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[174066_at] GTP-binding protein

Description C69946 /REP_DB=TREMBL Accession /GB=C69946 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk370c1 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 0      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 37      6grp_total > 90      4grp_total > 40      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3033
1 2 3 4 range
A 4625.7
(-2)
P 		4669
(-1.9)
P 		6746.7
(3)
P 		4204.6
(-3)
P 		2542
B 5035.3
(-3)
P 		5579.6
(-1.3)
P 		6710.5
(2.1)
P 		6990.2
(3)
P 		1955
C 3957.5
(-3)
P 		5590.3
(1.6)
P 		5644.2
(1.7)
P 		6097.8
(3)
P 		2140
avr 4539.5
(-3) 		5279.6
(-0.6) 		6367.1
(3) 		5764.2
(1) 		1828

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2156
1 2 3 4 5 6 range
A 5510.3
(3)
P 		4457.8
(-3)
P 		4915.1
(-0.4)
P 		5351.5
(2.1)
P 		5507.5
(3)
P 		5333.9
(2)
P 		1053
B 5925.6
(3)
P 		4828.9
(-2.2)
P 		5371.6
(0.4)
P 		5767.3
(2.3)
P 		4650.5
(-3)
P 		5140.3
(-0.7)
P 		1275
C 5015.5
(-1.8)
P 		4712.5
(-2.8)
P 		4885.5
(-2.2)
P 		6614
(3)
P 		4631.1
(-3)
P 		5107.7
(-1.6)
P 		1983
avr 5483.8
(0.9) 		4666.4
(-3) 		5057.4
(-1.1) 		5910.9
(3) 		4929.7
(-1.7) 		5194
(-0.5) 		1245
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1352
1 2 3 4 range
A 1684.2
(2.6)
P 		1013.5
(-0.9)
P 		1763.7
(3)
P 		615.5
(-3)
P 		1148
B 1605.7
(3)
P 		1378
(-3)
P 		1555
(1.7)
P 		1489.6
(-0.1)
P 		228
C 1310
(-1.2)
P 		1607.2
(0.7)
P 		1967.2
(3)
P 		1028.2
(-3)
P 		939
avr 1533.3
(1.1) 		1332.9
(-0.6) 		1762
(3) 		1044.4
(-3) 		718

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1046
1 2 3 4 5 6 range
A 1015.8
(-3)
P 		1585.4
(2.3)
P 		1070.4
(-2.5)
P 		1357.3
(0.2)
P 		1661.9
(3)
P 		1298.6
(-0.4)
P 		646
B 2061.8
(3)
P 		1607.4
(-0.6)
P 		1304.4
(-3)
P 		1527.6
(-1.2)
P 		1889.4
(1.6)
P 		1916.8
(1.9)
P 		757
C 1859
(3)
P 		1437.9
(-1.1)
P 		1243.5
(-3)
P 		1330.1
(-2.2)
P 		1498.6
(-0.5)
P 		1709.9
(1.5)
P 		616
avr 1645.5
(2.5) 		1543.6
(1.2) 		1206.1
(-3) 		1405
(-0.5) 		1683.3
(3) 		1641.8
(2.5) 		477
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174066_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21521
Sequence Type Exemplar sequence
Representative Public ID C69946 NCBI
Target Description C69946 /REP_DB=TREMBL Accession /GB=C69946 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk370c1 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068181(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068181 NCBI Caenorhabditis elegans ZK180.4 (ZK180.4) mRNA, complete cds. 11/11 None
ZK180.4 ENSEMBL cdna:known chromosome:CEL140:IV:4511889:4513314:-1 gene:ZK180.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4511923-4512273(-) 93.43 100.0
Public Domain and Genome References
Gene Title GTP-binding protein
Ensembl ZK180.4
Entrez Gene 177217 Entrez gene
SwissProt Q23445 EMBL-EBI
WormBase CE07622 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251217_AT GTP-binding protein, putative at
CANINE:1583356_S_AT similar to GTP-binding protein SAR1b (GTBPB) cfa
CANINE_2:CFA.3394.1.A1_AT similar to GTP-binding protein SAR1b (GTBPB) cfa
CANINE_2:CFAAFFX.2433.1.S1_AT similar to GTP-binding protein SAR1b (GTBPB) cfa
CANINE_2:CFAAFFX.2433.1.S1_S_AT similar to GTP-binding protein SAR1b (GTBPB) cfa
DROSOPHILA_2:1623901_S_AT dm
DROSGENOME1:154858_AT dm
HG-U133A:218254_S_AT SAR1 gene homolog B (S. cerevisiae) hs
HG-U133_PLUS_2:218254_S_AT SAR1 gene homolog B (S. cerevisiae) hs
HG-FOCUS:218254_S_AT SAR1 gene homolog B (S. cerevisiae) hs
HG-U133A_2:218254_S_AT SAR1 gene homolog B (S. cerevisiae) hs
HU35KSUBA:W67213_AT SAR1 gene homolog B (S. cerevisiae) hs
HU35KSUBD:RC_N95495_AT SAR1 gene homolog B (S. cerevisiae) hs
U133_X3P:G7705826_3P_A_AT SAR1 gene homolog B (S. cerevisiae) hs
U133_X3P:HS2.279582.2.S1_3P_S_AT SAR1 gene homolog B (S. cerevisiae) hs
HG-U133_PLUS_2:1554482_A_AT SAR1 gene homolog B (S. cerevisiae) hs
HG-U95C:65565_AT SAR1 gene homolog B (S. cerevisiae) hs
HG-U95E:82968_AT SAR1 gene homolog B (S. cerevisiae) hs
HU35KSUBD:RC_AA426380_AT SAR1a gene homolog 2 (S. cerevisiae) hs
U133_X3P:HS.279582.1.S2_3P_AT SAR1a gene homolog 2 (S. cerevisiae) hs
HG-U133_PLUS_2:223512_AT SAR1a gene homolog 2 (S. cerevisiae) hs
HG-U133B:223512_AT SAR1a gene homolog 2 (S. cerevisiae) hs
HG-U95B:56030_AT SAR1a gene homolog 2 (S. cerevisiae) hs
MU11KSUBA:AA498748_G_AT SAR1a gene homolog 2 (S. cerevisiae) mm
MU11KSUBA:AA498748_AT SAR1a gene homolog 2 (S. cerevisiae) mm
MU11KSUBB:MSA.6655.0_S_AT SAR1a gene homolog 2 (S. cerevisiae) mm
MG-U74AV2:94871_R_AT SAR1a gene homolog 2 (S. cerevisiae) mm
MG-U74AV2:94870_F_AT SAR1a gene homolog 2 (S. cerevisiae) mm
MOE430A:1428163_AT SAR1a gene homolog 2 (S. cerevisiae) mm
MOUSE430A_2:1428163_AT SAR1a gene homolog 2 (S. cerevisiae) mm
MOUSE430_2:1428163_AT SAR1a gene homolog 2 (S. cerevisiae) mm
MU19KSUBB:TC33186_AT SAR1a gene homolog 2 (S. cerevisiae) (Sara2), mRNA mm
MU11KSUBA:T25544_RC_AT SAR1a gene homolog 2 (S. cerevisiae) (Sara2), mRNA mm
RG-U34C:RC_AI105294_AT SAR1a gene homolog 2 (S. cerevisiae) (predicted) rn
RAE230A:1388681_AT SAR1a gene homolog 2 (S. cerevisiae) (predicted) rn
RAT230_2:1388681_AT SAR1a gene homolog 2 (S. cerevisiae) (predicted) rn
YEAST_2:1775816_AT GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52968 Hypothetical protein ZK180.4 [Caenorhabditis elegans] ref|NP_500582.1| ZK180.4 [Caenorhabditis elegans] sp|Q23445|SAR1_CAEEL GTP-binding protein SAR1 1.0E-109
blast CAE58542 Hypothetical protein CBG01701 [Caenorhabditis briggsae] 1.0E-107
blast AAZ42354 GTP-binding subunit of SAR1 [Caenorhabditis remanei] 5.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR006689 EMBL-EBI ARF/SAR superfamily 7.9E-95
Sequence
Target Sequence 
>C. ELEGANS:174066_AT
tgtacgtacttgtcattttccttcgttttttcttttgggatccttgggatcctcatcatt
aaacttctcatgccttcgacgtttgtgcataaaaaagcaagccgcaatcccattttattt
tatttataaattttgacttatacggacttgtaaaaaattcacggcgtcctcattgttttt
ctattctccacaaaaatttattcaaaccttttcctcatctattccgtatttatcacatca
ctatcattcaaacatccccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTACGTACTTGTCATTTTCCTTCG 646 559 21 Antisense
GATCCTTGGGATCCTCATCATTAAA 20 417 59 Antisense
CATCATTAAACTTCTCATGCCTTCG 488 213 74 Antisense
CTCATGCCTTCGACGTTTGTGCATA 287 229 87 Antisense
AAAAAAGCAAGCCGCAATCCCATTT 665 129 111 Antisense
GCCGCAATCCCATTTTATTTTATTT 84 285 121 Antisense
AAATTCACGGCGTCCTCATTGTTTT 686 115 175 Antisense
ATTTATTCAAACCTTTTCCTCATCT 274 15 216 Antisense
TTTCCTCATCTATTCCGTATTTATC 607 669 230 Antisense
ATTCCGTATTTATCACATCACTATC 176 9 241 Antisense
ATCACTATCATTCAAACATCCCCAT 367 27 257 Antisense
Sequence Source Affymetrix Proprietary Database