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[174004_at]

Description CE20A4 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F22D6.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=M89241 /WB_GENE_ID=F22D6.3 /WP=CE05684 /CHR=1 /FEA=Genomic Cluster /DEF=asparaginyl-tRNA synthetase; CEL20A4 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cm20a4 5prime, mRNA sequence.
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 65      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 57      6grp_total > 65      4grp_total > 56      6grp_highest_sub > Da      6grp_cgc > 46      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3162
1 2 3 4 range
A 3253.2
(-0.4)
P 		3880
(1.2)
P 		4567.6
(3)
P 		2262.6
(-3)
P 		2305
B 3608.9
(-1.3)
P 		4719.6
(1.3)
P 		5424.2
(3)
P 		2899.9
(-3)
P 		2524
C 3288.3
(-0)
P 		4052.1
(2.3)
P 		4305
(3)
P 		2281.3
(-3)
P 		2024
avr 3383.5
(-0.6) 		4217.2
(1.6) 		4765.6
(3) 		2481.3
(-3) 		2284

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1526
1 2 3 4 5 6 range
A 2593.2
(3)
P 		2257.8
(-3)
P 		2496.5
(1.3)
P 		2269.8
(-2.8)
P 		2377.5
(-0.9)
P 		2524.9
(1.8)
P 		335
B 2615.3
(-0.9)
P 		2216.5
(-2.7)
P 		2874.2
(0.3)
P 		2639.9
(-0.8)
P 		2141
(-3)
P 		3471.6
(3)
P 		1331
C 2240.1
(-1)
P 		2160.3
(-1.6)
P 		2676.6
(1.9)
P 		2748.2
(2.4)
P 		1946
(-3)
P 		2838.3
(3)
P 		892
avr 2482.9
(-0.5) 		2211.5
(-2.6) 		2682.4
(1) 		2552.6
(0) 		2154.8
(-3) 		2944.9
(3) 		790
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1169
1 2 3 4 range
A 1184.5
(2.9)
P 		842.2
(0.6)
P 		1194.1
(3)
P 		331.2
(-3)
P 		863
B 1150.8
(2)
P 		1165.6
(2.1)
P 		1256.9
(3)
P 		618
(-3)
P 		639
C 1088.5
(0.8)
P 		1165
(1.2)
P 		1500.4
(3)
P 		384.7
(-3)
P 		1116
avr 1141.3
(1.8) 		1057.6
(1.2) 		1317.1
(3) 		444.6
(-3) 		873

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 817
1 2 3 4 5 6 range
A 478
(-3)
P 		803
(3)
P 		543.7
(-1.8)
P 		575.7
(-1.2)
P 		717.4
(1.4)
P 		614.7
(-0.5)
P 		325
B 910
(-0.9)
P 		737.8
(-2.6)
P 		697.9
(-3)
P 		699.2
(-3)
P 		869.8
(-1.3)
P 		1294.5
(3)
P 		597
C 830.3
(1.2)
P 		659.2
(-1.4)
P 		681.2
(-1.1)
P 		552.7
(-3)
P 		629.7
(-1.8)
P 		950.1
(3)
P 		397
avr 739.4
(-0.7) 		733.3
(-0.8) 		640.9
(-2.4) 		609.2
(-3) 		739
(-0.7) 		953.1
(3) 		344
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174004_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22709
Sequence Type Exemplar sequence
Representative Public ID CE20A4 NCBI
Target Description CE20A4 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F22D6.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=M89241 /WB_GENE_ID=F22D6.3 /WP=CE05684 /CHR=1 /FEA=Genomic Cluster /DEF=asparaginyl-tRNA synthetase; CEL20A4 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cm20a4 5prime, mRNA sequence.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026234(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026234 NCBI Caenorhabditis elegans asparagiNyl tRNA Synthetase family member (nrs-1) (nrs-1) mRNA, complete cds. 11/11 None
F22D6.3a.1 ENSEMBL cdna:known chromosome:CEL140:I:7096170:7098196:-1 gene:F22D6.3 11/11 None
F22D6.3a.2 ENSEMBL cdna:known chromosome:CEL140:I:7096170:7098194:-1 gene:F22D6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7096118-7098121(-) 100.0 100.0
Public Domain and Genome References
Ensembl F22D6.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA95808 Hypothetical protein F22D6.3a [Caenorhabditis elegans] ref|NP_001021405.1| asparagiNyl tRNA Synthetase family member (nrs-1) [Caenorhabditis elegans] sp|Q19722|SYNC_CAEEL Asparaginyl-tRNA synthetase, cytoplasmic (Asparagine--tRNA ligase) (AsnRS) 0.0
blast CAE73099 Hypothetical protein CBG20478 [Caenorhabditis briggsae] 0.0
blast AAA27849 63kd antigen 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004365 EMBL-EBI OB-fold nucleic acid binding domain 3.3E-21
Pfam IPR004364 EMBL-EBI tRNA synthetase, class II (D, K and N) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174004_AT
gatgtgctccgtaaactgatctattttccccatattctattgcctattggtttttttgtt
ccccgtttcagccaagcttacaccgattggccttatctgtcttgttttatatcgttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTGCTCCGTAAACTGATCTATT 119 409 1638 Antisense
GCTCCGTAAACTGATCTATTTTCCC 424 293 1643 Antisense
AAACTGATCTATTTTCCCCATATTC 670 127 1650 Antisense
ATCTATTTTCCCCATATTCTATTGC 603 31 1656 Antisense
TTCCCCATATTCTATTGCCTATTGG 124 699 1663 Antisense
TCTATTGCCTATTGGTTTTTTTGTT 321 615 1673 Antisense
CGTTTCAGCCAAGCTTACACCGATT 385 245 1701 Antisense
CCAAGCTTACACCGATTGGCCTTAT 169 275 1709 Antisense
TTACACCGATTGGCCTTATCTGTCT 676 683 1715 Antisense
TTGGCCTTATCTGTCTTGTTTTATA 581 707 1724 Antisense
TATCTGTCTTGTTTTATATCGTTCT 360 659 1731 Antisense
Sequence Source Affymetrix Proprietary Database