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[174047_at]

Description C51214 /REP_DB=TREMBL Accession /GB=C51214 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk492e6 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 66      4grp_highest_8_8 > 15      4grp_high_8_8 > 13      6grp_highest_nonorm > 47      6grp_total > 57      4grp_total > 10      6grp_highest_sub > Cu      6grp_high1_sub > Da      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3835
1 2 3 4 range
A 4966.9
(-3)
P 		5999.2
(-0.2)
P 		5344.8
(-2)
P 		7200.2
(3)
P 		2233
B 4069.6
(-3)
P 		4963.3
(-1.4)
P 		4465.5
(-2.3)
P 		7496.9
(3)
P 		3427
C 5446.7
(-1.3)
P 		4704.1
(-2.6)
P 		4477.9
(-3)
P 		7904.1
(3)
P 		3426
avr 4827.7
(-2.9) 		5222.2
(-2) 		4762.7
(-3) 		7533.7
(3) 		2771

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1150
1 2 3 4 5 6 range
A 4016.6
(0.9)
P 		4024.5
(1)
P 		4134.5
(2.2)
P 		4025.7
(1)
P 		3670.6
(-3)
P 		4202.4
(3)
P 		532
B 3495.2
(-0.4)
P 		3052.5
(-3)
P 		3092.4
(-2.8)
P 		3258
(-1.8)
P 		3696.5
(0.8)
P 		4058.1
(3)
P 		1006
C 3604.5
(2.1)
P 		3433
(0.5)
P 		3695.6
(3)
P 		3486.1
(1)
P 		3076.7
(-3)
P 		3406.5
(0.2)
P 		619
avr 3705.4
(0.3) 		3503.3
(-2.7) 		3640.8
(-0.7) 		3589.9
(-1.4) 		3481.3
(-3) 		3889
(3) 		408
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 774
1 2 3 4 range
A 1808.5
(3)
P 		1302.3
(-1)
P 		1397.2
(-0.3)
P 		1054
(-3)
P 		755
B 1297.8
(-0.2)
P 		1225.8
(-1)
P 		1034.8
(-3)
P 		1597.6
(3)
P 		563
C 1803
(3)
P 		1352.4
(-2.7)
P 		1560.7
(-0.1)
P 		1332.7
(-3)
P 		470
avr 1636.4
(3) 		1293.5
(-3) 		1330.9
(-2.3) 		1328.1
(-2.4) 		343

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 812
1 2 3 4 5 6 range
A 740.4
(-3)
P 		1431.3
(3)
P 		900.4
(-1.6)
P 		1021
(-0.6)
P 		1107.6
(0.2)
P 		1023.1
(-0.5)
P 		691
B 1216.1
(0.7)
P 		1016
(-0.9)
P 		750.9
(-3)
P 		862.9
(-2.1)
P 		1501.8
(2.9)
P 		1513.2
(3)
P 		762
C 1336
(3)
P 		1047.5
(0.3)
P 		940.6
(-0.7)
P 		701
(-3)
P 		995.6
(-0.2)
P 		1140.4
(1.2)
P 		635
avr 1097.5
(0.9) 		1164.9
(2) 		864
(-3) 		861.6
(-3) 		1201.7
(2.6) 		1225.6
(3) 		364
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174047_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20755
Sequence Type Exemplar sequence
Representative Public ID C51214 NCBI
Target Description C51214 /REP_DB=TREMBL Accession /GB=C51214 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk492e6 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062517(11), NM_001027205(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062517 NCBI Caenorhabditis elegans T27F7.3a (T27F7.3) mRNA, complete cds. 11/11 A 
NM_001027205 NCBI Caenorhabditis elegans T27F7.3b (T27F7.3) mRNA, complete cds. 11/11 None
T27F7.3b.1 ENSEMBL cdna:known chromosome:CEL140:II:4990816:4993565:-1 gene:T27F7.3 11/11 None
T27F7.3a ENSEMBL cdna:known chromosome:CEL140:II:4990816:4993565:-1 gene:T27F7.3 10/11 None
T27F7.3b.2 ENSEMBL cdna:known chromosome:CEL140:II:4990834:4993567:-1 gene:T27F7.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4990891-4991412(-) 100.0 100.0
Public Domain and Genome References
Ensembl T27F7.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494918 T27F7.3a [Caenorhabditis elegans] gb|AAK39304.2| Hypothetical protein T27F7.3a [Caenorhabditis elegans] emb|CAD24083.1| putative alpha 2 mannosyltransferase [Caenorhabditis elegans] 0.0
blast CAE59016 Hypothetical protein CBG02293 [Caenorhabditis briggsae] 0.0
blast CAF99570 unnamed protein product [Tetraodon nigroviridis] 2.0E-63
blast NP_001022376 T27F7.3b [Caenorhabditis elegans] gb|AAK39303.1| Hypothetical protein T27F7.3b [Caenorhabditis elegans] 5.0E-58
blast AAR04678 Sui1 [Bombyx mori] 3.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR005599 EMBL-EBI Plasmid Maintenance Protein 1.0E-126
Pfam IPR001950 EMBL-EBI Translation initiation factor SUI1 3.9E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494918.2 7 7-26,167-189,202-224,256-278,285-304,309-326,335-357
T27F7.3a 7 7-26,167-189,202-224,256-278,285-304,309-326,335-357
Sequence
Target Sequence 
>C. ELEGANS:174047_AT
cttcctcatcaaggtcggtatcgtaaatgagtccaactgcagagtgcacggtttctaagc
tccaaactccaaaaactgaccaaaatgatgatgacgccgtacttttctctcccaatcttg
cttcctttttgaagtattgagttccctttctatttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCCTCATCAAGGTCGGTATCGTA 540 219 89 Antisense
GGTCGGTATCGTAAATGAGTCCAAC 310 503 101 Antisense
AAATGAGTCCAACTGCAGAGTGCAC 9 121 113 Antisense
GCAGAGTGCACGGTTTCTAAGCTCC 214 313 127 Antisense
TGCACGGTTTCTAAGCTCCAAACTC 323 577 133 Antisense
TTCTAAGCTCCAAACTCCAAAAACT 351 691 141 Antisense
GCTCCAAACTCCAAAAACTGACCAA 428 297 147 Antisense
AACTGACCAAAATGATGATGACGCC 83 143 162 Antisense
TGATGATGACGCCGTACTTTTCTCT 91 565 174 Antisense
TCCCAATCTTGCTTCCTTTTTGAAG 28 599 198 Antisense
AGTATTGAGTTCCCTTTCTATTTCG 556 61 221 Antisense
Sequence Source Affymetrix Proprietary Database