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[174011_at]

Description C48513 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T14G12.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C48513 /WB_GENE_ID=T14G12.4 /WP=CE04965 /CHR=X /FEA=Genomic Cluster /DEF=fork head domain transcription factor (SLP2, D. melanogaster); C.elegans cDNA clone yk457e12 : 5prime end, single read.
Cluster 6grp_total > 37      4grp_total > 37      6grp_cgc > 16      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 81.1
(2.4)
M 		85.2
(3)
A 		40.6
(-3)
A 		52.2
(-1.4)
A 		45
B 90.1
(3)
A 		54.2
(-0.8)
M 		34.1
(-3)
A 		73
(1.2)
A 		56
C 55.5
(-2.1)
P 		81.3
(3)
A 		51.2
(-3)
A 		61.6
(-0.9)
A 		30
avr 75.6
(3) 		73.6
(2.6) 		42
(-3) 		62.3
(0.6) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 177.9
(3)
P 		65.3
(-1.4)
A 		60.3
(-1.6)
A 		23.5
(-3)
A 		61.8
(-1.5)
A 		79.3
(-0.8)
A 		154
B 102.6
(2.2)
P 		78.4
(-0.4)
A 		63.6
(-1.9)
A 		64.4
(-1.8)
A 		52.8
(-3)
A 		110.8
(3)
P 		58
C 95.3
(1.8)
M 		116.8
(3)
M 		42.7
(-1.1)
A 		84
(1.2)
A 		7.5
(-3)
A 		93.9
(1.7)
M 		109
avr 125.3
(3) 		86.8
(0.3) 		55.5
(-2) 		57.3
(-1.8) 		40.7
(-3) 		94.7
(0.8) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 29.5
(3)
M 		18.5
(-0)
A 		10.6
(-2.2)
A 		7.6
(-3)
A 		22
B 28.7
(3)
A 		13.4
(-1.4)
M 		7.9
(-3)
A 		15.5
(-0.8)
A 		21
C 18.4
(0.7)
P 		23.4
(3)
A 		17.9
(0.5)
A 		10.4
(-3)
A 		13
avr 25.5
(3) 		18.4
(0) 		12.1
(-2.6) 		11.2
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 32.8
(3)
P 		23.2
(0.9)
A 		13.1
(-1.4)
A 		6
(-3)
A 		18.7
(-0.2)
A 		19.3
(-0)
A 		27
B 35.7
(1.7)
P 		26.1
(-0.5)
A 		15.4
(-3)
A 		17
(-2.6)
A 		21.4
(-1.6)
A 		41.3
(3)
P 		26
C 35.3
(2.9)
M 		35.7
(3)
M 		10.9
(-1.5)
A 		16.9
(-0.4)
A 		2.4
(-3)
A 		31.4
(2.2)
M 		33
avr 34.6
(3) 		28.3
(1.2) 		13.1
(-3) 		13.3
(-2.9) 		14.2
(-2.7) 		30.7
(1.9) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174011_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26175
Sequence Type Exemplar sequence
Representative Public ID C48513 NCBI
Target Description C48513 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T14G12.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C48513 /WB_GENE_ID=T14G12.4 /WP=CE04965 /CHR=X /FEA=Genomic Cluster /DEF=fork head domain transcription factor (SLP2, D. melanogaster); C.elegans cDNA clone yk457e12 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076243(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076243 NCBI Caenorhabditis elegans ForKHead transcription factor family member (fkh-2) (fkh-2) mRNA, complete cds. 11/11 None
T14G12.4a ENSEMBL cdna:known chromosome:CEL140:X:3740225:3742273:-1 gene:T14G12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180663 2 1 NM_076243 174011_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3740236-3742242(-) 100.0 100.0
Public Domain and Genome References
Ensembl T14G12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_508644 ForKHead transcription factor family member (fkh-2) [Caenorhabditis elegans] gb|AAA82436.1| Forkhead transcription factor family protein 2, isoform a [Caenorhabditis elegans] 1.0E-119
blast CAE68479 Hypothetical protein CBG14280 [Caenorhabditis briggsae] 6.0E-92
blast NP_508645 ForKHead transcription factor family member (fkh-2) [Caenorhabditis elegans] gb|AAL02521.1| Forkhead transcription factor family protein 2, isoform b [Caenorhabditis elegans] 7.0E-88
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 4.00000002044225E-38
Pfam IPR001766 EMBL-EBI Fork head transcription factor 2.1E-64
Sequence
Target Sequence 
>C. ELEGANS:174011_AT
taatccttctttttatggccttcaagttttcttaactcagcatgtgtctcctattagtag
ttcggtgtacctatttttgatttataacttatttcgacctaattaacctacaccaagttt
acaatatttctattcacgggtagcaacttgcaatatttttttcaagaaattgattagcgg
taacttttataactataaatacatgcccctcttccgcttgtcatccttctccctccacaa
caccttataaagatctgtgttccttgctttattacttgttcccattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATCCTTCTTTTTATGGCCTTCAA 692 635 824 Antisense
TTCTTAACTCAGCATGTGTCTCCTA 306 691 852 Antisense
GTGTCTCCTATTAGTAGTTCGGTGT 241 487 867 Antisense
TTCGGTGTACCTATTTTTGATTTAT 40 693 884 Antisense
TTCGACCTAATTAACCTACACCAAG 693 633 916 Antisense
CCTACACCAAGTTTACAATATTTCT 255 269 930 Antisense
CTATTCACGGGTAGCAACTTGCAAT 554 223 953 Antisense
TAACTATAAATACATGCCCCTCTTC 299 635 1013 Antisense
TCCCTCCACAACACCTTATAAAGAT 648 595 1053 Antisense
AGATCTGTGTTCCTTGCTTTATTAC 395 67 1074 Antisense
CTTGCTTTATTACTTGTTCCCATTC 140 219 1086 Antisense
Sequence Source Affymetrix Proprietary Database