protein L8 mm
MOUSE430A_2:1417762_A_AT ribosomal protein L8 mm
MOUSE430_2:1417762_A_AT ribosomal protein L8 mm
MU11KSUBB:MSA.3012.0_S_AT ribosomal protein L8 mm
MU19KSUBC:TC36162_AT Ribosomal protein L8 (Rpl8), mRNA mm
MU19KSUBC:TC36163_F_AT Ribosomal protein L8 (Rpl8), mRNA mm
MU19KSUBC:TC36163_I_AT Ribosomal protein L8 (Rpl8), mRNA mm
RAE230A:1371305_AT Ribosomal protein L8 rn
RAT230_2:1371305_AT Ribosomal protein L8 rn
YEAST_2:1773091_AT Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures Sc
YEAST_2:1780178_S_AT Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
5840 ribosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3735 structural constituent of ribosome inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03792 Hypothetical protein B0250.1 [Caenorhabditis elegans] ref|NP_507940.1| Ribosomal Protein, Large subunit family member (rpl-2) [Caenorhabditis elegans] sp|Q9XVF7|RL8_CAEEL 60S ribosomal protein L8 1.0E-140
blast CAE61654 Hypothetical protein CBG05588 [Caenorhabditis briggsae] 1.0E-138
Protein Domains
Database ID Description E-Value
Pfam IPR002171 EMBL-EBI Ribosomal protein L2 1.2E-64
Pfam IPR002171 EMBL-EBI Ribosomal protein L2 1.4E-19
Pfam IPR002171 EMBL-EBI Ribosomal protein L2 1.2E-64
Pfam IPR002171 EMBL-EBI Ribosomal protein L2 1.2E-14
Pfam IPR002171 EMBL-EBI Ribosomal protein L2 1.2E-64
Pfam IPR002171 EMBL-EBI Ribosomal protein L2 7.7E-14
Pfam IPR002171 EMBL-EBI Ribosomal protein L2 1.2E-64
Sequence
Target Sequence 
>C. ELEGANS:174080_S_AT
acatccccacggaggaggtaaccatcaacatattggacatccatccaccgtcagaagaga
cgccagtgccggaaagaaggttggacttatcgccgcccgccgtaccggaagaattcgcgg
aggaaagccagtcaagttcaccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATCCCCACGGAGGAGGTAACCAT 707 101 16 Antisense
TCCCCACGGAGGAGGTAACCATCAA 424 567 19 Antisense
GGAGGTAACCATCAACATATTGGAC 292 515 29 Antisense
ACATATTGGACATCCATCCACCGTC 657 103 43 Antisense
CATCCATCCACCGTCAGAAGAGACG 628 209 53 Antisense
GTCAGAAGAGACGCCAGTGCCGGAA 109 465 65 Antisense
GAGACGCCAGTGCCGGAAAGAAGGT 57 393 72 Antisense
GAAAGAAGGTTGGACTTATCGCCGC 243 355 87 Antisense
CGCCGTACCGGAAGAATTCGCGGAG 497 255 113 Antisense
GAATTCGCGGAGGAAAGCCAGTCAA 706 323 126 Antisense
GAGGAAAGCCAGTCAAGTTCACCAA 330 405 135 Antisense
Sequence Source Affymetrix Proprietary Database