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[174048_at]

Description C42058 /REP_DB=TREMBL Accession /GB=C42058 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk284e8 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 47      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 87      6grp_total > 47      4grp_total > 37      6grp_highest_sub > Cu      6grp_cgc > 17      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 947
1 2 3 4 range
A 1471.5
(0.7)
P 		1839.2
(3)
P 		1226.3
(-0.9)
P 		892.1
(-3)
P 		947
B 1560.8
(2.6)
P 		1322.1
(-3)
P 		1366.4
(-2)
P 		1576
(3)
P 		254
C 1378.6
(2.2)
P 		1225.4
(-0.8)
P 		1112.4
(-3)
P 		1421
(3)
P 		309
avr 1470.3
(3) 		1462.2
(2.8) 		1235
(-3) 		1296.4
(-1.4) 		235

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 995
1 2 3 4 5 6 range
A 1197.3
(-0.1)
P 		1177.1
(-0.2)
P 		863.7
(-3)
P 		890.4
(-2.8)
P 		949.4
(-2.2)
P 		1542.5
(3)
P 		679
B 1751.1
(3)
P 		1089.6
(-1.2)
P 		1082.9
(-1.2)
P 		940.8
(-2.1)
P 		801.4
(-3)
P 		1412.7
(0.9)
P 		950
C 1444
(2.7)
P 		1233.7
(0.9)
P 		966.2
(-1.3)
P 		755.8
(-3)
P 		943.5
(-1.5)
P 		1483.8
(3)
P 		728
avr 1464.1
(2.8) 		1166.8
(-0) 		970.9
(-1.9) 		862.3
(-3) 		898.1
(-2.7) 		1479.7
(3) 		617
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 405
1 2 3 4 range
A 535.8
(3)
P 		399.2
(1)
P 		320.6
(-0.2)
P 		130.6
(-3)
P 		405
B 497.7
(3)
P 		326.5
(-2.7)
P 		316.6
(-3)
P 		335.9
(-2.4)
P 		181
C 456.4
(3)
P 		352.3
(0.1)
P 		387.7
(1.1)
P 		239.6
(-3)
P 		217
avr 496.6
(3) 		359.3
(-0.2) 		341.6
(-0.6) 		235.4
(-3) 		261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 457
1 2 3 4 5 6 range
A 220.7
(-2.2)
P 		418.6
(3)
P 		188.1
(-3)
P 		225.8
(-2)
P 		286.5
(-0.4)
P 		375.6
(1.9)
P 		231
B 609.3
(3)
P 		362.7
(-1.1)
P 		263
(-2.8)
P 		249.2
(-3)
P 		325.6
(-1.7)
P 		526.8
(1.6)
P 		360
C 535.2
(3)
P 		376.5
(0.5)
P 		245.9
(-1.5)
P 		152
(-3)
P 		305.3
(-0.6)
P 		496.7
(2.4)
P 		383
avr 455.1
(2.7) 		385.9
(1.1) 		232.3
(-2.5) 		209
(-3) 		305.8
(-0.7) 		466.4
(3) 		257
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174048_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22125
Sequence Type Exemplar sequence
Representative Public ID C42058 NCBI
Target Description C42058 /REP_DB=TREMBL Accession /GB=C42058 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk284e8 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029600(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029600 NCBI Caenorhabditis elegans MAP kinase kinase or Erk Kinase family member (mek-1) (mek-1) mRNA, complete cds. 11/11 None
K08A8.1b ENSEMBL cdna:known chromosome:CEL140:X:7479193:7480869:1 gene:K08A8.1 11/11 None
K08A8.1a ENSEMBL cdna:known chromosome:CEL140:X:7479201:7480945:1 gene:K08A8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181004 2 1 NM_001029600 174048_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7480541-7480882(+) 88.56 100.0
Public Domain and Genome References
Ensembl K08A8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA79746 Map kinase kinase or erk kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024771.1| MAP kinase kinase or Erk Kinase family member (mek-1) [Caenorhabditis elegans] 0.0
blast AAP46283 Map kinase kinase or erk kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024772.1| MAP kinase kinase or Erk Kinase family member (mek-1) [Caenorhabditis elegans] 1.0E-154
blast CAE70218 Hypothetical protein CBG16698 [Caenorhabditis briggsae] 1.0E-149
blast AAP46283 Map kinase kinase or erk kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024772.1| MAP kinase kinase or Erk Kinase family member (mek-1) [Caenorhabditis elegans] 1.0E-149
blast AAA79746 Map kinase kinase or erk kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024771.1| MAP kinase kinase or Erk Kinase family member (mek-1) [Caenorhabditis elegans] 1.0E-149
blast CAE70218 Hypothetical protein CBG16698 [Caenorhabditis briggsae] 1.0E-126
Protein Families
Method ID Description E-Value
hanks 1.1.10 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); DdPK1 8.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-43
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-56
Sequence
Target Sequence 
>C. ELEGANS:174048_AT
attgttatttcccaaatcatttgtaaaaactatcaatcccattcactatctctttcataa
catttcctggtctagcattcatggtgcaaagtttttcaattttgcttttttaccttattt
accctatttaccacttttaccctattttcacttgttctcgttcccgttgtctctatcaaa
aattcccaacctctctcaccgaatccctgtctaccaaacttgaatgttccggatgtatag
tatcattgtaggcctaattgattgtatattttgattatgatatgtctcaaagttaagcca
tccacagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGTTATTTCCCAAATCATTTGTA 430 5 13 Antisense
AACTATCAATCCCATTCACTATCTC 47 141 40 Antisense
CCCATTCACTATCTCTTTCATAACA 447 259 50 Antisense
ATCTCTTTCATAACATTTCCTGGTC 543 31 60 Antisense
ATTTCCTGGTCTAGCATTCATGGTG 366 17 74 Antisense
ATTCATGGTGCAAAGTTTTTCAATT 419 11 89 Antisense
CGTTCCCGTTGTCTCTATCAAAAAT 294 247 171 Antisense
AATCCCTGTCTACCAAACTTGAATG 150 167 214 Antisense
GAATGTTCCGGATGTATAGTATCAT 206 329 234 Antisense
ATAGTATCATTGTAGGCCTAATTGA 64 23 249 Antisense
GTCTCAAAGTTAAGCCATCCACAGC 670 469 296 Antisense
Sequence Source Affymetrix Proprietary Database